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Reaction mass of hydrogen [1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)][1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1) and hydrogen bis[1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1) and hydrogen bis[1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1)
EC number: 916-881-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 (Acute Toxicity (Oral))
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Reaction mass of hydrogen [1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)][1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1) and hydrogen bis[1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1) and hydrogen bis[1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1)
- IUPAC Name:
- Reaction mass of hydrogen [1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)][1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1) and hydrogen bis[1-[(2-hydroxy-4-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1) and hydrogen bis[1-[(2-hydroxy-5-nitrophenyl)azo]-2-naphtholato(2-)]chromate(1-) , compound with 3-[(2-ethylhexyl)oxy]propylamine (1:1)
- Test material form:
- solid: particulate/powder
- Details on test material:
- - Description: Black powder
- Storage Conditions: Room temperature
Constituent 1
- Specific details on test material used for the study:
- TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: The test material was prepared at a concentration of 50 % w/v in corn oil and administered at a volume of 10.0 mL/kg. The test material was prepared on the day of dosing.
Test animals
- Species:
- rat
- Strain:
- other: Crl:CD (SD) BR VAF plus
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: Approximately four to six weeks of age.
- Weight at study initiation: Weight range of 100 to 123 g prior to dosing.
- Fasting period before study: Yes. Access to food only was prevented overnight prior to and approximately 4 hours after dosing.
- Housing: Housed in groups of up to five rats of the same sex in metal cages with wire mesh floors.
- Diet: Ad libitum
- Water: Ad libitum
- Acclimation period: All the rats were acclimated to the experimental environment for a period of 6 days prior to the start of the study.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 °C to 25 °C
- Humidity (%): 60 % R.H
- Air changes (per hr): The rate of air exchange was maintained at approximately 15 air changes/hour.
- Photoperiod (hrs dark / hrs light): Lighting was controlled by means of a time switch to provide 12 hours artificial light in each 24-hour period.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: The test material was prepared at a concentration of 50 % w/v in corn oil.
MAXIMUM DOSE VOLUME APPLIED:
The test material was administered at a volume of 10.0 mLkg. - Doses:
- 5.0 g/kg bodyweight. The appropriate dose volume of the test substance was administered to each rat using a syringe and plastic catheter (10 choke).
- No. of animals per sex per dose:
- Five males and five females.
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: All animals were observed for 14 days after dosing. The day of dosing was designated Day 1.
- Frequency of observations and weighing: Animals were observed soon after dosing and at frequent intervals for the remainder of Day 1 (a period of five hours). On subsequent days the animals were observed once in the morning and again at the end of the experimental day. Clinical signs were recorded at each observation. The nature, severity, approximate time of onset and duration of each toxic sign.
Individual bodyweights of rats were recorded on Days 1 (day of dosing), 8 and 15.
- Necropsy of survivors performed: Yes. All animals were killed on Day 15 by cervical dislocation and were subjected to a macroscopic post mortem examination which consisted of opening the abdominal and thoracic cavities. The macrospic appearance of all examined tissues were recorded.
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- There were no deaths following a single oral dose of test material at 5.0 g/kg bodyweight.
- Clinical signs:
- other: Pilo-erection was observed in all rats within five minutes of dosing and throughout the remainder of Day 1. Dark blue eyes and extremities were observed in all rats from Day 3 to Day 6 or 7. Abnormal body carriage (hunched posture) was also observed in al
- Gross pathology:
- Terminal autopsy findings were normal.
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified in accordance with EU criteria.
- Conclusions:
- Under the conditions of the study, the acute lethal oral dose was greater than 5 000 mg/kg bodyweight in male and female rats.
- Executive summary:
The acute oral toxicity of the test material was assessed in the rat in accordance with the standardised guideline EU Method B.1 under GLP conditions.
A group of ten rats (five males and five females) was treated with test material at 5 000 mg/kg bodyweight. The appropriate dose volume of the test substance was administered to each rat using a syringe and plastic catheter (10 choke). All animals were observed for clinical signs and mortality for a period of 14 days after dosing. Individual bodyweight gains were also monitored. All animals were euthanised on Day 15 by cervical dislocation and were subjected to a macroscopic post mortem examination.
There were no deaths following a single oral dose of test material at 5 000 mg/kg bodyweight. Pilo-erection was observed in all rats within five minutes of dosing and throughout the remainder of Day 1. Dark blue eyes and extremities were observed in all rats from Day 3 to Day 6 or 7. Abnormal body carriage (hunched posture) was also observed in all females during this period. Recovery, as judged by external appearance and behaviour, was complete by Day 9. Slightly low bodyweight gains during the first week of the study were recorded for one male and three female rats on Day 8. All other rats achieved anticipated bodyweight gains throughout the study. Terminal autopsy findings were normal.
Under the conditions of the study, the acute lethal oral dose to rats of the test material was found to be greater than 5 000 mg/kg bodyweight.
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