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EC number: 217-370-6 | CAS number: 1825-62-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to analogue justification provided in IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 124 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Conclusions:
- An EC50 (48 h) of 124 mg/L (measured) for toxicity to aquatic invertebrates was determined in a reliable study with a suitable read-across substance conducted according to an appropriate test protocol, and in compliance with GLP.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM Standard E729-88a, Standard Guide for Conducting Acute Toxicity Tests with Fishes, Macroinvertebrates and Amphibians (3).
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Samples were collected from the each test chamber of each concentration
- Sampling method: Test solution samples were collected at mid-depth, placed in glass vials, and analyzed immediately.
- Sample storage conditions before analysis: No - Vehicle:
- no
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Waterflea
- Source: inhouse culture, Wildlife International, Ltd. Cultures Easton, Maryland 21601
- Age at study initiation (mean and range, SD): Neonates, <24 hours old
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: mixture of yeast, Cerophyll® and trout chow, as well as a suspension of the freshwater green alga, Selenastrum capricornutum
- Feeding frequency: daily
- Health during acclimation (any mortality observed): The adults showed no signs of disease or stress during the holding period - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 129 mg/L as CaCO3
- Test temperature:
- 20 ± 1ºC
- pH:
- 8.3
- Nominal and measured concentrations:
- Nominal: 63, 125, 250, 500 and 1000 mg/L.
The mean measured concentrations for the study were 60, 116, 207, 447 and 905 mg/L, representing 95, 93, 83, 89 and 91% of nominal concentrations, respectively. The results of the study were based on the mean measured test concentrations. - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 250-mL glass beakers containing approximately 200 mL of test solution
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Freshwater obtained from a well approximately 40 meters deep located on the Wildlife International, Ltd. site. The well water is characterized as moderately-hard water.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod:16 hours of light and 8 hours of darkness
- Light intensity:Fluorescent light bulbs that emit wavelengths similar to natural sunlight (Colortone 50) were used, Light intensity at test initiation was 131 lux
EFFECT PARAMETERS MEASURED : All organisms were observed periodically to determine the numbers of mortalities and immobile
organisms in each control and treatment group. The numbers of individuals exhibiting clinical signs of toxicity or abnormal behavior also were evaluated. Observations were made approximately 4, 24 and 48 hours after test initiation. - Reference substance (positive control):
- not specified
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 124 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Reported statistics and error estimates:
- The mortality/immobility data were analyzed using the computer program of C. E. Stephan.
The program was designed to calculate the EC50 value and the 95% confidence interval by probit analysis,
the moving average method, and binomial probability with nonlinear interpolation. In this study,
the probit method was used to calculate the 48-hour EC50 value. There was <50% mortality or immobility
in any treatment group at 24 hours, which precluded the statistical calculation of a 24-hour EC50 value.
Therefore, the 24-hour EC50 value and the NOEC were determined by visual inspection of the mortality,
immobility and observation data. - Conclusions:
- An EC50 (48 h) of 124 mg/L (measured) for toxicity to aquatic invertebrates was determined in a reliable study substance conducted according to an appropriate test protocol, and in compliance with GLP.
Referenceopen allclose all
Cumulative Mortality, Immobility and Observations:
Mean Measured Concentration (mg/L)
|
Rep. |
Daphnia/ Replicate |
Cumulative Number Dead 0 0 |
Number Immobile |
Effects1 |
Percent Dead and Immobile |
Negative Control |
A B |
10 10 |
0 0 |
0 1 |
10 AN 9 AN |
5 |
60 |
A B |
10 10 |
0 0 |
3 3 |
7 AN 7 AN |
30 |
116 |
A B |
10 10 |
2 5 |
4 2 |
4AN (2Q) 3AN |
65 |
207 |
A B |
10 10 |
4 4 |
1 0 |
5AN (2Q) 6AN (3Q) |
45 |
447 |
A B |
10 10 |
4 6 |
4 2 |
2AN (10Q) 1AN,1C (6Q) |
80 |
905 |
A B |
10 10 |
3 5 |
5 4 |
2C (7Q) 1AN (4Q) |
85 |
1Observed effects: AN = appear normal; C = lethargic; Q = daphnia trapped on surface of test solution, then submerged below water surface and observed.
