lithium bis(fluorosulfonyl)imide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

The study was conducted between 16 September 2010 and 13 December 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Biodegradation Test of Chemical Substances prescribed in "Test Methods for New Chemical Substances", Pharmaceutical and Food Safety Bureau No.1121002 of MHLW, Manufacturing Industries Bureau No. 2 of METI and Environment Policy Bureau No.031121002 of MOE.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

lithium(1+) bis(fluorosulfonyl)azanide

EC Number:

924-516-9

Cas Number:

171611-11-3

Molecular formula:

(FSO2)2NLi

IUPAC Name:

lithium(1+) bis(fluorosulfonyl)azanide

Specific details on test material used for the study:

Name: Lithium Bis(fluorosulfonyl) lmide
Lot No.: 100527475
Purity: 99.5% (Sponsor's information)
Received date: July 6, 2010
Expiry date: May 27, 2011
Storage condition: The substance in a glass bottle was covered with the aluminum lamination bag, and was stored at room temperature (dark).
The test substance was weighed as 100% of purity.

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

Standard Activated Sludge
Supplier: Chemicals Evaluation and Research Institute, Japan
Received date: October 14, 2010
Lot No.: 201009R
Suspended solid concentration (MLSS): 3100 mg/L (October 18, 2010)

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

Experimental Methods
Biological oxygen demand (BOD) which occurred by the degradation of the test substance in the presence of the standard activated sludge was measured periodically (28 days) with the enclosed respirometer. After the last measurement of BOD, the concentration of the test substance was determined with LC/MS/MS and AA. And then the biodegradability of the test substance was evaluated from the results of the BOD and the concentration of the test substance.

Conditions
Temperature: 24.7 - 24. 9°C (actual measurement)
Exposure period: 28 days (BOD measurement)
Volume: 300 ml
Concentrations:
Test substance and aniline (standard substance): 100 mg/L
Standard activated sludge (MLSS): 30 mg/L
Experimental Design and Additi on of the Test Substance

Bottle No. 1 : [Water + Test substance] system
Deionized water (300 ml) was transferred into a bottle and then the test substance (30 mg) was added to the water.

Bottle No. 2-No. 4: [Sludge + Test substance] system
Mineral medium* (297.1 ml) was transferred into 3 bottles each and then 30 mg of the test substance was added into each bottle, followed by the addition of the standard activated sludge** (2.9 ml) to each bottle.

Bottle No. 5: [Sludge + Aniline] system
Mineral medium* (297.1 ml) was transferred into a bottle and then 29 μL (30 mg) of aniline was added into the bottle with a micropipette. After mixing it well, the standard activated sludge** (2.9 ml) was added to the bottle.

Bottle No. 6: [Sludge blank] system
Mineral medium* (297.1 ml) was transferred into a bottle and the standard activated sludge** (2.9ml) was added to the medium.

*) A, B, C and D solutions (each 6 ml) were added to deionized water to make a 2-L solution.

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

% Degradation 1

Parameter:

other: % degradation based on BOD

Value:

2

Sampling time:

28 d

% Degradation 2

Parameter:

% degradation (test mat. analysis)

Remarks:

LC/MS/MS

Value:

0

Sampling time:

28 d

% Degradation 3

Parameter:

other: % degradation based on AA measurement of lithium

Value:

-4

Sampling time:

28 d

Details on results:

Observation Results of the Contents
The test solutions of the [Sludge + Test substance] system ( Bottle Nos. 2 - 4) and the [Sludge blank] system (Bottle No. 6) were slightly muddy white. The test solution of the [Sludge + Aniline] system (Bottle No. 5) was muddy white and that of the [Water + Test substance] system (Bottle No. 1) was colorless and transparent. The multiplication of activated sludge was observed in the [sludge + aniline] system only.

