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EC number: 268-732-5 | CAS number: 68134-15-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2003-08-23 to 2003-09-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Deviations:
- no
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of activated sludge: Aeration tank of a municipal biological waste water treatment plant, non-adapted, not pre-conditioned.
- Preparation of inoculum for exposure: Washed twice with tap water.
- Concentration of sludge: 0.2 g/L dry matter in the final mixture - Duration of test (contact time):
- 28 d
- Initial conc.:
- 50 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Aerobic mineral salts medium prepared with double destilled water (conductivity: < 1.5 µS/cm; DOC: < 0.3 mg/L)
- Additional substrate: No, test substance or procedure control as sole organic carbon sources
- pH adjustment: Yes, periodically adjusted to pH 6.5-8.0 with H2SO4, if necessary.
- Test temperature: 22 +/- 0.5 °C
- Continuous darkness: Yes
TEST SYSTEM
- Culturing apparatus: Temperature -controlled dark room
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Aeration with CO2-free air; test vessels were stirred (100 rpm) and aerated with sythetic CO2-free air for a maximum test period of 28 days.
- Measuring equipment: TOC analyzer
- Test performed in closed vessels: Yes
- Details of trap for CO2: Gas-absorption bottles filled with NaOH
SAMPLING
- Sampling frequency: At regular intervals: DOC determinations daily during the first phase of the test, than at least once a week and on the 27th and 28 th day; actually: at the beginning of the test, after 3 hours (0.125 days) and on days 1, 4 , 7, 11, 14, 19, 21, 25, 27, 28
- Sampling method: Not specified
- Sample storage before analysis: No, samples were analysed directly after sampling
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 replicates
- Procedure control: Containing diethylene glycol as reference substance
- Toxicity control: Not performed - Reference substance:
- diethylene glycol
- Preliminary study:
- None
- Key result
- Parameter:
- % degradation (DOC removal)
- Value:
- 85
- Sampling time:
- 28 d
- Details on results:
- More than 80% of the initial concentration of the test item (based on DOC) was biodegraded between day 0 and 7 (without significant lag phase). The highest biodegradation occured after 27 days. In addition to DOC-determination, biodegradation of the test item was followed by CO2 evolution and calculated as % ThCO2. In result, biodegradation determined by CO2 evolution reached 62% after 28 days, which shows that the test item was predominantly transformed into CO2. In conclusion, as more than 70% biodegradation was observed after 28 days, the test item is regarded to be ultimately biodegradable.
- Results with reference substance:
- 99% biodegradation after 14 days of incubation was observed, confirming the suitability of the test system.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable, fulfilling specific criteria
- Conclusions:
- In an inherent biodegradability test according to OECD Guideline 302B, 85% of the initial test item concentration was biodegraded after a contact time of 28 days. Thus, the test item is regarded to be ultimately biodegradable.
- Executive summary:
To assess the inherent biodegradability of the test item, a Zahn-Wellens Test / EMPA Test according to OECD Guideline 302 B was conducted under aerobis static conditions and in compliance with ISO/IEC 17025. Non-adapted and not pre-conditioned activated sludge of a municipal biological waste water treatment plant, non-adapted (final concentration 0.2 g/L test solution) was esposed to an initial test item concentration of 50 mg/L (2 replicates), along with a blank control (2 replicates) and a procedure control with the reference substance diethylene glycol (1 replicate) for an contact time of 28 days. Biodegradation was followed by DOC depletion (analysed with a TOC analyser) and, additionally, by CO2 evolution (CO2 trap: gas-absorption bottles filled with NaOH). The DOC content was determined at the beginning of the test, after 3 hours (0.125 days) and on days 1, 4 , 7, 11, 14, 19, 21, 25, 27, 28. In result, more than 80% of the initial concentration of the test item (based on DOC) was biodegraded between day 0 and 7 (without significant lag phase). The highest biodegradation occured after 27 days. Based on CO2 evolution the biodegradation reached 62% after 28 days, which shows that the test item was predominantly transformed into CO2. In the procedure control, 99% biodegradation after 14 days of incubation was observed, confirming the suitability of the test system. In conclusion, as more than 70% biodegradation of the test item was observed after 28 days, the test item is regarded to be ultimately biodegradable. All validity criteria of the test guidelein were fulfilled.
