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EC number: 911-418-6 | CAS number: 55965-84-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Test material form:
- other: orange-yellow liquid
- Details on test material:
- - Name of test material (as cited in study report): Kathon™ WT
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Rohm and Haas, Batch No. J 97030
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature
- Stability under test conditions: Stable at room temperature
- Solubility and stability of the test substance in the solvent/vehicle: Soluble and stable
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: No
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Dilution in water
- Final dilution of a dissolved solid, stock liquid or gel: 0.0010, 0.010, 0.10, 1.0 and 10 mg whole material/L
OTHER SPECIFICS: Purity of test material was 14%
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Dispersion
- Controls: Negative control and positive control (3,5-dichlorophenol)
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): No
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: Municipal wastewater treatment plant in Newburyport, Massachusetts, USA which treats predominantly domestic waste.
- Method of cultivation: Not applicable
- Preparation of inoculum for exposure: Aeration
- Pretreatment: Incubated at room temperature
- Initial biomass concentration: Total suspended solids = 9.7 g/L on day of collection and 4.2 g/L on the day of the test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- 10 min
Test conditions
- Hardness:
- Not described
- Test temperature:
- 20 +/- 2°C
- pH:
- 7.9 at test initiation
- Dissolved oxygen:
- 7.9-9.6 mg/L
- Salinity:
- Not applicable
- Conductivity:
- Not described
- Nominal and measured concentrations:
- Nominal: 0 (control), 6.5, 13, 25, 50, and 100 mg/L test substance
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Static
- Material, size, headspace, fill volume: 1 L glass beaker, 500 mL headspace
- Aeration: 0.5 to 1.0 L air/min
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Sludge concentration (weight of dry solids per volume): 4.2 g/L
- Nutrients provided for bacteria: 16 mL synthetic sewage per vessel
- Nitrification inhibitor used (delete if not applicable): none
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Synthetic sludge
OTHER TEST CONDITIONS
- Adjustment of pH: adjusted to 7.5 with 0.1 N HCl
- Photoperiod: all light
- Light intensity: ambient lighting, 5 µEin/m^2s
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Dissolved oxygen measurement for first 10 minutes that sludge was in the BOD bottle.
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 0.0010, 0.010, 0.10, 1.0, and 10 mg/L
- Results used to determine the conditions for the definitive study: Yes - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.91 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No
- Effect concentrations exceeding solubility of substance in test medium: No
- Adsorption (e.g. of test material to the walls of the test container): Not described
- Blank controls oxygen uptake rate: 60 mg/L/h
- Coefficient of variation of oxygen uptake rate in control replicates: Not described - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50 = 9.6 mg/L - Reported statistics and error estimates:
- The respiration rate was calculated as the mg oxygen/liter/hour consumed between 6.5 and 2.5 mg/L or over a 10 minute respiration period if the respiration rate was low. The percent inhibition, which was used to calculate EC50 values, was calculated as 1 minus ([2 times the respiration rate of the TS concentration] divided by the sum of the 2 control respiration rates) times 100.
The probit method was used to calculate the reference toxicant 3-h EC50 using nominal concentrations (Stephan, 1983, Computer Program for Calculation of LC50 values, personal communication).
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the results, the 3h EC50 for CMIT/MIT based on inhibition of respiration was determined to be 4.5 mg/L. The 3h NOEC was 0.91 mg/L.
- Executive summary:
Exposure of activated sludge to Kathon(TM) WT 14% resulted in a 3 hour EC50 of 4.5 mg/L with a 95% confidence interval of 3.9 to 5.2 mg/L, on an active ingredient basis. The 3 hour NOEC is 0.91 mg/L on an active ingredient basis. Both parameters are based on inhibition of respiration after a 3 h exposure to the test material.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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