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EC number: 268-071-2 | CAS number: 68002-58-4 This substance is identified by SDA Substance Name: C14-C18 dialkyl dimethyl ammonium methyl sulfate and SDA Reporting Number: 17-049-00.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- REPORTING FORMAT FOR THE ANALOGUE APPROACH
1. HYPOTHESIS FOR THE ANALOGUE APPROACH
This read-across is based on the hypothesis that source and target substances have similar toxicological properties because
• they are manufactured from similar or identical precursors under similar conditions
• they share structural similarities with common functional groups: quaternary ammonium and saturated or unsaturated alkyl chains with comparable length (corresponding to scenario 2 of the read-across assessment framework)
The read-across hypothesis is based on structural similarity of target and source substances. Based on available experimental data, including key physicochemical properties and data from acute toxicity, irritation, sensitization (human) and genotoxicity studies, the read-across strategy is supported by a quite similar toxicological profile of all substances.
Therefore, read-across from the existing ecotoxicity, environmental fate and toxicity studies conducted with the source substances is considered as an appropriate adaptation to the standard information requirements of the REACH Regulation for the target substance, in accordance with the provisions of Annex XI, 1.5 of the REACH Regulation.
A justification for read-across is attached to IUCLID section 13.
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
See justification for read-across attached to IUCLID section 13.
3. ANALOGUE APPROACH JUSTIFICATION
See justification for read-across attached to IUCLID section 13.
4. DATA MATRIX
See justification for read-across attached to IUCLID section 13. - Reason / purpose for cross-reference:
- read-across: supporting information
- Reason / purpose for cross-reference:
- read-across source
- Test organisms (species):
- other: Pseudomonas putida, or inoculum from a predominantly domestic wastewater treatment plant, or nitrifying bacteria
- Water media type:
- freshwater
- Duration:
- 18 h
- Dose descriptor:
- EC50
- Remarks:
- Pseudomonas putida, Bringmann & Kühn method
- Effect conc.:
- >= 48 - <= 58 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Remarks:
- Activated sludge
- Effect conc.:
- 520 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 119 h
- Dose descriptor:
- IC50
- Remarks:
- Nitrifying bacteria
- Effect conc.:
- 2.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of nitrification rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Remarks:
- Anaerobic bacteria from a domestic wastewater treatment plant
- Effect conc.:
- 220 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Conclusions:
- Nitrifying bacteria were found to be the most sensitive microorganisms with the lowest EC50 of 2.1 mg/L on which the risk assessment should be based.
Reference
Description of key information
EC50 of 2.1 mg/L (nitrifying bacteria; read across from DHTDMAC)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 2.1 mg/L
Additional information
No experimental data are available for the target substance Di-C14-18 alkyldimethyl ammonium methosulfate. However, toxicity studies in algae are available for the closely related source substance DHTDMAC. A justification for read-across is attached to IUCLID section 13.
Several studies are cited in the EU Risk Assessment Report on DODMAC (2002):
"The toxicity of DHTDMAC to Pseudomonas putida was investigated in a growth inhibition test according to a German DIN-guideline (Bringmann & Kühn method; UBA, 1992). In two tests EC50-values of 48 and 58 mg/l were derived after 18 hours (nominal values, graphically extrapolated).
In an activated sludge respiration inhibition test (OECD 209) inoculum from a predominantly domestic wastewater treatment plant was used (UBA, 1992). A 3h EC50 of 520 mg/l was derived graphically from the dose response curve. The corresponding statistically derived value was 267 mg/l (nominal concentrations).
The toxicity of DHTDMAC to nitrifying bacteria enriched in a laboratory wastewater treatment plant (domestic sludge originally) was investigated in a manometric respirometer test (Wagner & Kayser, 1990). The test duration in the reference was referred to between 119 and 254 hours for different substances and was stopped when the nitrification of the controls was completed. The IC50 for inhibition of respiration was 2.1 mg/l active ingredient of DHTDMAC (a carrier solvent was used).
Anaerobic bacteria from a domestic wastewater treatment plant were exposed to DHTDMAC in an OECD 209 test (Hoechst, 1989). The inhibition of respiration was measured after 3 hours and the EC10 was 80 mg/l, the EC50 = 220 mg/l."
Overall, nitrifying bacteria were found to be the most sensitive microorganisms with an EC50 of 2.1 mg/L on which the risk assessment should be based.
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