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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 906-936-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics in vivo
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Publication, meets generally accepted scientifically principles
- Justification for type of information:
- Read across is based on the category approach. Please refer to attached category document.
Data source
Reference
- Reference Type:
- publication
- Title:
- Inhibition of Metabolism of Diethylene Glycol Prevents Target Organ Toxicity in Rats
- Author:
- Lauren M. Besenhofer, Patrick A. Adegboyega, Michael Bartels, Mark J. Filary, Adam W. Perala, Marie C. McLaren and
Kenneth E. McMartin
- Year:
- 2 010
- Bibliographic source:
- TOXICOLOGICAL SCIENCES 117(1), 25–35 (2010)
Materials and methods
- Objective of study:
- other: metabolism and toxicity
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Rats were treated by oral gavage with water, 2 g/kg bw DEG (low dose), 10 g/kg bw DEG (high dose), or 10 g/kg bw DEG + fomepizole, and blood and urine were collected over 48 h to investigate the mechanism for the acute toxicity of DEG, and the effect of the alcohol dehydrogenase inhibitor 4-methylpyrazole (fomepizole), by determining the relationship between accumulation of DEG or its metabolites and the resulting kidney and liver toxicity.
- GLP compliance:
- no
Test material
- Reference substance name:
- 2,2'-oxydiethanol
- EC Number:
- 203-872-2
- EC Name:
- 2,2'-oxydiethanol
- Cas Number:
- 111-46-6
- Molecular formula:
- C4H10O3
- IUPAC Name:
- 2,2'-oxydiethanol
- Test material form:
- not specified
- Details on test material:
- - Supplier: Shell Chemicals
- Analytical purity: 99.78 %
- Impurities (identity and concentrations): EG (0.05 %), and triethylene glycol (0.08 %)
Constituent 1
- Radiolabelling:
- no
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Harlan, indianapolis, IN
- Age at study initiation: adult animals were used
- Weight at study initiation: 425–475 g
- Housing: in metabolic cages for 48 h for urine collection
- Individual metabolism cages: yes
- Diet: ad libitum: yes
- Water: ad libitum: yes
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 25 ± 2
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- not specified
- Details on exposure:
- no data
- Duration and frequency of treatment / exposure:
- - DEG was administered once by oral gavage at time 0
- Fomepizole (15 mg/kg bw) was administered ip as a 10 mg/ml solution in saline at 15 min and then at 12, 24, and 36 h (10 mg/kg bw) for a total of four ip doses
Doses / concentrations
- Remarks:
- Doses / Concentrations:
- 2 g/kg bw or 10 g/kg bw DEG
- 10 g/kg bw DEG + Fomepizole (15 mg/kg bw)
- No. of animals per sex per dose / concentration:
- Six male rats per group
- Control animals:
- yes
- Positive control reference chemical:
- no
- Details on study design:
- The purpose of this study was to investigate the mechanism for the acute toxicity of DEG, and the effect of the alcohol dehydrogenase inhibitor 4-methylpyrazole (fomepizole), by determining the relationship between accumulation of DEG or its metabolites and the resulting kidney and liver toxicity.
- Details on dosing and sampling:
- - Urine was collected at 4, 8, 12, 24, 36, and 48 h over ice to minimize degradation of urinary metabolites
- Approximately 1 mL of blood was collected via the indwelling jugular catheter at 4, 8, 12, 24, 36, and 48 h into heparinized syringes - Statistics:
- Differences between treatment groups and time points were assessed with two-way ANOVA with Bonferroni post-hoc test. To compare differences between treatment groups only, one-way ANOVA with Tukey post-hoc test was used. All analyses were performed using GraphPad Prism 5 for Windows. Tests were considered significant if p < 0.05.
Results and discussion
- Preliminary studies:
- Not applicable
Toxicokinetic / pharmacokinetic studies
- Details on absorption:
- Not applicable
- Details on distribution in tissues:
- Not applicable
- Details on excretion:
- Not applicable
Metabolite characterisation studies
- Metabolites identified:
- yes
- Details on metabolites:
- After low and high doses of DEG, 2-hydroxyethoxyacetic acid (HEAA) was the primary metabolite in the urine, with only minor amounts of urinary diglycolic acid (DGA). Small amounts of ethylene glycol (EG), but not oxalate or glycolate, were observed in the urine.
Bioaccessibility (or Bioavailability)
- Bioaccessibility (or Bioavailability) testing results:
- Not applicable
Any other information on results incl. tables
Rats treated with highdose DEG had metabolic acidosis, increased blood urea nitrogen and creatinine, and marked kidney necrosis, noted by histopathology. A minor degree of liver damage was noted at the high dose.
Treatment with fomepizole blocked the formation of HEAA and DGA and the development of metabolic acidosis and the kidney and liver toxicity.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.