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Diss Factsheets
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EC number: 306-227-4 | CAS number: 96690-34-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data based on the inhibition control of a ready biodegradability study. This approach is in accordance with the Guidance on information requirements and chemical safety assessment (Chapter R.7b: Endpoint specific guidance, ECHA 2017).
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- adopted July 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- No 440/2008, 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Ministerium für Arbeit, Integration und Soziales des Landes Nordrhein-Westfalen, Germany
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- 25 mg of the test item and 25 mg of the reference compound were weighed out on aluminium foil and both were added to the test flasks, filled with 200 mL of mineral medium. Afterwards the flask volume was made up to 250 mL with mineral medium containing the inoculum to give a test concentration of 100 mg test item and reference compound/L. - Test organisms (species):
- other: activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum: mixed population of aquatic microorganisms (activated sludge), aeration tank of a wastewater plant treating predominantly domestic sewage (Wupper area water authority, WWTP Odenthal), date of collection: 27 Aug 2018
- Storage conditions: Before use, the inoculum was stored for one day at room temperature under continuous stirring with aeration
- Preparation of inoculum for exposure: The sludge was washed twice by adding mineral medium and centrifuging for 10 min at 2000 rpm and 20 °C and decanting off the supernatant.
- Pretreatment: An aliquot of the wet sludge was dried in order to determine the wet weight / dry weight ratio of the sludge and to prepare a stock suspension (activated sludge) of 3 g dw/L. The calculated amount of sludge, needed to achieve 300 mL of this stock suspension, was dissolved in mineral medium and then filled up to a defined end volume.
- Concentration of sludge: 30 mg/L suspended solids - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Test temperature:
- 22 ± 1 °C
- Details on test conditions:
- - Composition of medium: The mineral medium was prepared from stock solutions of mineral components according to the guideline.
- Test temperature: 22 ± 1 °C
- pH: 7.4 ± 0.2
- pH adjusted: no
- Suspended solids concentration: 30 mg/L suspended solids
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: OxiTopControl System (WTW)
- Number of culture flasks/concentration: 3 for test item concentration 100 mg/L
- Method used to create aerobic conditions: Test vessels were stirred by an inductive stirring system for a maximum test period of 56 days.
- Measuring equipment: During the test the O2 uptake was continuously measured with a manometrie BOD measuring device (OxiTop®-C measuring head (WTW Wissenschaftlich-Technische Werkstätten GmbH & Co. KG, Weilheim, Germany).
SAMPLING
- Sampling frequency: continously
CONTROL AND BLANK SYSTEM
- Inoculum blank: 3 replicates
- Toxicity control: 1 replicates
- Reference control: 2 replicates
STATISTICAL METHODS: not applicable - Reference substance (positive control):
- yes
- Remarks:
- sodium benzoate
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Result of toxicity control from ready biodegradability test
- Results with reference substance (positive control):
- The reference compound sodium benzoate showed 88% degradation after 14 days.
- Validity criteria fulfilled:
- yes
Reference
The toxicity control attained 34% degradation after 14 days of incubation.
“If in a toxicity test, containing both the test and reference substance, less than 35% degradation (based on total DOC) or less than 25% (based on total ThOD or ThCO2) occurred within 14 days, the test substance can be assumed to be inhibitory.” (OECD Guideline 301)
Since more than 25% degradation occurred in the toxicity control, the substance is with high probability not toxic to aquatic microorganisms. The test item concentration in the toxicity control of 100 mg/L can be used as NOEC value for the toxicity to aquatic microorganisms.
Description of key information
NOEC (14 d) ≥ 100 mg/L (toxicity control, OECD 301F)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
One study investigating the toxicity to activated sludge microorganisms is available. The endpoint is based on the inhibition control of a ready biodegradability study. In this study according to OECD 301F a 14 d-NOEC of ≥ 100 mg/L could be determined, based on the toxicity control.
One study investigating the biodegradation of the test item was investigated in an O2-consumption test according to OECD guideline 301F using domestic activated sludge as inoculum (CURRENTA GmbH & Co., 2019). This test also consisted of a toxicity control. The toxicity control was tested with a test concentration of the test item of 100 mg/L (+ reference item at a concentration of 100 mg/L). The toxicity control attained 34% degradation after 14 days of incubation. If in a toxicity control, containing both the test substance and a reference compound, more than 25% degradation (based on total ThOD) occurred within 14 days, the test substance can be assumed to be non-inhibitory to microbial organisms. This approach is in line with the OECD Guideline 301 as well as the ECHA Guidance R.7b (ECHA, 2017). Thus, the test item concentration in the toxicity control of 100 mg/L can be used as NOEC value for the toxicity to aquatic microorganisms (NOEC (14 d) ≥ 100 mg/L).
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