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EC number: 283-640-5 | CAS number: 84696-21-9 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Hydrocotyle asiatica, Umbelliferae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Adsorption / desorption
Administrative data
Link to relevant study record(s)
- Endpoint:
- adsorption / desorption: screening
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- February 21 to 24, 2020
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- HPLC estimation method
- Specific details on test material used for the study:
- Centella asiatica dry extract
Batch No. 972/43/A
Appearance dark green, powder
Retesting date: August 31, 2020
Storage: room temperature - Radiolabelling:
- not specified
- Test temperature:
- 25 °C
- Details on study design: HPLC method:
- Detector: 200 nm (for the reference items)
Column: LiChrospher 100 CN (5µm) 250-4, No.: 933278
Temperature: 25 °C
Mobile Phase: Acetonitrile : Water = 3 : 7 (v/v)
Flow Rate: 1 mL/min (for the reference items)
Injection Volume: 20 µL (2 µL for Acetanilide)
Test Solutions
The reference items were dissolved in acetonitrile. The concentrations of the stock solutions were about 1 mg/mL. These stock solutions were diluted with the mobile phase resulting in concentrations of approximately 10 µg/mL.
The test item was dissolved in acetonitrile. The concentration of the stock solution was about 1 mg/mL. This stock solution was diluted with the mobile phase resulting in concentration of approximately 10 µg/mL
Test Solutions
The reference items were dissolved in acetonitrile. The concentrations of the stock solutions were about 1 mg/mL. These stock solutions were diluted with the mobile phase resulting in concentrations of approximately 10 µg/mL.
The test item was dissolved in acetonitrile. The concentration of the stock solution was about 1 mg/mL. This stock solution was diluted with the mobile phase resulting in concentration of approximately 10 µg/mL.
Three series of the above-mentioned reference solutions were measured with two parallel injections, each. Three series of the test item solution were measured with two parallel injections, each. The retention times were determined. The three calibration series and three test item series were measured alternately.
For the determination of t0 (t0 = retention time of the unretarded component) three series of sodium nitrate solution were injected twice.
The pH of the mobile phase was 5.67.
Madecassicoside and Asiaticoside component of the test item was detected by MS, the Acetanilide reference item, as a first eluting standard was detected by UV and MS method, too. The difference of retention times was 0.023 min. The retention times of Madecassicoside and Asiaticoside component of the test item was corrected with this value.
Results Evaluation
The capacity factors, k were calculated from the retention times (tR) of the selected reference items and the dead time (t0):
Then the log k data of the reference items were plotted against their log Koc values given in Table 2.
The regression coefficients (a, b) were determined by linear regression calculation using the least squares method.
The log Koc value of a test item is calculated, using these regression coefficients and the capacity factor of the test item:
log k = a + b*log Koc
where:
Koc adsorption coefficient
a constant
b slope
k capacity factor - Type:
- log Koc
- Value:
- < 1.25 dimensionless
- Temp.:
- 25 °C
- Details on results (HPLC method):
- Retention times measured for Madecassicoside and Asiaticoside component of the test item (3.072 and 3.408 min) are lower than the retention time of the first eluting standard: Acetanilide (4.195 min). This result shows that log Koc of Madecassicoside and Asiaticoside component of the test item is lower than the calibrated range. As Acetanilide is the reference item with the lowest Koc value (OECD 121).
- Validity criteria fulfilled:
- yes
- Conclusions:
- Using the HPLC method the log Koc of Madecassicoside and Asiaticoside component of the test item was determined to be < 1.25.
Reference
Description of key information
Using the HPLC method the log Koc of Madecassicoside and Asiaticoside component of the test item was determined to be < 1.25.
Key value for chemical safety assessment
- Koc at 20 °C:
- 17.78
Additional information
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