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EC number: 269-304-0 | CAS number: 68214-62-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Study period:
- January 22nd to March 22d, 1991
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Justification for type of information:
- The read across approach is detailed into the document attached to the IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: laboratory culture originating from a strain supplied by I.R.CH.A., France.
- Breeding: testing laboratoyr, cultures maintained at 21°C in polypropylene vessels containing two litres of dechlorinated and aged tap water. Culture conditions ensure that reproduction is by part
henogenesis.
- Age of parental stock: gravid adults were isolated 24 hours prior to initiation of the test. Young daphnids produced overnight were used for testing.
- Feed: cultures were fed daily with a suspension of mixed algae (predominantly Chlorella spp.). - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- Start: 21 °C
End: 21 °C - pH:
- Start: 7.4
End: 7.2 - Dissolved oxygen:
- Start: 8.7 mg O2/l
Start: 8.1 mg O2/l - Nominal and measured concentrations:
- 5.0, 9.0, 16, 28, 50, 90, 160, 280 and 500 mg active ingredient/l
- Details on test conditions:
- TEST SYSTEM
- Daphnia loading: according to test protocol.
TEST MEDIUM / WATER PARAMETERS
- Water quality characteristics: according to test protocol. - Reference substance (positive control):
- yes
- Remarks:
- K2Cr2O7
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 140 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Details on results:
- EC0 (48h): 16 mg a.i./l (nominal)
EC100 (48h): 500 mg a.i./l (nominal) - Results with reference substance (positive control):
- EC50 (24h): 1.5 mg/l
- Conclusions:
- EC50 (48h): 140 mg a.i./l (nominal)
- Executive summary:
The acute toxicity of substance to Daphnia magna was investigated under static exposure conditions over a period of 48 hours, according to OECD guideline 202. The following concentrations were tested: 5.0, 9.0, 16, 28, 50, 90, 160, 280 and 500 mg active ingredient/l.
Potassium dichromate was also tested as reference substance.
The maximum concentration causing no immobilization after 48 hours was 16 mg a.i./l (nominal); on the contrary, at the concentration of 500 mg a.i./l all the test organisms resulted to be immobilized. The EC50 at 48 hours was determined to be 140 mg a.i./l.
Conclusion
EC50 (48h): 140 mg a.i./l (nominal)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Study period:
- From April 06th to September 27th, 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Justification for type of information:
- The read across approach is detailed into the document attached to the IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- part I, adopted 1984
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Version / remarks:
- September 19, 1984
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Test item was dissolved in the test medium at a concentration of 100 mg/100 ml (stock solution). Thereafter, series of sequential dilutions with the test medium were prepared to obtain the final test concentrations of the test article.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Animals were bred in the testing laboratory, under standardized conditions.
For the test, animals with an age of less than 24 hours were used. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Test temperature:
- 21.5 °C
- pH:
- Start: 8.4
End: 8.3 - Dissolved oxygen:
- Start: 8.6 mg/l
End: 9.0 mg/l - Nominal and measured concentrations:
- Nominal 0, 1, 10 and 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml beakers.
- Volume: beakers contain 20 ml of test medium.
- No. of organisms per vessel: 10 animals.
- No. of vessels per concentration: 2 replicates.
- No. of vessels per control: 2 replicates.
TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: bidistilled water.
- Test medium: according to the Directive EEC.
- CaCl2 × 2H2O: 294 mg/l
- MgSO4 × 7H2O: 123 mg/l
- NaHCO3: 65 mg/l
- KCl: 5.8 mg/l.
OTHER TEST CONDITIONS
- Adjustment of pH:pH of reconstituted water was adjusted to 7.9 ± 0.3.
- Photoperiod: 16 hours of illumination.
- Light intensity: 1000 Lux.
