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EC number: 619-220-9 | CAS number: 96499-68-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 Jan - 01 Feb 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- April 13, 2004
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Dodecanoic acid, monoester with oxybis[propanediol]
- EC Number:
- 619-220-9
- Cas Number:
- 96499-68-2
- Molecular formula:
- not applicable, the substance is UVCB
- IUPAC Name:
- Dodecanoic acid, monoester with oxybis[propanediol]
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all concentration levels and control
- Sampling method: Approx. 60 mL of test solution was sampled from the middle layer of each test vessel. Sampling was performed at the start of exposure, before and after the renewal at 24 h and at the end of exposure.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test sample and dilution water were mixed to produce a nominal concentration of 80.0 mg/L and stirred for 48 hours. The suspension was filtered with a membrane filter (0.22 µm pore size) to prepare the stock solution. The test solutions were prepared by diluting the stock solution with dilution water.
- Eluate: no
- Differential loading: no
- Controls: blank control, reference control
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): After filtration, the stock solution was clear.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: The University of Sheffield, United Kingdom; supplied on July 9, 1990
- Age of parental stock: 20 d
- Feeding during test: no
- Breeding conditions of parental stock: breeding in dechlorinated tap water at 20 ± 1 °C; photoperiod: 16 h light/8 h darkness; survival of parental stock: 100%; during breeding, the daphnids were fed Chlorella vulgaris once a day (corresponding to 0.1-0.2 mg organic carbon per day)
- Age of daphnids for test: less than 24 h
ACCLIMATION
- Acclimation period: not specified
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 43 mg/L (Ca, Mg)
- Test temperature:
- 20.0 - 20.1 °C
- pH:
- Control: 7.7 - 7.9
Treatment: 7.8 - 8.0 - Dissolved oxygen:
- Control: 7.9 - 9.0 mg/L
Treatment: 7.6 - 9.0 mg/L - Conductivity:
- 17 mS/m
- Nominal and measured concentrations:
- nominal: Control, 0.316, 1.00, 3.16, 10.0, 31.6 and 100 mg/L
measured (geometric mean): < detection limit, 0.215, 0.681, 2.27, 7.48, 23.7 and 77.4 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL glass beaker, covered with transparent plastic lid
- Renewal rate of test solution: fresh test solutions were prepared after 24 h
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Total organic carbon: < 0.5 mg/L
- Particulate matter: < 1 mg/L
- Chlorine: < 0.02 mg/L
- Alkalinity: 42 mg/L
- Ca/mg ratio: 3.14
- Conductivity: 17 mS/m
- Culture medium different from test medium: same as test medium
- Intervals of water quality measurement: pH value, temperature and dissolved oxygen concentration were measured at test start, before and after the renewal at 24 h and at test end
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light/8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility and behavioural effects were observed after 24 and 48 h
RANGE-FINDING STUDY
Two preliminary tests were performed.
Test 1:
- Concentrations: 1.00, 5.00, 10.0 mg/L (nominal)
- Results used to determine the conditions for the definitive study: After 48 h, 0% immobility was observed at the two lowest concentration levels, while 90% immobilisation was observed at the highest concentration level.
Test 2:
- Concentrations: 1.00, 10.0, 100 mg/L (nominal)
- Results used to determine the conditions for the definitive study: After 48 h, 0% immobility was observed at the lowest concentration levels, while 70% immobilisation was observed at the intermediate concentration level and 100% immobilisation at the highest concentration level. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9.69 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI could not be determined
- Details on results:
- - Behavioural abnormalities: After 48 h, one daphnid in the highest test concentration showed reduced activity.
- Mortality of control: 0%
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No, the test solutions were clear and colourless at test start and end. - Results with reference substance (positive control):
- - Results with reference substance valid?
Yes
- EC50 (48 h) = 0.12 mg/L - Reported statistics and error estimates:
- The 48 hour EC50 was calculated by Binomial test. For 24 hours, the EC50 was estimated as ">the highest test concentration" since immobility over 50% was not observed. In 0.316% exposure level, the test item concentrations were less than the determination limit throughout exposure duration. Therefore the test item concentration was represented as reference values which were calculated from a geometric series with a factor √10 and the measured concentration of the quantifiable lowest exposure level (1.00% exposure level).
Any other information on results incl. tables
Table 1: Immobility
Measured concentration [mg/L] |
Vessel |
24 hours |
48 hours |
||
No. of immobilied daphnids/Total daphnids |
Immobility [%] |
No. of immobilied daphnids/Total daphnids |
Immobility |
||
Control |
A |
0/5 |
0 |
0/5 |
0 |
B |
0/5 |
0/5 |
|||
C |
0/5 |
0/5 |
|||
D |
0/5 |
0/5 |
|||
0.215 |
A |
0/5 |
0 |
0/5 |
0 |
B |
0/5 |
0/5 |
|||
C |
0/5 |
0/5 |
|||
D |
0/5 |
0/5 |
|||
0.681 |
A |
0/5 |
0 |
0/5 |
0 |
B |
0/5 |
0/5 |
|||
C |
0/5 |
0/5 |
|||
D |
0/5 |
0/5 |
|||
2.27 |
A |
0/5 |
0 |
0/5 |
0 |
B |
0/5 |
0/5 |
|||
C |
0/5 |
0/5 |
|||
D |
0/5 |
0/5 |
|||
7.48 |
A |
0/5 |
0 |
2/5 |
30 |
B |
0/5 |
1/5 |
|||
C |
0/5 |
2/5 |
|||
D |
0/5 |
1/5 |
|||
23.7 |
A |
5/5 |
55 |
5/5 |
100 |
B |
3/5 |
5/5 |
|||
C |
2/5 |
5/5 |
|||
D |
1/5 |
5/5 |
|||
77.4 |
A |
1/5 |
25 |
5/5 |
75 |
B |
2/5 |
4/5 |
|||
C |
2/5 |
3/5 |
|||
D |
0/5 |
3/5 |
Table 2: Validity criteria
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
0% |
yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
≥7.6 mg/L |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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