Reference
Table 1: DOC concentrations of the test suspension, inoculum blank and procedure control and calculation of
degradation data
Inoculum blank (B) | Procedure control with diethylene glycol ® | Test suspension with test material (T)* | |||||
Time (days) | DOC (mg/L) | DOC (mg/L) | DOC net. (mg/L) | Degradation (%) | DOC (mg/L) | DOC net. (mg/L) | Degradation (%) |
0 | 0.1 | 49.8 | 49.7 | - | 49 | 48.9 | - |
0.125 | 1.2 | 50.3 | 49.2 | 2 | 54.9 | 53.7 | 0 |
1 | 0.7 | 49 | 48.3 | 4 | 24.2 | 23.5 | 56 |
4 | 1.3 | 27.7 | 26.4 | 48 | 12.3 | 11 | 80 |
7 | 1.9 | 2.5 | 0.6 | 99 | 11.7 | 9.8 | 82 |
11 | 1.9 | 2.6 | 0.7 | 99 | 11.4 | 9.4 | 82 |
14 | 1.8 | 2.4 | 0.6 | 99 | 10.7 | 8.9 | 83 |
19 | 1.8 | 2.2 | 0.4 | 99 | 10.9 | 9.1 | 83 |
21 | 2 | 2.5 | 0.5 | 99 | 10.7 | 8.7 | 84 |
25 | 2.6 | 2.7 | 0.1 | 100 | 11.2 | 8.6 | 84 |
27 | 2.5 | 2.6 | 0 | 100 | 10.4 | 7.9 | 85 |
28 | 2.4 | 2.5 | 0.1 | 100 | 10.4 | 8 | 85 |
* Mean of two replicates
Table 2: IC concentrations, calculated from the concentrations in the gas absorption bottles, of the test suspension, inoculum blank and procedure control and
corresponding degradation data
Time (days) | Test suspension IC (mg/L) | Procedure control IC (mg/L) | Inoculum blank IC (mg/L) | Biodegradation Test suspension (% ThCO2) | Biodegradation Procedure control (% ThCO2) |
0 | nd | nd | nd | - | - |
7 | 22.0 | 50.0 | 11.7 | 20 | 77 |
14 | 36.8 | 62.1 | 21.5 | 30 | 82 |
21 | 54.6 | 70.2 | 29.3 | 50 | 83 |
28 | 71.0 | 80.5 | 39.4 | 62 | 83 |
Description of key information
Biodegradation in water: screening tests
In a Zahn-Wellens Test / EMPA Test according to OECD Guideline 302 B, the test item was determined to be ultimately biodegradable (85% biodegradation after 28 days).
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable, fulfilling specific criteria
- Type of water:
- freshwater
Additional information
Key study
To assess the inherent biodegradability of the test item, a Zahn-Wellens Test / EMPA Test according to OECD Guideline 302 B was conducted under aerobis static conditions and in compliance with ISO/IEC 17025. Non-adapted and not pre-conditioned activated sludge of a municipal biological waste water treatment plant, non-adapted (final concentration 0.2 g/L test solution) was exposed to an initial test item concentration of 50 mg/L (2 replicates), along with a blank control (2 replicates) and a procedure control with the reference substance diethylene glycol (1 replicate) for an contact time of 28 days. Biodegradation was followed by DOC depletion (analysed with a TOC analyser) and, additionally, by CO2 evolution (CO2 trap: gas-absorption bottles filled with NaOH). The DOC content was determined at the beginning of the test, after 3 hours (0.125 days) and on days 1, 4 , 7, 11, 14, 19, 21, 25, 27, 28. In result, more than 80% of the initial concentration of the test item (based on DOC) was biodegraded between day 0 and 7 (without significant lag phase). The highest biodegradation occured after 27 days. Based on CO2 evolution the biodegradation reached 62% after 28 days, which shows that the test item was predominantly transformed into CO2. In the procedure control, 99% biodegradation after 14 days of incubation was observed, confirming the suitability of the test system. In conclusion, as more than 70% biodegradation of the test item was observed after 28 days, the test item is regarded to be ultimately biodegradable. All validity criteria of the test guideline were fulfilled.
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