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EC number: 685-540-0 | CAS number: 208126-52-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 Sept. 2007 - 11 Jan. 2008
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 7-2-A
- Expiration date of the lot/batch: April 2010
- Purity test date: not stated
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature at about 20 °C, away from direct sunlight
- Stability under test conditions: not stated
- Solubility and stability of the test substance in the solvent/vehicle: not stated
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not stated
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: The test item is a complex mixture containing different sparingly soluble compounds. In order to assess its toxicity, water accomodated fractions (WAF's) were prepared.
- Preliminary purification step (if any): none
- Final dilution of a dissolved solid, stock liquid or gel:
The following WAFs with the loading rates of 0.32, 1.0, 3.2, 10, 32 and 100 mg/l were prepared. - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: from each test concentration
- Sampling method: duplicate samples from each test medium (without algae)
- Sample storage conditions before analysis: deep-frozen (at about -20 °C) immediately after sampling. - Vehicle:
- no
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Strain: No. 61.81
- Source (laboratory, culture collection): Collection of Algal Cultures (SAG, Institute for Plant Physiology, University of Göttingen, Göttingen, Germany)
- Age of inoculum (at test initiation): not stated
- Method of cultivation: standardized conditions according to the test guidelines
ACCLIMATION
no acclimation - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- 0.24
- Test temperature:
- 23-24 °C
- pH:
- 8.2 at start, 9.1 to 9.6 at end
- Dissolved oxygen:
- not stated
- Salinity:
- not stated
- Conductivity:
- not stated
- Nominal and measured concentrations:
- WAF with nominal loading rate: 0.32, 1.0, 3.2, 10, 32, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 50 ml flasks, filled with 15 ml algal suspension
- Aeration: none
- Type of flow-through (e.g. peristaltic or proportional diluter): none
- Renewal rate of test solution (frequency/flow rate): none
- Initial cells density: 10,000 algal cells per ml
- Control end cells density: 2079000 algal cells (Mean value)
- No. of organisms per vessel: n/a
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): n/a
GROWTH MEDIUM
- Standard medium used: yes
- Detailed composition if non-standard medium was used:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: sterile, purified water
- Total organic carbon: not stated
- Particulate matter: not stated
- Metals: not stated
- Pesticides: not stated
- Chlorine: not stated
- Alkalinity: not stated
- Ca/mg ratio: not stated
- Conductivity: not stated
- Culture medium different from test medium: no
- Intervals of water quality measurement: not stated
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: 24 hours/day
- Light intensity and quality: 8300 Lux
- Salinity (for marine algae): n/a
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: [counting chamber; electronic particle counter; fluorimeter; spectrophotometer; colorimeter]
- Chlorophyll measurement:
- Other:
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Justification for using less concentrations than requested by guideline:
- Range finding study: none - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Loading rate
- Remarks:
- Water accomodated fraction
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Loading rate
- Remarks:
- Water accomodated fraction
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no abnormalities observed (microscopically)
- Unusual cell shape: none
- Colour differences: none
- Flocculation: none
- Adherence to test vessels: none
- Aggregation of algal cells: none - Validity criteria fulfilled:
- yes
- Conclusions:
- In conclusion, Salacos 168 ARV had no inhibitory effect on the growth of Pseudokirchneriella subcapitata up to its solubility limit in test water under the present test conditions.
- Executive summary:
The analytically measured test item concentration in the test medium was below limit of quantification (0.3 mg/L) at the beginning and the end of the test period of 72 hours. Since WAFs of the test item with sparingly soluble components were tested, all reported biological results are based on the loading rates.
The test item had no statistically significant inhibitory effect on the growth (yield and growth rate) of Pseudokirchneriella subcapitata during the test period of 72 hours up to and including the highest nominal test concentration of 100 mg/L (results of Dunnett's tests, one sided, Alpha = 0.05).
The test concentration with the loading rate 100 mg/l was, therefore, determined to be the 72 -hour NOEC. This value might even be higher, but concentrations of the test item above the loading rate of 100 mg/l were not tested in accordance with the EU Commission Directive 92/69/EEC. The 72 Hour LOEC and the 72 hour EC10 and EC50 for the algal growth rate (µ) and yield (y) could not be quanttified due to the absence of a toxic effect of Salacos 168 ARV at the tested concentrations. Accordingly, these parameters were clearly higher than the loading rate of 100 mg/L. The microscopic examination of the algal cells after 72 hours exposure showed no difference getween the algae growing at the test concentration of nominal 100 mg/l and the algal cells in the control. The shape and size of the algal cells growing in test media containing the test item at and up to this concentration were obviously not affected.
In the control the biomass increased by a factor of 208 over 72 hours. The validity criterion of increase of biomass by at least a factor of 16 within three days was fulfilled.The mean coefficient of variation of the daily growth rates was 4.2 % during 72 hours. According to the OECD test guideline, the mean coefficient of variation in the control must not be higher than 35 %. Thus, the validity criterion was fulfilled.
The coefficeint of variation of the average specific growth rates in the replicates of the control after 72 hours was 2.6 %. According to the OECD test guideline, the coefficient of variation must not be higher than 7 %. Thus, the validity criterion was fulfilled.
No remarkable observations were made concerning the appearance of the test media. All test media were clear solutions ghroughout the test period.
At the start of the test, the pH values in the test media and the control were 8.2. At the end of the test, pH values of 9.1 to 9.6 were measured. The water temperature during the test was 23 -24 °C.
Reference
Calculation of coefficient of variations for validity criteria:
biomass at time | Growth rate | |||||
0 h | 24 h | 48 h | 72 h | 0-24 h | 24-48 h | 48-72 h |
1,40 | 9,60 | 66,3 | 302,4 | 1,92529086 | 1,9324268 | 1,51756075 |
1,4 | 8,8 | 63,9 | 286,8 | 1,83827948 | 1,98256764 | 1,50146575 |
1,4 | 9,7 | 69,8 | 284,8 | 1,93565365 | 1,97350812 | 1,40615317 |
1,4 | 9,2 | 68 | 297,2 | 1,88273125 | 2,00030422 | 1,47489761 |
1,4 | 9,1 | 62,6 | 295,8 | 1,87180218 | 1,92849086 | 1,55291827 |
1,4 | 9,7 | 69,1 | 279,3 | 1,93565365 | 1,96342885 | 1,39673174 |
Mean | 1,89823518 | 1,96345442 | 1,47495455 | |||
Standard deviation | 0,0401648 | 0,02831771 | 0,06236812 | |||
Coefficient of variation of average specific growth rate out of the daily/section by section growth rates | ||||||
2,11590204 | 1,44223935 | 4,22847769 | ||||
mean coefficient of variation of the daily/section by section specific growth rate |
||||||
2,59553969 |
Description of key information
One Guideline Study performed. Water accomodated Fractions with loading rates up to 100 mg/l tested.
No toxic effects observed up to the highest loading rate.
Key value for chemical safety assessment:
EC50, EC10 > 100 mg/L, 100 mg/L entered in respective field below
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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