Degradability Based on the BOD Measurement
The BOD values after 28 days for the [Sludge + Test substance] system were 1. 9380 mg for bottle No. 2, 2.0440 mg for bottle No. 3 and 1.9960 mg for bottle No. 4. The BOD values
for the [Sludge blank] and [Water + Test substance] systems were 1.7450 mg and 0.0000 mg, respectively. Since the TOD of the test substance was 10.3 mg, the BOD degradabilities of the [Sludge + Test substance] system were 2% for bottle No. 2, 3% for bottle No.3 and 2% for bottle No. 4. Therefore, the mean degradability was calculated to be 2% . The difference of the degradation rates between maximum and minimum was less than 20% so the validity criterion of the biodegradation test was fulfilled.

Degradability Based on the LC/MS/MS Measurement
The amounts of the residual test substance in the [Sludge + Test substance] system were 31.1 mg for bottle No. 2, 29.5 mg for bottle No. 3 and 30.9 mg for bottle No. 4. The amount
of the residual test substance in the [Water + Test substance] system was 30.4 mg. The degradation rates of the [Sludge + Test substance] system were -2% for bottle No. 2, 3% for bottle No. 3 and -2% for bottle No. 4. Therefore, the mean degradability was calculated as 0%. The difference of the degradation rates between maximum and minimum was less than 20% so the validity criterion of the biodegradation test was fulfilled. No unknown peak which had larger area than the LOQ was observed on the LC/MS/MS chromatogram of the [Sludge + Test substance] system.

Degradability Based on the AA Measurement
The amounts of the residual lithium in the [Sludge + Test substance] system were 1.13 mg for bottle No. 2, 1.09 mg for bottle No.3 and 1.12 mg for bottle No. 4. The amount of the residual lithium in the [Water + Test substance] system was 1.07 m g . The degradation rates of the [Sludge + Test substance] system were -6% for bottle No. 2, -2% for bottle No. 3 and - 5% for bottle No. 4. Therefore, the mean degradability was calculated as -4%. The difference of the degradation rates between maximum and minimum was less than 20% so the validity criterion of the biodegradation test was fulfilled.

BOD5 / COD results

Results with reference substance:

Confirmation of the Degradation Activity of the Activated Sludge
The BOD degradabilities of aniline were 51% (7 days after the exposure) and 65% (14 days after the exposure). Therefore, it was considered that the activated sludge had good activity. Thus, the validity of this study was confirmed.

Any other information on results incl. tables

Results of the pH Measurement

At the start of the cultivation, the pH values of the [Water + Test substance] system, the [Sludge + Test substance] system, the [Sludge + Aniline] system and the [Sludge blank] system solutions were 5.3, 7.3, 7.3 and 7.3, respectively. At the end of the cultivation, the pH values of each system solution were 6.4, 7.3 (mean value), 8.4 and 7.7, respectively.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

The biodegradation test of Lithium Bis(fluorosulfonyl) lmide (28 days) was conducted. The mean BOD degradability was 2% (n=3). The mean degradabilities calculated from the results of the LC/MS/MS and AA measurements were 0% (n=3) and -4% (n=3), respectively. Therefore, it was considered that Lithium Bis(fluorosulfonyl) lmide is not ready biodegradable because the degradabilities for all vessels were less than 60% (Test Methods and Judgment Criteria for the Designation of Monitoring Chemical Substances, April 12, 2010).

Executive summary:

The biodegradation test (28 days) of Lithium Bis(fluorosulfonyl) lmide was conducted and the mean degradability calculated from the biological oxygen demand (BOD) was 2% (n=3). Furthermore, the mean degradabilities calculated from the residual amount of the test substance obtained by measurement with the liquid chromatograph mass spectrometer and the atomic absorption spectrophotometer were 0% (n=3) and -4% (n=3), respectively. It was considered that Lithium Bis(fluorosulfonyl) lmide is not ready biodegradable because the degradabilities for all vessels were less than 60% (Test Methods and Judgment Criteria for the Designation of Monitoring Chemical Substances, April 12, 2010).