Ethyl undec-10-enoate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 - 28 Jul 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

yes

Remarks:

version 92/69/EWG of 1992

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Isopropyl myristate

EC Number:

203-751-4

EC Name:

Isopropyl myristate

Cas Number:

110-27-0

Molecular formula:

C17H34O2

IUPAC Name:

isopropyl myristate

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method 1: direct addition of 100 mg/L, 5 min ultrasonic treatment
- Method 2: direct addition of 100 mg TS into 1 L M4 medium; 24 h treatment with magnetic stirrer, undissolved test substance removed via filtration through glass fibre filter (pretreated with NaOH)
- Method 3: 3-5 times saturation: 0.5 mg product into 3 L of M4 medium, 24 h treatment with magnetic stirrer

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: in-house culture, originally obtained from BGA Berlin, May 1994
- Feeding during test: none


ACCLIMATION
- Type and amount of food: Scenedesmus subspicatus

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Test temperature:

21.9 - 22.0 °C

pH:

8.0 - 8.1

Dissolved oxygen:

>= 91% (saturation)

Nominal and measured concentrations:

nominal: 100 mg/L
Method 1 measured: 39.96 mg/L at 0 hrs, 0.41 mg/L at 48 h
Method 2 measured: 0.23 mg/L at 0 hrs, <0.01 mg/L at 48 h
Method 3 measured: <0.01 mg/L at 0 hrs, <0.01 mg/L at 48 h

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 mL beaker
- Type: closed by clockglasses
- Material, size, headspace, fill volume: 50 mL testing solution
- No. of vessels per concentration (replicates): 4 vessels with 5 organims each
- No. of vessels per control (replicates): 2 vessels with 10 organisms each

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light/ 8 hours dark

EFFECT PARAMETERS MEASURED: immobility after 24 and 48 h

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

< 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: method 1

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.05 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

other: filtered test solution

Basis for effect:

mobility

Remarks on result:

other: method 2

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.05 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

other: 3-5 times saturation concentration

Basis for effect:

mobility

Remarks on result:

other: method 3

Details on results:

- Solubility limit was found <0.05 mg/L

Any other information on results incl. tables

Table 1: Immobility in % with method 1 (direct addition of 100 mg in 1 L test solution, 5 minutes ultrasonic treatment)

Concentration	Incubation time (in h)
in mg/L	0	48
0 (1)	0	10*
0 (2)	0	0
100 (1)	20	100**
100 (2)	0	0 */**
100 (3)	0	80 */**
100 (4)	0	80 */**

* animals swimming at water surface

** oil film at surface

Test solutions with direct substance addition were slightly turbid.

Table 2: Immobility in % with method 2 (24 hours magnetic stirring, removal of undissolved TS via filtration)

Concentration	Incubation time (in h)
in mg/L	0	48
0 (1)	0	10*
0 (2)	0*	10*
100 (1)	0	0
100 (2)	0	20
100 (3)	0	0
100 (4)	0	0

Table 3: Immobility in % with method 3 (0.5 mg product into 3 L medium, 24 hours with magnetic stirrer)

Concentration	Incubation time (in h)
in mg/L	0	48
0 (1)	0*	10*
0 (2)	0	0
0.15-0.25 (1)	0	0
0.15-0.25 (2)	0	0
0.15-0.25 (3)	0*	0*
0.15-0.25 (4)	0*	0*