EC50 values:
Time |
EC50 (mg/L) |
95% Confidence Interval (mg/L) |
Statistical Method |
24 Hours |
>905 |
--1 |
NA2 |
48 Hours |
124 |
51 - 203 |
Probit |
1Confidence limits could not be calculated with the mortality/immobility data obtained.2NA = Not applicable. Estimation made by visual inspection of the mortality/immobility data. |
Daily observations of mortality, immobility and other clinical signs of toxicity observed during the
test are presented in the Table above. All daphnids in the negative control group appeared healthy and normal
throughout the test, with the exception of one immobile daphnia at test termination. In the 60, 116, 207,
447 and 905 mg/L treatment groups, mortality/immobility at 48 hours was 30, 65, 45, 80 and 85%,
respectively. At 48-hours, daphnia trapped on the water surface were observed in every treatment group
except the negative control and 60 mg/L, with more daphnia being trapped at the higher concentrations,
although a surface slick was not observed at test initiation or termination, initial observations, or during the
transfer of daphnia to new test solutions at 24-hours in any of the test concentrations.
CONCLUSIONS
Daphnia magna were exposed under static-renewal test conditions for 48 hours to five
concentrations of trimethylsilanol ranging in concentration from 60 to 905 mg/L. Based on the mean
measured test concentrations, the 48-hour EC50 value was 124 mg/L, with a 95% confidence interval of
51 to 203 mg/L. The slope of the concentration-response curve was 1.2. The NOEC was < 60 mg/L, the
lowest treatment group tested.
Cumulative Mortality, Immobility and Observations:
Mean Measured Concentration (mg/L)
|
Rep. |
Daphnia/ Replicate |
Cumulative Number Dead 0 0 |
Number Immobile |
Effects1 |
Percent Dead and Immobile |
Negative Control |
A B |
10 10 |
0 0 |
0 1 |
10 AN 9 AN |
5 |
60 |
A B |
10 10 |
0 0 |
3 3 |
7 AN 7 AN |
30 |
116 |
A B |
10 10 |
2 5 |
4 2 |
4AN (2Q) 3AN |
65 |
207 |
A B |
10 10 |
4 4 |
1 0 |
5AN (2Q) 6AN (3Q) |
45 |
447 |
A B |
10 10 |
4 6 |
4 2 |
2AN (10Q) 1AN,1C (6Q) |
80 |
905 |
A B |
10 10 |
3 5 |
5 4 |
2C (7Q) 1AN (4Q) |
85 |
1Observed effects: AN = appear normal; C = lethargic; Q = daphnia trapped on surface of test solution, then submerged below water surface and observed.
EC50 values:
Time |
EC50 (mg/L) |
95% Confidence Interval (mg/L) |
Statistical Method |
24 Hours |
>905 |
--1 |
NA2 |
48 Hours |
124 |
51 - 203 |
Probit |
1Confidence limits could not be calculated with the mortality/immobility data obtained.2NA = Not applicable. Estimation made by visual inspection of the mortality/immobility data. |
Daily observations of mortality, immobility and other clinical signs of toxicity observed during the
test are presented in the Table above. All daphnids in the negative control group appeared healthy and normal
throughout the test, with the exception of one immobile daphnia at test termination. In the 60, 116, 207,
447 and 905 mg/L treatment groups, mortality/immobility at 48 hours was 30, 65, 45, 80 and 85%,
respectively. At 48-hours, daphnia trapped on the water surface were observed in every treatment group
except the negative control and 60 mg/L, with more daphnia being trapped at the higher concentrations,
although a surface slick was not observed at test initiation or termination, initial observations, or during the
transfer of daphnia to new test solutions at 24-hours in any of the test concentrations.
CONCLUSIONS
Daphnia magna were exposed under static-renewal test conditions for 48 hours to five
concentrations of trimethylsilanol ranging in concentration from 60 to 905 mg/L. Based on the mean
measured test concentrations, the 48-hour EC50 value was 124 mg/L, with a 95% confidence interval of
51 to 203 mg/L. The slope of the concentration-response curve was 1.2. The NOEC was < 60 mg/L, the
lowest treatment group tested.
Description of key information
EC50 (48 h) 124 mg/L (measured, OECD 202)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 124 mg/L
Additional information
There are no data available on short-term toxicity to aquatic invertebrates for ethoxytrimethylsilane (CAS 1825-62-3). In accordance with Regulation (EC) No 1907/2006 Annex XI, 1.5 “Grouping of substances and read-across” and in accordance with the read across assessment framework (RAAF, ECHA 2017) a read-across from the hydrolysis product trimethylsilanol (CAS 1066-40-6) has been applied to support the environmental hazard assessment of ethoxytrimethylsilane. Details on the read across justification can be found in the attached justification in the respective target entry and in the overall justification for grouping of substances attached in IUCLID Section 13.
The study with the analogue substance trimethylsilanol (CAS 1066-40-6) was performed according to OECD 202 (GLP). Daphnia magna was exposed to the substance up to a concentration of 1000 mg/L under semi-static conditions. The test concentrations remained stable for the duration of the study. The mean measured concentrations were in the range of 95-103% of nominal. Immobilisation was recorded after 48 h resulting in an EC50 (48 h) 124 mg/L.
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