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EC number: 408-090-7 | CAS number: 100418-33-5 METHYLGELB
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-((4-methyl-2-nitrophenyl)amino)ethanol
- EC Number:
- 408-090-7
- EC Name:
- 2-((4-methyl-2-nitrophenyl)amino)ethanol
- Cas Number:
- 100418-33-5
- Molecular formula:
- C9H12N2O3
- IUPAC Name:
- 2-[(4-methyl-2-nitrophenyl)amino]ethan-1-ol
- Test material form:
- solid: particulate/powder
- Details on test material:
- orange-red
Constituent 1
- Specific details on test material used for the study:
- Chemical Name: 1-(2'-Hydroxyethyl)-amino-4-methyl-2-nitro-benzene
Batch Data: B 3/89
Purity: 98%
Molecular Mass: 196 g/mole
Formula: C9H12N2O3 - Radiolabelling:
- no
Study design
- Details on sampling:
- The test article was dissolved in the buffer solutions specified above. For this purpose a stock solution containing 10 mg of the test article per 100 ml water was diluted with buffer solution to yield a test article concentration of 10 mg/l.
- Buffers:
- Buffer solutions were prepared as follows, according to OECD guideline 111:
Citrate buffer pH 4.0 according to Kalthoff & Vleeschhouwer:
100 ml of 0.1-M-potassium dihydrogencitrate solution and 18 ml of 0.1-N-NaOH were diluted to 200 ml with water. The pH of this solution was adjusted to 4.0 with additional 0.1-N-NaOH.
Phosphate buffer pH 7.0 according to Clark & Lubs:
100 ml of 0.1-M-potassium dihydrogenphosphate solution and 59.3 ml of 0.1-N-sodium hydroxide solution were diluted to 200 ml with water. The pH of this solutionwas adjusted to 7.0 with additional 0.1-N-NaOH.
Borate buffer pH 9.0 according to Clark & Lubs:
3 g of boric acid and 3. 7 g of potassium chloride were dissolved in 500 ml of water to yield a concentration of 0.1 mol/l for each compound. 100 ml of this solutionplus 42.6 ml of 0.1-N-sodium hydroxide solution were diluted with water ad 200 ml. The pH of this solution was adjusted to 9.0 with additional 0.1-N-NaOH.
In all cases, water was degassed by bubbling nitrogen through the liquid for about 20 min immediately before use.
Results and discussion
- Preliminary study:
- Initially, an orientating preliminary test at 50 °C and pH values of 4.0, 7.0 and 9.0 was performed as specified by OECD guideline 111.
- Test performance:
- The test article was found to exhibit no degradation in excess of 10 % during the preliminary test. Therefore, in accordance witb OECD guideline 111, no main testwas performed.
- Transformation products:
- no
Total recovery of test substance (in %)open allclose all
- % Recovery:
- >= 100 - <= 100
- pH:
- 4
- Temp.:
- 50 °C
- Duration:
- >= 1 - <= 5 d
- Remarks on result:
- hydrolytically stable based on preliminary test
- % Recovery:
- >= 100 - <= 100
- pH:
- 7
- Temp.:
- 50 °C
- Duration:
- >= 1 - <= 5 d
- Remarks on result:
- hydrolytically stable based on preliminary test
- % Recovery:
- >= 99 - <= 100
- pH:
- 9
- Temp.:
- 50 °C
- Duration:
- >= 1 - <= 5 d
- Remarks on result:
- hydrolytically stable based on preliminary test
Dissipation DT50 of parent compoundopen allclose all
- Key result
- pH:
- 4
- Temp.:
- 50 °C
- Remarks on result:
- hydrolytically stable based on preliminary test
- Key result
- pH:
- 7
- Temp.:
- 50 °C
- Remarks on result:
- hydrolytically stable based on preliminary test
- Key result
- pH:
- 9
- Temp.:
- 50 °C
- Remarks on result:
- hydrolytically stable based on preliminary test
- Details on results:
- The test article stock solution was diluted with the individual buffers (pH 4, 7 and 9) as indicated above and the solutions were filtered through 0.22 µL disposable filter units. Duplicate samples of each buffered solution were prepared and transferred to 50 ml glass flasks. A sample each was taken to determine the initial concentration of the test article. The flasks were sealed with aluminium crimp caps and silicone ruhher septa and incubated in a water bath at 50 °C. Care was taken to include only small volumes of air in these vessels. Samples were taken after 1, 3 and 4 days and analysed by HPLC as described. Since in this period no degradation was observed, the test was finished after this interval. For a duplicate performance of this test, sample concentrations were determined initially and after 4 days.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test article was subjected to a preliminary test for hydrolytic stability, applying a temperature of 50 °C at pH 4.0, 7.0 and 9.0. Under these conditions, no continuous decrease in concentration was observed. Methylgelb can therefore be considered hydrolytically stable according to OECD guideline 111.
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