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EC number: 268-329-4 | CAS number: 68052-51-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14 March 2017 - 13 April 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- 17 July 1992
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- - Soluble in water: yes
- Stability in water: stable - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of activated sludge: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Storage conditions: under continuous aeration until further treatment
- Pretreatment: before use, the sludge was allowed to settle (31 minutes) and the supernatant liquid was used as inoculum at the amount of 10 mL/L of mineral medium.
- Concentration of sludge: 4.4 g suspended solids/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 21 mg/L
- Based on:
- test mat.
- Initial conc.:
- 12 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to OECD 301B
- Test temperature: 22-24°C
- pH: 7.6 (start), 7.7 (day 14), 7.6 (day 28)
- pH adjusted: no
- Aeration of dilution water: yes
- Suspended solids concentration: not indicated: 10 mL supernatant of a settled solution containing 4.4 g suspended solids/L suspension was taken to use as inoculum.
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 2 litre brown coloured glass bottles
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: by synthetic air (CO2 < 1 ppm; at a rate of approx. 1-2 bubbles per second/ ca. 30-100 mL/min)
- Details of trap for CO2: three bottles filled with 100 mL 0.0125 M Ba(OH)2) were connected in series to the exit air line of each test bottle.
- Test performed in open system: no
- Other: phenolphthalein (1% solution in ethanol, Merck) was used as pH-indicator.
SAMPLING
- Sampling frequency: titrations were made on day 2, 5, 7, 9, 15, 19, 23, 27, and 29. Titrations for the positive and toxicity control were made over a period of at least 14 days. Each time the CO2-absorber nearest to the test bottle was removed for titration; each of the remaining two absorbers was moved one position in the direction of the test bottle. A new CO2-absorber was placed at the far end of the series.
- Sampling method: the amount of CO2 produced was determined by titrating Ba(OH)2 with 0.05 M standardized HCl
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 2 bottles
- Abiotic sterile control: no
- Toxicity control: yes, 1 bottle
- Other: positive control containing reference item and inoculum (1 bottle)
STATISTICAL METHODS: no statistical analysis was used - Reference substance:
- acetic acid, sodium salt
- Remarks:
- Purity: 99.6%
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Remarks:
- mean of duplicate bottles
- Value:
- 4
- Sampling time:
- 28 d
- Details on results:
- - The ThCO2 of the test substance was calculated to be 2.07 mg CO2/mg. The ThCO2 of the reference item was calculated to be 1.07 CO2/mg.
- The test substance biodegraded for 0% and for 8% in duplicate bottles respectively.
- In the toxicity control, more than 25% biodegradation occurred within 14 days (40%, based on ThCO2). Therefore, the test item was assumed not to inhibit microbial activity. - Results with reference substance:
- Sodium acetate was biodegraded for 86% after 14 days.
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on results' section.
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- TBEAES was not readily biodegradable under the conditions of the modified Sturm test, according to OECD 301B and GLP principles.
- Executive summary:
In a Modified Sturm Test, according to OECD guideline 301 B and GLP principles, TBEAES DRY was assessed for its ''ready biodegradability'' potential. The test substance was tested in duplicate at a target concentration of 21 mg/L, corresponding to 12 mg TOC/L. The exposure period was 28 days. Two inoculum blanks, a positive control and a toxicity control were included. Since the test substance was well soluble in water, the test media were prepared using a stock solution of 1 g/L in Milli-RO water by dissolving 501.03 mg of the test substance in 500 mL water and stirring for 90 minutes.
CO2 measurements showed that the test substances biodegraded for 0% and 8%, in the duplicate bottles measured. Because the criterion for ready biodegredability was not met, TBEAES DRY was concluded to be not readily biodegradable under the conditions of this test.
The test substance was found not to inhibit microbial activity and all validity criteria were met, thus the study was considered to be valid.
Reference
Table 1 CO2 production in the blank
Day |
HCl (0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
|
|
Ba(OH)21) |
Blank (mean) |
|
||||
2 |
48.76 |
45.71 |
3.05 |
3.4 |
3.4 |
|
5 |
48.82 |
45.09 |
3.72 |
4.1 |
7.5 |
|
7 |
48.98 |
45.49 |
3.49 |
3.8 |
11.3 |
|
9 |
51.21 |
47.76 |
3.45 |
3.8 |
15.1 |
|
15 |
50.15 |
47.18 |
2.97 |
3.3 |
18.3 |
|
19 |
50.14 |
46.31 |
3.83 |
4.2 |
22.6 |
|
23 |
50.46 |
45.73 |
4.72 |
5.2 |
