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Diss Factsheets
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EC number: 947-623-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biotransformation and kinetics
Administrative data
- Endpoint:
- biotransformation and kinetics
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well documented study according to a sound study design.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The potential for biotransformation of TSP by hepatic enzymes was investigated in rainbow trout liver S9 fractions. Rainbow trout liver S9 fractions were incubated with TSP for 120 min. In addition, buffer-only, heat deactivated and No NADPH controls were assayed alongside the S9 reaction mix fractions. Over the 120 min, time course samples were taken and analyzed by GC-MS to determine the loss of TSP relative to the control samples.
- GLP compliance:
- yes
- Type of medium:
- animal
Test material
- Reference substance name:
- 2,4,6-tris(1-phenylethyl)phenol
- EC Number:
- 242-128-1
- EC Name:
- 2,4,6-tris(1-phenylethyl)phenol
- Cas Number:
- 18254-13-2
- Molecular formula:
- C30H30O
- IUPAC Name:
- 2,4,6-tris(1-phenylethyl)phenol
- Test material form:
- liquid: viscous
- Details on test material:
- The test material is one of the main constituents of the reaction mass of 2,4,6-tris(1-phenylethyl)phenol and Bis(1-phenylethyl) phenol.
Constituent 1
Results and discussion
- Transformation products:
- no
Any other information on results incl. tables
The liver S9 assay was conducted with the assays being stopped using 1:1 ACN:THF. The results of the biotransformation assay of 5 µM TSPafter 120 min of incubation in the rainbow trout S9 fractionare compiled in the below table.
At 120 minutes, no loss of TSP wasobserved relative to Time 0 min. Likewise, the results indicated that there was no loss wit the heat-inactivated liver S9 fraction and No-NADPH negative controls. The positive controldisappeared to levels below analytical detection before 120 mins of incubation exhibited. Overall, the data indicates there is no metabolic degradation of TSP using the in vitro liver S9 metabolism assay.
It should be noted that higher levels of TSP and fluroxypyr were detected in this S9 study, as opposed tothe subsequent hepatocyte experiments, even though both studies utilized the same concentration . While adefinitive reason cannot be given, it is likely due to differences in binding within the cell suspension and ultimate bioavailability. It is likely that these compounds had a greater affinity for the hepatocytes compared to the S9 suspension, thus lowering the overall bioavailable free measurable fraction.
|
|
TSP S9 Reaction Mix |
No NADPH Control |
Heat Inactivated Control |
Positive Control |
Run |
Time point |
TSP Conc. (ppm) |
TSP Conc. (ppm) |
TSP Conc. (ppm) |
TSP Conc. (ppm) |
1 |
0 min |
27.6 |
# |
20.78 |
17.03 |
2 |
0 min |
21.0 |
22.50 |
20.24 |
17.50 |
3 |
0 min |
23.6 |
21.50 |
21.07 |
17.73 |
Avg. |
0 min |
24.1 |
21.82 |
20.70 |
17.38 |
1 |
15 min |
21.4 |
|
|
|
2 |
15 min |
33.8 |
|
|
|
3 |
15 min |
27.1 |
|
|
|
Avg. |
15 min |
27.4 |
|
|
|
1 |
30 min |
33.8 |
|
|
|
2 |
30 min |
29.7 |
|
|
|
3 |
30 min |
26.7 |
|
|
|
Avg. |
30 min |
30.0 |
|
|
|
1 |
60 min |
21.8 |
# |
20.36 |
BD |
2 |
60 min |
29.5 |
22.48 |
19.72 |
BD |
3 |
60 min |
26.4 |
25.19 |
20.28 |
BD |
Avg. |
60 min |
25.9 |
23.66 |
20.12 |
-0.42 |
1 |
120 min |
21.7 |
# |
17.61 |
BD |
2 |
120 min |
23.3 |
22.27 |
22.56 |
BD |
3 |
120 min |
30.7 |
21.29 |
20.55 |
BD |
Avg. |
120 min |
25.2 |
21.69 |
20.24 |
-0.42 |
# Vial broken, could not be analyzed.
Applicant's summary and conclusion
- Conclusions:
- There was not significant loss of parent materials over time due to the active biotransformation of liver S9 from rainbow trout.
- Executive summary:
The potential for biotransformation of TSP by hepatic enzymes was investigated in rainbow trout liver S9 fractions. Rainbow trout liver S9 fractions were incubated with TSP for 120 min. In addition, buffer-only, heat deactivated and No NADPH controls were assayed alongside the S9 reaction mix fractions. Over the 120 min, time course samples were taken and analyzed by GC-MS to determine the loss of TSP relative to the control samples.
5 µM of TSP was incubated with rainbow trout liver S9 fractions (1 mg/ml protein) for 120 min. One-way ANOVA followed by Dunnett's post hoc testing showed no significant loss of TSP relative to time 0 minutes for the liver S9 assays (p > 0.05). No significant differences were observed for loss of TSP in the Heat Deactivated Controls for the liver S9 assay.
In conclusion there was not significant loss of parent materials over time due to the active biotransformation of liver S9 from rainbow trout.
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