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EC number: 679-769-5 | CAS number: 2675-94-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 1993-05-24 to 1993-06-14
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)
- Version / remarks:
- 1984
- GLP compliance:
- yes
Test material
- Reference substance name:
- N,N-diethylprop-2-enamide
- EC Number:
- 679-769-5
- Cas Number:
- 2675-94-7
- Molecular formula:
- C7-H13-N-O
- IUPAC Name:
- N,N-diethylprop-2-enamide
Constituent 1
- Specific details on test material used for the study:
- Batch No.: not specified
Purity: not specified
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: England
- Age at study initiation: approximately 14 weeks age
- Weight at study initiation: 3334g
- Housing: be housed individually in a plastic cage with a perforated floor
- Diet: ad libitum
- Water: ad libitum
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 19
- Humidity (%): 30 to 70
- Air changes (per hr): 19
- Photoperiod (hrs dark / hrs light): 12 hours artificial light in each 24 hours period
Test system
- Vehicle:
- unchanged (no vehicle)
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied: 0.1mL - Duration of treatment / exposure:
- 24 hours
- Observation period (in vivo):
- 21 days
- Number of animals or in vitro replicates:
- 1
- Details on study design:
- The eye were examined prior to instillation of the test substance to ensure that there was no pre-existing corneal damage, iridial or conjunctival inflammation.
One animal was treated in advance of the others, to ensure that if severe response was produced. No further animals would be exposed.
A 0.1 mL amount of the test substance was placed into the everted lid of one eye.
The eyelids were then gently held together for one second before releasing. The contralateral eye reminded untreated.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 1
- Reversibility:
- fully reversible within: 21 days
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 1.3
- Max. score:
- 2
- Reversibility:
- fully reversible within: 7 days
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 2.3
- Max. score:
- 3
- Reversibility:
- fully reversible within: 21 days
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 2
- Max. score:
- 2
- Reversibility:
- fully reversible within: 21 days
- Irritant / corrosive response data:
- A corneal opacity developed one day after instillation. (Dulling and stripping of the epithelial cells were seen one hour after installation).
Iridial inflammation was observed one hour after instillation and 24 hours after installation has developed to Grade 2.
A beefy red colouration of the conjunctive accompanied by considerable swelling with partial eversion of the eyelids was observed. Blanching was observed in the nictating membrane.
Due to the severity of the reaction no further animals were exposure to the test item.
Any other information on results incl. tables
Ocular reations observed after instillation of the test item
rabbit number and sex | region of eye | 1 hour | day after installation | |||||||
1 | 2 | 3 | 4 | 7 | 14 | 21 | ||||
1849 male* | cornea | DA | 1 | 1 | 1 | 1 | 1 | 1 | 0 | |
iris | 1 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | ||
conjunctiva | redness | B2 | B2 | B2 | B3 | B3 | B2 | B2 | 0 | |
chemosis | 2 | 2 | 2 | 2 | 2 | 1 | 1 | 0 |
* pilot animal
A stripping of the nictating
B blanching on the nictating membrane
D dulling of the normal lustre of the cornea
Applicant's summary and conclusion
- Conclusions:
- Instillation of test item into rabbit eye elicited severe ocular lesions.
Due to the severity of the reaction no further animals were exposure to the test item. - Executive summary:
The study was performed to assess the eye irritation potential of the rabbit. The method followed was in EEC methods for the determination of toxicity, Directive 84/449/EEC, part B, Method B.5. Acute toxicity (eye irritation).
One rabbit was administered a single ocular dose of 0.1 mL of the test item and observed for 21 days after instillation.
A single instillation of the test item into eye of the rabbit elicited severe ocular lesions.
In view of the severity of the ocular reactions seen no further testing was performed.
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