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EC number: 216-835-0 | CAS number: 1678-91-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1943
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- The physiological response of animals to cyclohexane, methylcyclohexane, and certain derivatives of these compounds. II Inhalation
- Author:
- Treon, J. et al.
- Year:
- 1 943
- Bibliographic source:
- J. Indust. Hygiene Toxicol. 25(8), 323-346
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Determination of the minimum lethal concentration after repeated inhalation exposure
- GLP compliance:
- not specified
- Test type:
- other: minimum lethal concentration
- Limit test:
- no
Test material
- Reference substance name:
- Methylcyclohexane
- EC Number:
- 203-624-3
- EC Name:
- Methylcyclohexane
- Cas Number:
- 108-87-2
- Molecular formula:
- C7H14
- IUPAC Name:
- methylcyclohexane
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Purity: 97%
Test animals
- Species:
- rabbit
- Strain:
- not specified
- Sex:
- not specified
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- air
- Details on inhalation exposure:
- exposure chamber:
higher concentrations: 223 l metal cage
long-term inhalation at lower concentrations: battery of nine insulated plywood cages, capacity 600 l.
temperature: 23.9 ± 1.7 °C
humidity: <45%
air flow rate: 350 to 800 liters/minute
vapour generator: Bosch fuel injection pump - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 6 h
- Remarks on duration:
- 6 hours per day
- Concentrations:
- 0.948, 1.46,4.57 mg/l: 300 hours
11.35 mg/l: 90 hours
21.90 mg/l: 120 hours
28.75, 39.55 mg/l: 60 hours
59.9 mg/L: 1 1/5 hours - No. of animals per sex per dose:
- 4
- Control animals:
- yes
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- not specified
- Dose descriptor:
- LC100
- Effect level:
- 59.9 mg/L air
- Based on:
- test mat.
- Exp. duration:
- 70 min
- Key result
- Sex:
- not specified
- Dose descriptor:
- other: mortality
- Effect level:
- 28.75 - < 39.55 mg/L air
- Based on:
- test mat.
- Exp. duration:
- 6 h
- Key result
- Sex:
- not specified
- Dose descriptor:
- other: minimum narcotic concentration
- Effect level:
- 21.9 mg/L air
- Based on:
- test mat.
- Exp. duration:
- 6 h
- Mortality:
- 0.948 - 21.90 mg/L: none
28.75 and 39.55 mg/L: mortality in 1/4 and 4/4 animals, respectively, after 2 weeks of exposure.
59.9 mg/L: mortality in 4/4 within 70 min after exposure initiation - Clinical signs:
- other: 0.948 - 11.35 mg/L: no effects 21.90 mg/L: slight lethargy. 28.75 mg/L: lethargy and impaired coordination of legs 39.55 mg/L: convulsions, light narcosis, laboured breathing, salivation, conjunctival congestion 59.55 mg/L: severe convulsions, rapid narco
- Body weight:
- Animals exposed to non-lethal concentrations gained weight. Mean group body weight gains were:
0.948 mg/L: 752 g (after 10 weeks)
4.57 mg/L: 956g (after 10 weeks)
11.35 mg/L: 328 g (after 3 weeks)
21.90 mg/L: 201 g (after 4 weeks)
Animals exposed to lethal concentrations lost weight. Mean group body weight gains were:
28.75 mg/L: -39 g (after 2 weeks)
39.55 mg/L: -390 g (after 2 weeks) - Gross pathology:
- Histopathology: After a 3-week exposure to 11.35 mg/L barely demonstrable evidence of cellular liver and kidney injury was reported.
Potential target organs: CNS at high concentrations
Applicant's summary and conclusion
- Interpretation of results:
- other: STOT-SE 3 H336 May cause drowsiness and dizziness
- Conclusions:
- In this rabbit study, all the animals died at 59.9 mg/L within 70 min after exposure initiation. LC50 was considered as between 39.6 and 59.9 mg/L. Signs of toxicity at 59.9 mg/L (70 min exposure) included severe convulsions, rapid narcosis, labored breathing, salivation, and conjunctival congestion.
- Executive summary:
In this rabbit study, all the animals died at 59.9 mg/L within 70 min after exposure initiation. LC50 was considered as between 39.6 and 59.9 mg/L. Signs of toxicity at 59.9 mg/L (70 min exposure) included severe convulsions, rapid narcosis, labored breathing, salivation, and conjunctival congestion.
In this study, mortality after single and repeated exposure occurred only at high to very high concentrations of methylcyclohexane, which are above the currently valid LC50 ranges used for classification. Therefore, the study results do not fulfil the classification criteria for acute toxicity by inhalation. Based on the narcotic effects consistently observed at high concentrations in this and further studies (Weight of Evidence), methylcyclohexane fulfils the classification criteria for Specific target organ toxicity-single exposure (STOT-SE) Category 3 (narcotic effects).
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