EFFECT PARAMETERS MEASURED
Test evaluations were done according to the OECD guideline 202 by visual control of the mobility of the daphnids after 24 hours. Based on the data, an estimate of the EC0, 50 and 100 was given. - Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- No deaths occurred over the exposure period of 24 hours. EC0 (24h): 100 mg/l
Appearance of the test medium: blue solution. - Conclusions:
- EC50 (24h) > 100 mg/l (nominal)
- Executive summary:
The acute toxicity of the test substance to Daphnia magna was investigated over a period of 24 hours, following the OECD guideline 202, part I (1984). The nominal test concentrations applied were 0, 1, 10 and 100 mg/l; all tests were run in duplicate. No chemical analysis of the test substance concentration was conducted. No deaths occurred at the nominal concentration of 100 mg/l; 10 % of mortality was observed in one of the two blank controls. The EC0 was identified to be 100 mg/l. Test medium appeared to be blue solution.
Conclusion
EC50 (24h) > 100 mg/l (nominal)
Referenceopen allclose all
Cumulative immobilisation of daphnids (%) test item
Concentration (mg a.i./l) | 24 hrs | 48 hrs |
Control | 0 | 0 |
Test item 5.0 | 0 | 0 |
Test item 9.0 | 0 | 0 |
Test item 16 | 0 | 0 |
Test item 28 | 5 | 5 |
Test item 50 | 5 | 15 |
Test item 90 | 10 | 20 |
Test item 160 | 20 | 60 |
Test item 280 | 30 | 70 |
Test item 500 | 60 | 100 |
Immobilisation rate of daphnids (%) reference substance
Concentration (mg a.i./l) | 24 hrs | 48 hrs |
Control | 0 | 0 |
Reference substance 0.10 | 0 | 0 |
Reference substance 0.18 | 0 | 0 |
Reference substance 0.32 | 0 | 0 |
Reference substance 0.56 | 5 | 5 |
Reference substance 1.0 | 5 | 15 |
Reference substance 1.8 | 10 | 20 |
Reference substance 3.2 | 20 | 60 |
Reference substance 5.6 | 30 | 70 |
Reference substance 10 | 60 | 100 |
Influence of test item on the immobilization of Daphnia magna, after 24 hours of exposure
Concentration (mg/l) | N. of daphnids | N. of responses | % of responses |
Control | 10 | 1 | 10 |
Control | 10 | 0 | 0 |
Test item 1 | 10 | 0 | 0 |
Test item 1 | 10 | 0 | 0 |
Test item 10 | 10 | 0 | 0 |
Test item 10 | 10 | 0 | 0 |
Test item 100 | 10 | 0 | 0 |
Test item 100 | 10 | 0 | 0 |
Description of key information
EC50 (48h) > 100 mg/l
Key value for chemical safety assessment
Additional information
There are no data about the short-term toxicity potential of Acid Blue 280 to aquatic invertebrates; therefore, available data on structural analogues Similar Substance 01 and Similar Substance 02 has been taken into consideration. The read across approach can be considered as appropriate and suitable to assess the property under investigation (details about the approach are reported into the IUCLID section 13).
As both studies were reliable, the assessment was based on a weight of evidence approach.
The acute toxicity of the Similar Substance 01 to Daphnia magna was investigated over a period of 24 hours, following OECD guideline 202, part I (1984). The nominal test concentrations applied were 0, 1, 10 and 100 mg/l; all tests were run in duplicate. No chemical analysis of the test substance concentration was conducted. No deaths occurred at the nominal concentration of 100 mg/l; 10 % of mortality was observed in one of the two blank controls. The EC0 was identified to be 100 mg/l. Test medium appeared to be a blue solution.
The acute toxicity of Similar Substance 02 to Daphnia magna was investigated under static exposure conditions over a period of 48 hours, according to OECD guideline 202. The following concentrations were tested: 5.0, 9.0, 16, 28, 50, 90, 160, 280 and 500 mg active ingredient/l. The maximum concentration causing no immobilization after 48 hours was 16 mg a.i./l (nominal); on the contrary, at the concentration of 500 mg a.i./l all the test organisms resulted to be immobilized. The EC50 at 48 hours was determined to be 140 mg a.i./l.
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