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EC number: 238-942-1 | CAS number: 14871-79-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from authoritative database.
Data source
Reference
- Reference Type:
- other: Authoritative database
- Title:
- bacterial reverse mutation test was performed for the given test chemical
- Author:
- U. S. National Library of Medicine
- Year:
- 2 018
- Bibliographic source:
- HSDB (Hazardous Substances Data Bank); US national Library of Medicine reviewed by SRC, 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: EU Method B13/14
- Principles of method if other than guideline:
- To evaluate the mutagenic potential of test chemical in Salmonella typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102 according to the OECD guideline 471 and the EU Method B13/14.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Sodium phosphinate
- EC Number:
- 231-669-9
- EC Name:
- Sodium phosphinate
- Cas Number:
- 7681-53-0
- Molecular formula:
- H3O2P.Na
- IUPAC Name:
- Sodium hypophosphite
- Test material form:
- solid
- Details on test material:
- - Name of test material: sodium phosphinate
- IUPAC name: Sodium hypophosphite
- Molecular formula: NaO2P
- Molecular weight: 87.9778 g/mole
- Smiles notation: [Na+].[O-]P=O
- InChl: 1S/Na.H3O2P/c;1-3-2/h;3H2,(H,1,2)/q+1;/p-1
- Substance type: Inorganic
- Physical state: Solid
Constituent 1
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- the S9 mix, prepared from a liver post-mitochondrial fraction (S9 fraction) of rats induced with Aroclor 1254.
- Test concentrations with justification for top dose:
- - First mutagenicity experiment with and without S9 mix: 0, 312.5, 625, 1250, 2500 and 5000 µg/plate
- Second mutagenicity experiment with and without S9 mix: 0, 625, 1250, 2500, 3750 and 5000 µg/plate - Vehicle / solvent:
- vehicle was used.
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Remarks:
- not specified
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- NUMBER OF REPLICATIONS:
- Number of cultures per concentration - triplicate
- Number of independent experiments - two independent experiments
METHOD OF TREATMENT/ EXPOSURE:
- Cell density at seeding (if applicable): No data
- Test substance added -1st experiment (+S9/-S9) and 2nd experiment (-S9): in agar (plate incorporation); 2nd experiment (+S9): preincubation method
TREATMENT AND HARVEST SCHEDULE:
- Preincubation period, if applicable: 60 minutes (only in second mutagenicity test with S9 mix)
- Exposure duration/duration of treatment: 48 to 72 hours - Rationale for test conditions:
- Not specified
- Evaluation criteria:
- The evaluation of the toxicity was performed based on the observation of the decrease in the number of revertant colonies and/or a thinning of the bacterial lawn.
- Statistics:
- Not Specified
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- True negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation and time of the determination: No precipitate was observed in the petri plates when scoring the revertants at all dose-levels.
RANGE-FINDING/SCREENING STUDIES (if applicable): A preliminary toxicity test was performed to define the dose-levels of test chemical to be used for the mutagenicity study.
Ames test:
- Signs of toxicity - No toxicity was noted towards all the strains used, both with and without S9 mix. - Remarks on result:
- other: No mutagenic effects were observed
Applicant's summary and conclusion
- Conclusions:
- The test chemical was evaluated for its mutagenic potential in Salmonella typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102 according to the OECD guideline 471 and the EU Method B13/14. Under these experimental conditions the test chemical did not show any mutagenic activity in the bacterial reverse mutation assay, thus, the test chemical was considered as non-mutagenic.
- Executive summary:
In Genetic toxicity in vitro study, the given test chemical was evaluated for its mutagenic potential in Salmonella typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102 according to the OECD guideline 471 and the EU Method B13/14. A preliminary toxicity test was performed to define the dose-levels of test chemical to be used for the mutagenicity study. The test chemical was then tested in two independent experiments, both with and without a metabolic activation system, the S9 mix, prepared from a liver post-mitochondrial fraction (S9 fraction) of rats induced with Aroclor 1254. Both experiments were performed according to the direct plate incorporation method except for the second test with S9 mix, which was performed according to the preincubation method (60 minutes, 37 deg C). The five strains of bacteria Salmonella typhimurium: TA 1535, TA 1537, TA 98, TA 100 and TA 102 were exposed to the following dose levels of test chemical (three plates/dose-level): 312.5, 625, 1250, 2500 and 5000 ug/plate, for the first mutagenicity experiment with and without S9 mix; 625, 1250, 2500, 3750 and 5000 ug/mL for the second mutagenicity experiment with and without S9 mix. After 48 to 72 hours of incubation at 37 deg C, the revertant colonies were scored. The evaluation of the toxicity was performed based on the observation of the decrease in the number of revertant colonies and/or a thinning of the bacterial lawn. The number of revertants for the vehicle and positive controls was as specified in the acceptance criteria. The study was therefore considered valid. No precipitate was observed in the petri plates when scoring the revertants at all dose-levels. No toxicity was noted towards all the strains used, both with and without S9 mix. The test chemical did not induce any significant increase in the number of revertants, both with or without S9 mix, in any of the five strains. Under these experimental conditions, the given test chemical did not show any mutagenic activity in the bacterial reverse mutation assay, thus, it was considered as non-mutagenic.
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