Registration Dossier
Registration Dossier
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EC number: 280-116-8 | CAS number: 83006-67-1
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Nov 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
- Report date:
- 1979
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: DIN 380412 L15 (Fish, Acute toxicity test) o 1976
- GLP compliance:
- no
Test material
- Reference substance name:
- Disodium 2,2'-[(9,10-dihydro-5,8-dihydroxy-9,10-dioxo-1,4-anthrylene)diimino]bis[5-tert-butylbenzenesulphonate]
- EC Number:
- 280-116-8
- EC Name:
- Disodium 2,2'-[(9,10-dihydro-5,8-dihydroxy-9,10-dioxo-1,4-anthrylene)diimino]bis[5-tert-butylbenzenesulphonate]
- Cas Number:
- 83006-67-1
- Molecular formula:
- C34H34N2O10S2.2Na
- IUPAC Name:
- Disodium 2,2'-[(9,10-dihydro-5,8-dihydroxy-9,10-dioxo-1,4-anthrylene)diimino]bis[5-tert-butylbenzenesulphonate]
- Test material form:
- solid
- Details on test material:
- Acid Green 81
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Leuciscus idus melanotus
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Nominal and measured concentrations:
- 100, 200 mg/L
- Details on test conditions:
- TEST SYSTEM
- No. of organisms per vessel: 2
- No. of vessels per concentration (replicates): 1 - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 141 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
Any other information on results incl. tables
- Sublethal observations / clinical signs:
Nominal concentration
[mg/L]number of fish with adverse symptoms number of dead fish after 3h after 24h after 72h after 3h after 72h 100 0 0 0 0 0 200 2 - - 2 -
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 72-h LC50 to Leuciscus idus was calculated to be 141 mg/L. The substance is not acutely toxic to fish. No dead animals found at 100 mg/L.
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