Sage, Salvia officinalis, ext.

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

calculation (if not (Q)SAR)

Remarks:

Estimated by calculation

Adequacy of study:

key study

Study period:

From 2017/11/03 to 2017/11/03

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Calculation method is used; calculation method applicable for that endpoint.

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Deviations:

yes

Remarks:

calculation method

Principles of method if other than guideline:

The acute toxicity to aquatic algae was determined using a validated QSAR for the Mode of Action in question. The first step of the iSafeRat mixture toxicity calculation employs phase equilibrium thermodynamics in order to determine the concentrations of each constituent within the WAF. This fraction equates to the analysable fraction of a WAF study.
Within the WAF, the constituents also partition between themselves further reducing the bioavailable fraction and thus the toxicity of the mixture compared to the individual constituents. In the calculation the second step is to remove this non-bioavailable fraction.
The final step is to determine the truly bioavailable fraction of the WAF per constituent. The ErC50s of each constituent are already known from literature or calculated using the iSafeRat QSAR model. An additivity approach (based on Chemical Activity of each constituent) is used in order to calculate the Effective Loading rate of the WAF.
The method has been validated using data derived from 72-hour ErC50 tests on aquatic algae, for which the concentrations of the test item had been determined by chemical analyses over the test period. Further to this the effective loading rate of the WAF is determined by using a series of calculation steps using phase equilibrium thermodynamics and excluding the non-bioavailable fraction.

GLP compliance:

not specified

Specific details on test material used for the study:

Not applicable.

Analytical monitoring:

not required

Details on sampling:

Not applicable.

Vehicle:

no

Details on test solutions:

Not appliable.

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

Not appliable.

Test type:

other: calculation method

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Remarks on exposure duration:

72h-ErL50 (effective loading rate of WAF)

Post exposure observation period:

Not appliable.

Hardness:

Hardness is not a necessary component of the WAF calculation.

Test temperature:

The Temperature is not a necessary component of the WAF calculation.

pH:

The pH is not a necessary component of the WAF calculation .

Dissolved oxygen:

The oxygen concentration is not a necessary component of the WAF calculation.

Salinity:

Salinity is not a necessary component of the WAF calculation.

Nominal and measured concentrations:

The calculation determines measured concentrations.

Details on test conditions:

Calculation method.

Reference substance (positive control):

not required

Duration:

72 h

Dose descriptor:

other: ErL50

Remarks:

effective loading rate

Effect conc.:

18 mg/L

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Based on the typical composition provided by the supplier of the substance

Details on results:

Not applicable.

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

Not applicable

Analytically Measured Aqueous Phase (AMAP) calculated at the predicted EL50:

constituents	concentration in the WAF (mg.L-1)
constituent 1	4.4
constituent 2	3.2
constituent 3	1.7
constituent 4	1.2
constituent 5	0.63
constituent 6	0.0027
constituent 7	0.25
constituent 8	0.0041
constituent 9	0.68
constituent 10	0.28
constituent 11	0.2
constituent 12	0.14
constituent 13	0.054
constituent 14	0.25
constituent 15	0.071
constituent 16	0.088
constituent 17	0.088
constituent 18	0.07
constituent 19	0.021

Validity criteria fulfilled:

yes

Conclusions:

72h-ErL50 for typical composition of Sage Dalmatian Oil is of 18 mg test item/L.

Executive summary:

Sage Dalmatian Oil is a Natural Complex Substance (UVCB) with a well-defined composition. Its acute toxicity to aquatic algae has been investigated using an in-house calculation method that replaces an OECD 201 study and guideline for Testing of Chemicals No. 23 (i.e. WAF conditions). The “typical” composition proposed by the supplier of the substance has been investigated.

The first step of the iSafeRat mixture toxicity calculation employs phase equilibrium thermodynamics in order to determine the concentrations of each constituent within the WAF. This fraction equates to the analyzable fraction of a WAF study.

Within the WAF, the constituents also partition between themselves further reducing the bioavailable fraction and thus the toxicity of the mixture compared to the individual constituents. In the calculation the second step is to remove this non-bioavailable fraction.

The final step is to determine the truly bioavailable fraction of the WAF per constituent. The ErC50s of each constituent are already known from literature or predicted using the iSafeRat QSAR model. Each value has been included as a supporting study in the IUCLID. An additivity approach (based on Chemical Activity of each constituent) is used in order to calculate the Effective Loading rate of the WAF.

Using this approach, the 72-h ErL50 was 18 mg test material/L for the typical composition of Sage Dalmatian Oil. ErL50 was based on growth rate.

Based on the results of this study, Sage dalamatian oil would not be classified as acute 1 to aquatic organisms in accordance with the classification of the CLP.

This toxicity study is acceptable and can be used for that endpoint.

 

Results Synopsis

Test Type: Calculation method

ErL50: 18 mg test material/L for typical composition

Description of key information

72h-ErL50 for typical composition of Sage Dalmatian oil is of 18 mg test item /L.

Based on the results of this study, Sage Dalmatian oil would not be classified as acute 1 to aquatic organisms in accordance with the classification of the CLP.

Key value for chemical safety assessment

EC50 for freshwater algae:

18 mg/L

Additional information

The acute toxicity to aquatic algae of the UVCB Sage Dalmatian Oil has been investigated using a reliable in-house calculation method that replaces an OECD 201 study and guideline for Testing of Chemicals No. 23 (i.e. WAF conditions). The “typical” composition proposed by the supplier of the substance has been investigated.

The first step of the iSafeRat mixture toxicity calculation employs phase equilibrium thermodynamics in order to determine the concentrations of each constituent within the WAF. This fraction equates to the analyzable fraction of a WAF study. Within the WAF, the constituents also partition between themselves further reducing the bioavailable fraction and thus the toxicity of the mixture compared to the individual constituents. In the calculation the second step is to remove this non-bioavailable fraction. The final step is to determine the truly bioavailable fraction of the WAF per constituent. The ErC50s of each constituent are already known from literature or predicted using the iSafeRat QSAR model. Each value has been included as a supporting study in the IUCLID. An additivity approach (based on Chemical Activity of each constituent) is used in order to calculate the Effective Loading rate of the WAF.