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EC number: 203-783-9 | CAS number: 110-61-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Deviations:
- yes
- Remarks:
- Animals where housed with 2 in a cage during acclimatisation. The animals showed normal during the acclimatization period, it was considered not to affect the animals and study results.
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- acute toxic class method
Test material
- Reference substance name:
- Succinonitrile
- EC Number:
- 203-783-9
- EC Name:
- Succinonitrile
- Cas Number:
- 110-61-2
- Molecular formula:
- C4H4N2
- IUPAC Name:
- butanedinitrile
- Details on test material:
- - Analytical purity: 97%
- Source: Aldrich Chemical Company, Milwaukee, WI, USA
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: DSM Engineering Plastics B.V. lotnumber SN0620140520
- Expiration date of the lot/batch: 2016.07.01
- Purity test date: 99.93%
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Store in a cool area (IO-20°C). Containers that have been opened must be filled with dry nitrogen for at least 1min and then sealed. Be careful not to import any water or impurities during the filling and sealing course.
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: Easily soluble in cold water
FORM AS APPLIED IN THE TEST white waxy solid
Test animals
- Species:
- rat
- Strain:
- other: Sprague Dawley (SD)
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Supplier: Beijing Vital River Laboratory Animal Technology Co., Ltd.
License No.: SCXK (Jing) 2012—0001, Qualification: 11400700112907.
- Age at study initiation: 51-60 days on arrival, in the range of 8-12 weeks at the commencement of each
animal’s dosing.
- Weight at study initiation: 182-222g
- Housing:Animals were raised
in suspended, stainless steel cages (L32.0cm >(L167.0cmXW70.0cm> Animals were housed individually during the test.
- Diet : diet with complete nutrition supplied by Beijing keaoxieli Feed Co., Ltd. Product License No: SCXKUing) 2012-0001, Batch NO. 15063223).
- Water (e.g. ad libitum): purified water (by HT-R01000 purity system.)
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22.5-24.5 °C
- Humidity (%): 45%-70%
- Air changes (per hr):
- Photoperiod (hrs dark / hrs light): lighting sequence was 12 hours light, 12 hours dark.
Administration / exposure
- Route of administration:
- oral: gavage
- Doses:
- 300 mg/kg BW and 2000 mg/kg BW
- No. of animals per sex per dose:
- 3
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: 30 min and at l, 2 and 4 hours after dosing and then once each day for up to 14 days.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight,organ weights, histopathology, other:changes in skin and fur, eyes and mucous membranes, and also respiratory, circulatory, autonomic and central nervous systems, and somatomotor activity and behaviour pattern. Attention had been directed to observations of tremors, convulsions, salivation, diarrhoea, lethargy, sleep and coma.
Results and discussion
- Preliminary study:
- LD50 is 488.7 mg/kg b.w. for rats was available. Based on that value the test doses were selected.
Effect levels
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- >= 300 - <= 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- Mortality
Dose Level-The first dosing (300mg/kg): One animal was dead on Day 2 after dosing. Dose Level-The second dosing (300mg/kg): There were no deaths or moribund during the test. Dose Level-The third dosing (2000mg/kg): All animals were dead in 4h after dosing. Mortality summary results were given in appendix Table 1. - Clinical signs:
- other: Dose Level-The first dosing (300mg/kg): Animals showed decrease in locomotor activity, emaciation, and soiled perinea region after dosing. Dose Level-The second dosing (3 00mg/kg): There were no abnormal findings after dosing from the first day until the end
- Gross pathology:
- Necropsy
No abnormalities were found at necropsy.
Necropsy results see appendix table 4.
