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EC number: 237-360-5 | CAS number: 13762-51-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
Under physiological conditions, potassium tetrahydroborate decomposes to boric acid and borate salt, respectively. Boric acid and borax and thus potassium tetrahydroborate as well can be stated as non-mutagenic under the test conditions.
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Materials and methods are described in detail. The test procedure follows the general procedure for the AMES-Test. Peer-reviewed publication that meets basic scientific principles.
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Materials and methods are described in detail. The test procedure follows the general procedure for the AMES-Test. Peer-reviewed publication that meets basic scientific principles.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
- Specific details on test material used for the study:
- Boric acid and Borax were used as test materials.
- Target gene:
- Histidine
- Species / strain / cell type:
- S. typhimurium TA 98
- Species / strain / cell type:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- 0.01, 0.1, 1, 10, 100 µg/plate for boric acid and borax
In preliminary assays, borax and boric acid showed mutagenic activity at a level of l μglplate; therefore, a range of doses from 0.01 to 100 μg/plate was chosen for study. - Vehicle / solvent:
- water
- Untreated negative controls:
- yes
- Remarks:
- spontaneous revertants
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- benzo(a)pyrene
- Details on test system and experimental conditions:
- The bacterial strains (T A98 and TA 100) were acquired from Dr. B. N. Ames (Biochemistry Department, University of California, Berkeley, CA).
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Conclusions:
- Boric acid and borax can be stated as non-mutagenic under the test conditions.
- Executive summary:
The boron compounds failed to produce a mutagenic response in either of the test strains. The revertant colonies produced per plate (23 to 44 and 78 to 127 for TA98 and TA 100, respectively) were not significantly different from the rate of spontaneous revertants for both strains tested.
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Under physiological conditions, potassium tetrahydroborate decomposes to boric acid and borate salt, respectively. Thus the genetic toxicity of boric acid should be determined to evaluate the mutegenic potential of potassium tetrahydroborate.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Materials and methods are described in detail. The test procedure follows the general procedure for the AMES-Test.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
- Specific details on test material used for the study:
- Boric acid and Borax were used as test materials.
- Target gene:
- Histidine
- Species / strain / cell type:
- S. typhimurium TA 98
- Species / strain / cell type:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Test concentrations with justification for top dose:
- 0.01, 0.1, 1, 10, 100 µg/plate for boric acid and borax
In preliminary assays, borax and boric acid showed mutagenic activity at a level of l μglplate; therefore, a range of doses from 0.01 to 100 μg/plate was chosen for study. - Vehicle / solvent:
- water
- Untreated negative controls:
- yes
- Remarks:
- spontaneous revertants
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- benzo(a)pyrene
- Details on test system and experimental conditions:
- The bacterial strains (T A98 and TA 100) were acquired from Dr. B. N. Ames (Biochemistry Department, University of California, Berkeley, CA).
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Conclusions:
- Under physiological conditions, potassium tetrahydroborate decomposes to boric acid and borate salt, respectively. Boric acid and borax and thus potassium tetrahydroborate as well can be stated as non-mutagenic under the test conditions.
- Executive summary:
The boron compounds failed to produce a mutagenic response in either of the test strains. The revertant colonies produced per plate (23 to 44 and 78 to 127 for TA98 and TA 100, respectively) were not significantly different from the rate of spontaneous revertants for both strains tested.
Referenceopen allclose all
Table 1. Response of Salmonella test strains to boron and positive control compounds with and without S-9 activation*
Material | Conc. (µg/plate) | TA98 with S-9 | TA98 without S-9 | TA100 with S-9 | TA100 without S-9 |
Borax | 0.01 | 34±4 | 30±2 | 92±11 | 107±35 |
Borax | 0.1 | 29±1 | 30±2 | 91±6 | 98±16 |
Borax | 1 | 34±7 | 42±4 | 116±7 | 81±5 |
Borax | 10 | 37±8 | 32±5 | 121±24 | 78±12 |
Borax | 100 | 43±6 | 31±5 | 113±8 | 87±9 |
Boric acid | 0.01 | 37±3 | 29±7 | 104±23 | 122±14 |
Boric acid | 0.1 | 35±6 | 29±3 | 103±12 | 112±15 |
Boric acid | 1 | 44±4 | 32±11 | 108±18 | 126±10 |
Boric acid | 10 | 43±4 | 42±1 | 126±3 | 127±9 |
Boric acid | 100 | 40±9 | 23±7 | 108±11 | 127±3 |
Benzo[a]pyrene | 2 | 142±22 | 36±8 | 327±19 | 125±9 |
Benzo[a]pyrene | 4 | 322±17 | 31±4 | 691±7 | 111±15 |
Benzo[a]pyrene | 6 | 540±57 | 26±3 | 780±59 | 110±21 |
Sodium azide | 1 | 54±6 | 39±4 | 101±69 | 481±7 |
Sodium azide | 5 | 43±6 | 35±6 | 395±21 | 896±23 |
Sodium azide | 10 | 36±12 | 46±13 | 786±130 | 986±50 |
Spontaneous revertants | - | 39±1 | 37±8 | 111±23 | 97±11 |
* Data are expressed as number of revertant colonies per plate per strain (means ± SD).
Table 1. Response of Salmonella test strains to boron and positive control compounds with and without S-9 activation*
Material | Conc. (µg/plate) | TA98 with S-9 | TA98 without S-9 | TA100 with S-9 | TA100 without S-9 |
Borax | 0.01 | 34±4 | 30±2 | 92±11 | 107±35 |
Borax | 0.1 | 29±1 | 30±2 | 91±6 | 98±16 |
Borax | 1 | 34±7 | 42±4 | 116±7 | 81±5 |
Borax | 10 | 37±8 | 32±5 | 121±24 | 78±12 |
Borax | 100 | 43±6 | 31±5 | 113±8 | 87±9 |
Boric acid | 0.01 | 37±3 | 29±7 | 104±23 | 122±14 |
Boric acid | 0.1 | 35±6 | 29±3 | 103±12 | 112±15 |
Boric acid | 1 | 44±4 | 32±11 | 108±18 | 126±10 |
Boric acid | 10 | 43±4 | 42±1 | 126±3 | 127±9 |
Boric acid | 100 | 40±9 | 23±7 | 108±11 | 127±3 |
Benzo[a]pyrene | 2 | 142±22 | 36±8 | 327±19 | 125±9 |
Benzo[a]pyrene | 4 | 322±17 | 31±4 | 691±7 | 111±15 |
Benzo[a]pyrene | 6 | 540±57 | 26±3 | 780±59 | 110±21 |
Sodium azide | 1 | 54±6 | 39±4 | 101±69 | 481±7 |
Sodium azide | 5 | 43±6 | 35±6 | 395±21 | 896±23 |
Sodium azide | 10 | 36±12 | 46±13 | 786±130 | 986±50 |
Spontaneous revertants | - | 39±1 | 37±8 | 111±23 | 97±11 |
* Data are expressed as number of revertant colonies per plate per strain (means ± SD).
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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