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EC number: 229-880-6 | CAS number: 6805-41-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1994
- Reliability:
- 1 (reliable without restriction)
- Justification for type of information:
- option an in vitro skin irritation study does not need to be conducted because adequate data from an in vivo skin irritation study are available"."
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD short-term and long-term Toxicology group. Final report: Acute dermal irritation & corrosion (1981)
- Version / remarks:
- 1981
- Deviations:
- no
- Principles of method if other than guideline:
- Skin preparation
Approximately 24 hours before the test fur was removed from an area approximateky 240 cm2 wide by clipping and shaving the dorsal and flank zones of the animals. Two areas of the back, spaced approximately 10 cm aport, was designed for the position of the patches. On area was abraded by making four minor epidermal incision along parallel lines approximately 3 cm long and 0.5 cm apart. The incision were through the statum corneum only and were not sufficiently deep as to produce bleeding.
Administration
Square gauze pads 2.5x2.5 cm fixed to adhesive tape were used 0.5 ml/animal of the assay sample were placed under the pad, which was kept in place by means of thin bands of adhesive hypoallergenic tape. The entire trunk of the animal was then wrapped with rubberized cloth.
Removal of the patches
The patches were removed 24 hours after the application. The exceeding paste was then washed away from skin using a pad soaked in distilled water
Experimental design
Each animal served as its own control, having a treated and a non-treated area, the first on the right side and the latter on the left side of its back. - GLP compliance:
- not specified
Test material
- Reference substance name:
- Escin
- EC Number:
- 229-880-6
- EC Name:
- Escin
- Cas Number:
- 6805-41-0
- Molecular formula:
- C110H172O48
- IUPAC Name:
- (2S,3S,4S,5R,6R)-6-{[(3S,4S,4aR,6aR,6bS,8R,8aR,9R,10R,12aS,14aR,14bR)-9-(acetyloxy)-8-hydroxy-4,8a-bis(hydroxymethyl)-4,6a,6b,11,11,14b-hexamethyl-10-[(2-methylbutanoyl)oxy]-1,2,3,4,4a,5,6,6a,6b,7,8,8a,9,10,11,12,12a,14,14a,14b-icosahydropicen-3-yl]oxy}-4-hydroxy-3,5-bis({[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy})oxane-2-carboxylic acid; (2S,3S,4S,5R,6R)-6-{[(3S,4S,4aR,6aR,6bS,8R,8aR,9R,10R,12aS,14aR,14bR)-9-(acetyloxy)-8-hydroxy-4,8a-bis(hydroxymethyl)-4,6a,6b,11,11,14b-hexamethyl-10-{[(2E)-2-methylbut-2-enoyl]oxy}-1,2,3,4,4a,5,6,6a,6b,7,8,8a,9,10,11,12,12a,14,14a,14b-icosahydropicen-3-yl]oxy}-4-hydroxy-3,5-bis({[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy})oxane-2-carboxylic acid
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- Escin free acid Batch 6033
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
Test system
- Preparation of test site:
- other: clipping and shaving
- Vehicle:
- water
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- 0.5 ml/animal
- Duration of treatment / exposure:
- 24 hours
- Observation period:
- 72 hours
- Number of animals:
- 6
- Details on study design:
- Skin preparation
Approximately 24 hours before the test fur was removed from an area approximateky 240 cm2 wide by clipping and shaving the dorsal and flank zones of the animals. Two areas of the back, spaced approximately 10 cm aport, was designed for the position of the patches. On area was abraded by making four minor epidermal incision along parallel lines approximately 3 cm long and 0.5 cm apart. The incision were through the statum corneum only and were not sufficiently deep as to produce bleeding.
Administration
Square gauze pads 2.5x2.5 cm fixed to adhesive tape were used 0.5 ml/animal of the assay sample were placed under the pad, which was kept in place by means of thin bands of adhesive hypoallergenic tape. The entire trunk of the animal was then wrapped with rubberized cloth.
Removal of the patches
The patches were removed 24 hours after the application. The exceeding paste was then washed away from skin using a pad soaked in distilled water
Experimental design
Each animal served as its own control, having a treated and a non-treated area, the first on the right side and the latter on the left side of its back.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- edema score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- edema score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- edema score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Remarks on result:
- no indication of irritation
Applicant's summary and conclusion
- Interpretation of results:
- other: not irritant
- Remarks:
- acording to Draze method
- Conclusions:
- After 24 hours in 2 and 6 treated animals a slight erythema was observed. After 72 hours this erythema was disappared. results referring to the single animals and mean values of erythema and oedema reffering to each observation time are shown in Attachement.
The score of primary skin was: 0165.
According to Classification Draize method: Less than 0.5: NOT IRRITANT
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