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EC number: 946-420-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2013-02-25 to 2013-05-29
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- 23 rd, March 2006 corrected 28th July 2011
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The highest loading (100 mg test item/L) was prepared by dissolving 100.01 mg of the test item in 1000 mL growth medium and stirring for 4 h at room temperature. The other test loadings were prepared by serial dilution of the 100 mg/L solution in growth medium. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Source (laboratory, culture collection): SAG, Culture Collection of Algae at Pflanzenphysiologisches lnstitut of the University at Göttingen, Albrecht von Haller lnstitut, Untere Klarspüle 2, D-37073 Göttingen, Catalog No 61.81 SAG.
- Method of cultivation: The stock cultures are maintained fulfiling the criteria of the OECD guideline (OECD No. 201)
ACCLIMATION
- Culturing media and conditions (same as test or not): identical to test medium - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22.0°C
- pH:
- 7.85-7.86 to 8.22-9.02
- Nominal and measured concentrations:
- 0, 6.25, 12.50, 25.00, 50.00 and 100.00 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: conical flasks
- Type: closed with air-permeable silicone-,sponge caps
- Material, size, headspace, fill volume: Glass flasks with 250 mL fill volume, filled with 100 mL test medium
- Initial cells density: 10,000 cells/mL
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 8
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: All stock solutions and the medium were prepared with purified water processed using an ELGA ‘PURELAB Ultra’.
- Culture medium different from test medium: No
- Light intensity and quality: The culture vessels were continuously illuminated with a light intensity (day light: OSRAM “day light") adjusted to 4440 — 8880 lux close to the surface of the liquid (equivalent to 60- 120 µmol/m²/s²). Light measurements [lux] were made using a cosine (2π) receptor (Ll-189 with radiation sensor, Ll-COR) each day.
EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: electronic particle counter (CASY 1 Model TT, Roche Diagnostics GmbH, Germany) or microscopic counting.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: Non-GLP Study
- Test concentrations: 1, 10, 100 mg/L
- Results used to determine the conditions for the definitive study: Growth rate was maintained until and inhibition occurred at a concentration of 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- routinely checked with 3,5-dichlorophenol
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): not reported - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- EC50: 2.42 (95% confidence limits 1.95 - 3.03 mg/L) - Validity criteria fulfilled:
- yes
- Conclusions:
- The 72 h NOEC of polyglycerin caprylate/caprinate to Pseudokirchneriella subcapitata was 50 mg a.i./L (nominal) based on growth rate and yield.
The 72 hr ErC50 was > 100 mg a.i./L. - Executive summary:
In a 72 hour toxicity study, the cultures of Pseudokirchneriella subcapitata were exposed topolyglycerin caprylate/caprinateat nominal concentrations of 6.25, 12.50, 25.00, 50.00 and 100.00 mg a.i./L under static conditions in accordance with the OECD Test Guideline 201 (2006/2011).
For growth rate, there was no inhibition above 50 % compared to the controls up to the highest test loading. Therefore, the ErL50 was estimated to be > 100 mg a.i./L. The inhibition of yield was above 50 % compared to the controls at the highest treatment and an EyL50 of 99.8 mg a.i./L was calculated. A NOEL of 50 mg/L was estimated for both growth rate and yield
Results Synopsis
Test Organism: Pseudokirchneriella subcapitata
Test Type: Static
72 hr ErC50: > 100 mg a.i./L 95% C.I.: n.d.
72 h ErC10: not determined
72 hr EyC50: 99.8 mg a.i./L 95% C.I.: 59.4 – 561 mg a.i./L
72 h EyC10: 11.2 mg a.i./L 95% C.I.: 0.52 – 22.6 mg a.i./L
72 hr NOEC(yield, growth rate): 50 mg a.i./L Probit Slope: 1.3478
Endpoint(s) Effected: growth rate and yield
Reference
Description of key information
72 h ErC50: > 100 mg a.i./L; 95% C.I.: n.d.
72 h ErC10: not demermined
72 h NOEC: 50 mg a.i./L
(OECD TG 201; RL1; GLP: static, Pseudokirchneriella subcapitata)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 50 mg/L
Additional information
In a 72 hour toxicity study, the cultures of Pseudokirchneriella subcapitata were exposed to polyglycerin caprylate/caprinate at nominal concentrations of 6.25, 12.50, 25.00, 50.00 and 100.00 mg a.i./L under static conditions in accordance with the OECD Test Guideline 201 (2006/2011).
For growth rate, there was no inhibition above 50 % compared to the controls up to the highest test loading. Therefore, the ErL50 was estimated to be > 100 mg a.i./L. The inhibition of yield was above 50 % compared to the controls at the highest treatment and an EyL50 of 99.8 mg a.i./L was calculated. A NOEL of 50 mg/L was estimated for both growth rate and yield.
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