4-cyclohexylphenol

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Umweltbundesamt Berlin - Verfahrensvorschlag (F.1.1) "Letale Wirkung beim Zebrabärbling Brachyodanio rerio" (Lethal effects on Brachydanio rerio) (LC0, LC50, LC100, 48-96h)

Version / remarks:

01.06.82

GLP compliance:

no

Remarks:

Study conducted prior to GLP implementation

Test material

Test material information

Constituent 1

Reference substance name:

4-cyclohexylphenol

EC Number:

214-465-4

EC Name:

4-cyclohexylphenol

Cas Number:

1131-60-8

Molecular formula:

C12H16O

IUPAC Name:

4-cyclohexylphenol

Test solutions

Vehicle:

yes

Remarks:

prediluted in acetone

Details on test solutions:

For the preparation of the stock solution the substance (1g) has been pre-diluted in Acetone (1g) and taken out under stirring with Ultra-Turrax.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Length at study initiation (length definition, mean, range and SD): 30 ± 5 mm

FEEDING DURING TEST
- No feeding during test

Study design

Water media type:

freshwater

Total exposure duration:

96 h

Test conditions

Hardness:

15° dH

Test temperature:

23 ± 2 °C

pH:

7.0 ± 0.2

Nominal and measured concentrations:

1000 mg/L (emuslified)

Details on test conditions:

TEST SYSTEM
- Test vessel: Aquarium
- Material, size, headspace, fill volume: fill volume 8.4L, but filled only with 3.5L water
- Aeration: 3 bubbles per second
- No. of organisms per vessel: 10 per 3.5 L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted, demineralised drinking water (by activated carbon & ion exchanger to eliminate Cu)
- Ca/Mg ratio: 4:1

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

7.5 mg/L

Conc. based on:

test mat.

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

8 mg/L

Conc. based on:

test mat.

Details on results:

LC50 could not be determined due to the steep dose/response curve.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

LC100 (96h) = 8 mg/L
LC0 (96h) = 7.5 mg/L;
LC50 could not be determined due to the steep dose/response curve, but will be ~ 7.7 mg/L. This triggers a classification as Category Chronic 2 as there are no chronic data available and the substance is not readily biodegradable and the logPow > 4.