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EC number: 224-580-1 | CAS number: 4418-26-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- Strain with AT base pair missing; not tested up to the maximum test concentration for soluble non-cytotoxic substances
Data source
Reference
- Reference Type:
- publication
- Title:
- Salmonella Mutagenicity Tests: III. Results From the Tesing of 255 Chemicals
- Author:
- Zeiger et al.
- Year:
- 1 987
- Bibliographic source:
- Environ Mutagen. 9:1-109
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- adopted 21 July 1997
- Deviations:
- yes
- Remarks:
- Strain with AT base pair missing; not tested up to maximum test concentration for soluble non-cytotoxic substances
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Sodium 1-(3,4-dihydro-6-methyl-2,4-dioxo-2H-pyran-3-ylidene)ethanolate
- EC Number:
- 224-580-1
- EC Name:
- Sodium 1-(3,4-dihydro-6-methyl-2,4-dioxo-2H-pyran-3-ylidene)ethanolate
- Cas Number:
- 4418-26-2
- Molecular formula:
- C8H7O4.Na
- IUPAC Name:
- sodium 1-(6-methyl-2,4-dioxo-2H-pyran-3(4H)-ylidene)ethanolate
Constituent 1
Method
- Target gene:
- his operon
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- cofactor supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of rats and hamsters treated with Aroclor 1254.
- Test concentrations with justification for top dose:
- 33, 100, 333, 1000 and 1820 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: water
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive control substance:
- other: -S9: sodium azide for TA100 and TA1535; 9-aminoacridine for TA1537; 4-nitro-o-phenylendiamine for TA98; +S9: 2 aminoanthracene (2.5 or 5 µg/plate) for all strains
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 20 min
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: Triplicates each in 2 independent experiments
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth - Evaluation criteria:
- A chemical was judged to be mutagenic or weakly mutagenic if it produced a reproducible, dose-related increase in his+ revertants over the corresponding solvent controls in replicate trials. A chemical was considered to be questionable if a reproducible increase of his+ revertants did not meet the criteria for either mutagenic or weakly mutagenic, or if only single doses produced an increase in his+ revertants in repeat trials.
- Statistics:
- Mean values and standard deviation were calculated.
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- other: TA 100, TAA 1535 and TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- -S9: ≥ 1000 µg/plate; + S9: ≥1820 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES: The test substance was tested inititally in a toxicity assay to determine the appropriate dose range (data not shown).
Any other information on results incl. tables
Table 1 Test Results
With or without S9-Mix | Test substance concentration | Mean number of revertant colonies per plate | |||
(μg/plate) | (average of 3 plates ± Standard deviation) | ||||
Base-pair substitution type | Frameshift type | ||||
TA 100 | TA1535 | TA98 | TA1537 | ||
– | 0 | 122 ± 24.2 | 5 ± 1.7 | 14 ± 2.5 | 4 ± 2.0 |
– | 33 | 107 ± 2.9 | 6 ± 0.9 | 12 ± 0.7 | 5 ± 1.2 |
– | 100 | 115 ± 14.1 | 6 ± 1.3 | 11 ± 0.3 | 5 ± 2.2 |
– | 333 | 104 ± 5.5 | 3 ± 0.9 | 12 ± 0.6 | 5 ± 0.6 |
– | 1000 | 100 ± 5.8 | 4 ± 1.2 | 8 ± 1.9 | 3 ± 0.9 |
– | 1820 | 85 ± 9.5 | 4 ± 1.0 | 8 ± 0.9 | 4 ± 1.8 |
Positive controls, –S9 | Name | SA | SA | 4-NOP | 9-AA |
Mean No. of colonies/plate (average of 3 ± SD) | 571 ± 9.9 | 168 ± 5.9 | 407 ± 11.3 | 1088 ± 11.7 | |
+ | 0 | 158 ± 5.0 | 6 ± 2.3 | 26 ± 4.5 | 6 ± 1.8 |
+ | 33 | 148 ± 12.2 | 4 ± 1.2 | 17 ± 4.2 | 6 ± 1.2 |
+ | 100 | 161 ± 14.7 | 5 ± 0.6 | 26 ± 1.0 | 5 ± 1.2 |
+ | 333 | 146 ± 12.7 | 7 ± 2.0 | 17 ± 1.5 | 4 ± 1.2 |
+ | 1000 | 134 ± 28.7 | 5 ± 0.3 | 17 ± 2.6 | 7 ± 1.7 |
+ | 1820 | 138 ± 23.6 | 6 ± 1.3 | 11 ± 2.0 | 5 ± 1.7 |
Positive controls, +S9 (rat) | Name | 2AA | 2AA | 2AA | 2AA |
Mean No. of colonies/plate (average of 3 ± SD) | 3149 ± 19.0 | 80 ± 4.7 | 1621 ± 71.4 | 129 ± 9.2 |
Applicant's summary and conclusion
- Conclusions:
- Dehydroacetic acid, sodium salt DHA-Na (with or without metabolic activation) was not mutagenic in this Ames test.
- Executive summary:
Dehydroacetic acid, sodium salt DHA-Na assessed in an Ames test at concentrations up to 1820µg/plate (with or without metabolic activation ) was shown to be not mutagenic.
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