Any other information on results incl. tables
Table 1. Mortality summary results | |||||||||||||||||||
Dosing sequence |
Dose (mg/kg) |
sex | Animal amounth |
Time (d) | Mortality | ||||||||||||||
0 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | |||||
1 | 300 | Female | 3 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1/3 |
2 | 300 | Female | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0/3 |
3 | 2000 | Female | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3/3 |
Table 2. Individual Clinical observations | |||||||||||||||||||
Dose (mg/kg) |
Animal ID | Symptons After Dosing (Hours) |
Symptoms During Period After Dosing (Days) |
||||||||||||||||
0.5 | 1 | 2 | 4 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | ||
300 | 2100 | N | N | N | N | S | E1D | - | - | - | - | - | - | - | - | - | - | - | - |
2101 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
2102 | N | N | N | N | H | N | N | N | N | N | N | N | N | N | N | N | N | N | |
300 | 2200 | N | N | N | N | N | E1D | - | - | - | - | - | - | - | - | - | - | - | - |
2201 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
2202 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
300 | 2300 | N | SE | SE Se | FD | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
2301 | N | SE | SE SeW | FD | - | - | - | - | - | - | - | - | - | - | - | - | - | - | |
2302 | N | N | SE | FD | - | - | - | - | - | - | - | - | - | - | - | - | - | - | |
Note: N=no sign; S=decrease in locomotor activity; E1=emaciation, E=secretion around mouth and nouse, Se-semi closed eyes, W=weak breath, D=death, F=soiled fur around the mouth and nouse, H=soiled perinea region. |
Table 3. Individual and Mean Body Weights | |||||||||
Dose (mg/kg) |
Dose (mg/kg) |
Animal ID | Body Weight (g) at Day | Body Weight Gain (g) During Week | |||||
Grouping | Day0 | Day7 | Day14 | At Death | 1 | 2 | |||
300 | Female | 2100 | 204 | 191 | - | - | 149 | - | - |
2101 | 233 | 212 | 246 | 268 | - | 34 | 22 | ||
2102 | 226 | 209 | 255 | 263 | - | 46 | 8 | ||
Mean +/- SD | 221 +/- 15 | 204 +/- 11 | 251 +/- 6 | 266 +/- 4 | - | 40 +/- 8 | 15 +/- 10 | ||
N | 3 | 3 | 2 | 2 | 1 | 2 | 2 | ||
300 | Female | 2200 | 201 | 183 | - | - | 149 | - | - |
2201 | 219 | 212 | 246 | 268 | - | 34 | 22 | ||
2202 | 225 | 209 | 255 | 263 | - | 46 | 8 | ||
Mean +/- SD | 215+/- 12 | 199 +/- 15 | 245 +/- 16 | 226 +/- 11 | - | 46 +/- 9 | -20+/-6 | ||
N | 3 | 3 | 3 | 3 | - | 3 | 3 | ||
2000 | Female | 2300 | 196 | 191 | - | - | 184.53 | - | - |
2301 | 216 | 212 | - | - | 199.86 | - | - | ||
2302 | 222 | 209 | - | - | 203.18 | - | - | ||
Mean +/- SD | 211 +/- 14 | 198 +/- 7 | - | - | - | - | - | ||
N | 3 | 3 | 0 | 0 | 3 | 2 | 2 | ||
Note: body weight(g), N=Number of animals per group, SD= Standard deviation |
Table 4. Indivudual Negroscopy Findings | ||||
Animal ID | sex | Dose (mg/kg) |
Death time | Findings |
2100 | Female | 300 | Day2 | No abnormalities |
2101 | Female | 300 | Sheduled Negroscopy | No abnormalities |
2102 | Female | 300 | Sheduled Negroscopy | No abnormalities |
2200 | Female | 300 | Sheduled Negroscopy | No abnormalities |
2201 | Female | 300 | Sheduled Negroscopy | No abnormalities |
2202 | Female | 300 | Sheduled Negroscopy | No abnormalities |
2300 | Female | 2000 | Day 0 | No abnormalities |
2301 | Female | 2000 | Day 0 | No abnormalities |
2302 | Female | 2000 | Day 0 | No abnormalities |
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- Based on the results, the acute oral LDso in the rats for Succinonitrile was estimated to be among 300 mg/kg b.w. - 2000 mg/kg b.w..
According to the GHS’s classification criteria for acute oral toxicity, the test item was classified as Category 4. - Executive summary:
Introduction: The study was performed to assess the acute oral toxicity of Succinonitrile in Sprague Dawley rats. The method was designed to meet the OECD Guideline for Testing of Chemicals: Acute Oral Toxicity-Acute Toxic Class Method (TG423, 2001).
Method: The test item was tested using a stepwise procedure, each group using three female animals. The first step dosing and second step dosing were 300 mg/kg and the third step dosing was ZOOOmg/kg. Clinical observations and bodyweight were monitored during the study. All animals were subjected to a gross necropsy.
Results:
Mortality
Dose Level-The first dosing (300mg/kg): One animal was dead on Day 2 after dosing.
Dose Level-The second dosing (300mg/kg): There were no deaths or moribund during
the test.
Dose Level-The third dosing (2000mg/kg): All animals were dead in 4h after dosing.
Clinical observations
Dose Level-The first dosing (300mg/kg): Animals showed decrease in locomotor
activity, emaciation, and soiled perinea region after dosing.
Dose Level-The second dosing (3 00mg/kg): There were no abnormal findings after
dosing from the first day until the end of the test.
Dose Level-The third dosing (2000mg/kg): Animals showed decrease in locomotor
activity, emaciation, secretion around mouth and nose, semi-closed eyes, weak breath,
soiled fur around the mouth and nose after dosing.
Body Weight
All surviving animals showed a increase in body weight during the first week, but most of the surviving animals showed a decrease during the second week.
Necropsy
No abnormalities were found at necropsy.
Conclusion:
Based on the results, the acute oral LDso in the rats for Succinonitrile was estimated to be among 300 mg/kg b.w. - 2000 mg/kg b.w.. According to the GHS’s classification criteria for acute oral toxicity, the test item was classified as, Category 4.
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