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EC number: 226-615-6 | CAS number: 5437-98-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well performed GLP and OECD guideline study. As compared to actual guidelines a differing set of tester strains was used.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- as valid in 1996
- Deviations:
- yes
- Remarks:
- As compared to actual guidelines a differing set of tester strains was used.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 4'-methoxyacetoacetanilide
- EC Number:
- 226-615-6
- EC Name:
- 4'-methoxyacetoacetanilide
- Cas Number:
- 5437-98-9
- Molecular formula:
- C11H13NO3
- IUPAC Name:
- N-[4-(methoxyacetyl)phenyl]acetamide
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver preparation and co-factors (S9 mix)
- Test concentrations with justification for top dose:
- 33, 100, 333, 1000, 3333, 10000 µg per plate
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (2-AAN), sodium azide (NaN3), 9-aminoacridine (9-AA) and 2-nitrofluorene (2-NF)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: plate incorporation test
DURATION
- Exposure duration: the plates were inverted and incubated at 37°C for 2 days
NUMBER OF REPLICATIONS: Two independent mutation tests were conducted. Triplicate plates were prepared for each bacterial strain and dose level in both the presence and absence of S9 mix.
DETERMINATION OF CYTOTOXICITY: A toxicity test using strain TA 100 only was performed in the presence and absence of S9 mix
- Method: background lawn of microcolonies, reduction in mutant colony number - Evaluation criteria:
- A significant mutagenic response was recorded if there was:
I) for S. typhimurium strains TA 1535, TA 1537, TA 1538 and TA 98, at
least a doubling of the mean concurrent vehicle control values at some
concentration of the test substances and, for S. typhimurium strain
TA 100, a 1.5-fold increase over the control value. If the mean colony
count on the vehicle control plates was less than 10 then a value of 10
was assumed for assessment purposes. In such cases a minimum count of
20 was required before a significant mutagenic response was identified.
II) a dose related response, although at high dose levels this relationship
could be inverted because of, for example, (1) toxicity to the bacteria
generally, (2) specific toxicity to the mutants and (3) inhibition of foreign
compound metabolising enzymes where mutagens require metabolic
activation by the liver.
III) a reproducible effect in independent tests. - Statistics:
- mean values and standard deviation were generally calculated from triplicate plate counts
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- There was no toxicity to the background lawn of microcolonies, however there was a reduction in mutant colony number at 10000 µg per plate in some tester strains.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- There was no toxicity to the background lawn of microcolonies, however there was a reduction in mutant colony number at 10000 µg per plate in some tester strains.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
It was concluded that the test item was not mutagenic to Salmonella typhimurium when tested in dimethylsulphoxide up to a predetermined maximum limit. - Executive summary:
The test item was tested for mutagenic activity in Salmonella typhimurium strains TA 1535, TA 1537, TA 1538, TA 98 and TA 100 at concentrations ranging from 33 to 10000 µg per plate.
The tests were conducted on agar plates in the presence and absence of an Aroclor 1254 induced rat liver preparation and co-factors (S9-mix) required for mixed-function oxidase activity.
Concurrent positive controls demonstrated the sensitivity of the assay and the metabolising activity of the S9 mix.
The results obtained in both experiments were similar. No mutagenic activity was observed in any of the 5 bacterial strains, in either activation condition.
No precipitation of the test material was observed.
There was no toxicity to the background lawn of microcolonies, however there was a reduction in mutant colony number at 10000 µg per plate.
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