27.8 |
|
27 |
51.68 |
46.50 |
5.18 |
5.7 |
33.5 |
|
292) |
51.27 |
46.78 |
4.49 |
4.9 |
38.4 |
|
292) |
51.08 |
48.26 |
2.82 |
3.1 |
41.5 |
|
292) |
50.72 |
50.05 |
0.67 |
0.7 |
42.2 |
|
1): "Strength" of untreated 0.0125 M Ba(OH)2solution 2): CO2measured on day 29 is actually part of CO2production of day 28, since microbial activity was ended on day 28 by addition of HCl. |
Table 2 CO2 Production and Percentage Biodegradation of the Positive Control Item
Day |
HCl (0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation1) (%) |
||
Blank (mean) |
Positive control |
||||||
2 |
45.71 |
37.81 |
7.90 |
8.7 |
8.7 |
10 |
|
5 |
45.09 |
18.96 |
26.13 |
28.7 |
37.4 |
44 |
|
7 |
45.49 |
33.62 |
11.87 |
13.1 |
50.5 |
59 |
|
9 |
47.76 |
40.91 |
6.85 |
7.5 |
58.0 |
68 |
|
152) |
47.18 |
33.37 |
13.81 |
15.2 |
73.2 |
86 |
|
1): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of sodium acetate: 85.6 mg CO2/2L. 2): CO2measured on day 15 is actually part of CO2production of day 14, since microbial activity was ended on day 14 by addition of HCl. |
|
Table 3 CO2 Production and Percentage Biodegradation of the Test Item (Bottle A)
Day |
HCl (0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation1) (%) |
||
Blank (mean) |
Bottle A |
||||||
2 |
45.71 |
47.00 |
0.00 |
0.0 |
0.0 |
0 |
|
5 |
45.09 |
45.78 |
0.00 |
0.0 |
0.0 |
0 |
|
7 |
45.49 |
46.33 |
0.00 |
0.0 |
0.0 |
0 |
|
9 |
47.76 |
47.65 |
0.10 |
0.1 |
0.1 |
0 |
|
15 |
47.18 |
46.48 |
0.70 |
0.8 |
0.9 |
1 |
|
19 |
46.31 |
45.36 |
0.95 |
1.0 |
1.9 |
2 |
|
23 |
45.73 |
45.06 |
0.67 |
0.7 |
2.7 |
3 |
|
27 |
46.50 |
45.71 |
0.79 |
0.9 |
3.5 |
4 |
|
292) |
46.78 |
44.75 |
2.03 |
2.2 |
5.8 |
7 |
|
292) |
48.26 |
47.64 |
0.62 |
0.7 |
6.5 |
7 |
|
292) |
50.05 |
49.66 |
0.39 |
0.4 |
6.9 |
8 |
|
1): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of the test item: 87.1 mg CO2/2L. 2): CO2measured on day 29 is actually part of CO2production of day 28, since microbial activity was ended on day 28 by addition of HCl. |
|
Table 4 CO2 Production and Percentage Biodegradation of the Test Item (Bottle B)
Day |
HCl(0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation1) (%) |
||
Blank (mean) |
Bottle A |
||||||
2 |
45.71 |
47.60 |
0.00 |
0.0 |
0.0 |
0 |
|
5 |
45.09 |
46.13 |
0.00 |
0.0 |
0.0 |
0 |
|
7 |
45.49 |
47.38 |
0.00 |
0.0 |
0.0 |
0 |
|
9 |
47.76 |
48.14 |
0.00 |
0.0 |
0.0 |
0 |
|
15 |
47.18 |
47.31 |
0.00 |
0.0 |
0.0 |
0 |
|
19 |
46.31 |
46.80 |
0.00 |
0.0 |
0.0 |
0 |
|
23 |
45.73 |
47.03 |
0.00 |
0.0 |
0.0 |
0 |
|
27 |
46.50 |
47.21 |
0.00 |
0.0 |
0.0 |
0 |
|
292) |
46.78 |
46.80 |
0.00 |
0.0 |
0.0 |
0 |
|
292) |
48.26 |
48.96 |
0.00 |
0.0 |
0.0 |
0 |
|
292) |
50.05 |
49.81 |
0.24 |
0.3 |
0.3 |
0 |
|
1): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of the test item: 87.1 mg CO2/2L. 2): CO2measured on day 29 is actually part of CO2production of day 28, since microbial activity was ended on day 28 by addition of HCl. |
|
Table 5 CO2 Production and Percentage Biodegradation of the Toxicity Control
Day |
HCl (0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation1) (%) |
|
Blank (mean) |
Toxicity control |
|||||
2 |
45.71 |
41.63 |
4.08 |
4.5 |
4.5 |
3 |
5 |
45.09 |
22.43 |
22.66 |
24.9 |
29.4 |
17 |
7 |
45.49 |
35.04 |
10.45 |
11.5 |
40.9 |
24 |
9 |
47.76 |
39.44 |
8.32 |
9.1 |
50.0 |
29 |
152) |
47.18 |
30.41 |
16.77 |
18.4 |
68.5 |
40 |
1): Calculated as the ratio between CO2produced (cumulative) and the sum of the ThCO2of the test item and positive control: 172.7 mg CO2/2L (ThCO2test item: 87.1 mg CO2/2L + ThCO2sodium acetate: 85.6 mg CO2/2L). 2): CO2measured on day 15 is actually part of CO2production of day 14, since microbial activity was ended on day 14 by addition of HCl. |
Description of key information
Not readily biodegradable under the conditions of a Modified Sturm Test, according to OECD guideline 301 B and GLP principles.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
In a Modified Sturm Test, according to OECD guideline 301 B and GLP principles, TBEAES DRY was assessed for its ''ready biodegradability'' potential. The test substance was tested in duplicate at a target concentration of 21 mg/L, corresponding to 12 mg TOC/L. The exposure period was 28 days. Two inoculum blanks, a positive control and a toxicity control were included. Since the test substance was well soluble in water, the test media were prepared using a stock solution of 1 g/L in Milli-RO water by dissolving 501.03 mg of the test substance in 500 mL water and stirring for 90 minutes.
CO2 measurements showed that the test substances biodegraded for 0% and 8%, in the duplicate bottles measured. Because the criterion for ready biodegredability was not met, TBEAES DRY was concluded to be not readily biodegradable under the conditions of this test.
The test substance was found not to inhibit microbial activity and all validity criteria were met, thus the study was considered to be valid.
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