7-acetamido-4-hydroxy-3-[[4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2-sulphonic acid

Endpoint summary

Description of key information

Short term toxicity to aquatic invertebrate

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to invertebrate was predicted 7-acetamido-4-hydroxy-3-[(4-{[2-(sulfooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2-sulfonic acid (CAS: 68189-39-9). Intoxication value was estimated to be 631.974 mg/l for Daphnia magna for 48 hrs duration. It was concluded that 7-acetamido-4-hydroxy-3-[(4-{[2-(sulfooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2-sulfonic acid (CAS: 68189-39-9) was likely to be not toxic to aquatic invertebrate.

Toxicity to aquatic algae and cyanobacteria

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to algae was predicted 7-acetamido-4-hydroxy-3-[(4-{[2-(sulfooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2-sulfonic acid (CAS: 68189-39-9). Growth rate value was estimated to be 300.026  mg/l for Pseudokirchneriella subcapitata for 72 hrs duration. It was concluded that 7-acetamido-4-hydroxy-3-[(4-{[2-(sulfooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2-sulfonic acid (CAS: 68189-39-9) was likely to be not toxic to aquatic algae.

Additional information

Short term toxicity to aquatic invertebrate

Following studies include predicted data and the experimental studies for the structurally similar read across to conclude the toxicity extent of 7-acetamido-4-hydroxy-3-[(4-{[2-(sulfooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2-sulfonic acid (CAS: 68189-39-9) towards aquatic invertebrate is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to invertebrate was predicted 7-acetamido-4-hydroxy-3-[(4-{[2-(sulfooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2-sulfonic acid (CAS: 68189-39-9). Intoxication value was estimated to be 631.974 mg/l for Daphnia magna for 48 hrs duration. It was concluded that 7-acetamido-4-hydroxy-3-[(4-{[2-(sulfooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2-sulfonic acid (CAS: 68189-39-9) was likely to be not toxic to aquatic invertebrate.

The predicted data for the target chemical is supported by the experimental study of structurally similar read across disodium 5-acetamido-4-hydroxy-3-(phenyldiazenyl)naphthalene-2,7-disulfonate (CAS: 3734 -67 -6) from ABITEC reports suggests that To study the effects of Acid Red 1 (Red 2G); disodium 5-acetamido-4-hydroxy-3-(phenyldiazenyl)naphthalene-2,7-disulfonate on aquatic invertebrate test was carried out according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test).

The solution 100 mg/L was prepared by dissolving red powder in reconstituted water. The solution was kept 30 min in ultrasonic bath.

The study was carried out with 5 daphnia magna per vessel under static condition at temp 18°C, pH of 8.2, dissolved oxygen 7.6 mg/L and above at end of test, the inhibition of mobility of daphnids was determined during the test.

The effective concentration EC50 to 50% of daphnia magna for Disodium 5-acetylamino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate in 48 hr is 100 mg/L. It can be concluded from the value that the Disodium 5-acetylamino-4-hydroxy-3-(phenylazo)naphthalene-2,7-disulphonate is not toxic to the aquatic invertebrate and can be considered as "not classified" as per the classification crietria fro aquatic environment.

Similar experimental study of another structurally similar read acrossTetrasodium 4-amino-5-hydroxy-3,6-bis[[4-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonate (CAS: 17095 -24 -8) from Authoritative database J-Check also assists that the Short-term toxicity to aquatic invertebrates test was carried out according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)to study the effects ofTetrasodium 4-amino-5-hydroxy-3,6-bis[[4-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonate on aquatic invertebrate.

Mobility of 50% population of daphnia magna was measured during the test. The effective concentration EC50 to 50% population of daphnia magna for Tetrasodium 4-amino-5-hydroxy-3,6-bis[[4-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonate on aquatic invertebrate in 48 hr is >1000 mg/L. It can be concluded from the value that the Tetrasodium 4-amino-5-hydroxy-3,6-bis[[4-[[2-(sulphonatooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonate is not toxic to the aquatic invertebrate and can be considered as “not classified” as per the classification criteria for aquatic environment.

Thus based on the effect concentrations which is in range of 100 mg/l to >1000 mg/L gives the conclusion that test substance 7-acetamido-4-hydroxy-3-[(4-{[2-(sulfooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2-sulfonic acid (CAS: 68189-39-9) was likely to be not toxic to invertebrate at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be“not classified”as per the CLP classification criteria for aquatic environment.

Toxicity to aquatic algae and cyanobacteria

Following studies include predicted data and the experimental studies for the structurally similar read across to conclude the toxicity extent of 7-acetamido-4-hydroxy-3-[(4-{[2-(sulfooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2-sulfonic acid (CAS: 68189-39-9) towards aquatic algae is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity to algae was predicted 7-acetamido-4-hydroxy-3-[(4-{[2-(sulfooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2-sulfonic acid (CAS: 68189-39-9). Growth rate value was estimated to be 300.026  mg/l for Pseudokirchneriella subcapitata for 72 hrs duration. It was concluded that 7-acetamido-4-hydroxy-3-[(4-{[2-(sulfooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2-sulfonic acid (CAS: 68189-39-9) was likely to be not toxic to aquatic algae.

The predicted data for the target chemical is supported by the experimental study of structurally similar read across disodium 5-acetamido-4-hydroxy-3-(phenyldiazenyl)naphthalene-2,7-disulfonate (CAS: 3734 -67 -6) from ABITEC reports suggests that The predicted data for the target chemical is supported by the experimental study of structurally similar read across disodium 5-acetamido-4-hydroxy-3-(phenyldiazenyl)naphthalene-2,7-disulfonate (CAS: 3734 -67 -6) from ABITEC reports suggests thatA freshwater algal growth inhibition exposure assessment was used to test how a range of concentrations of CAS 3734-67-6 exerts different degrees of toxic effects on the growth of Desmodesmus subspicatusunder otherwise identical test conditions. The test was performed in close resemblance to OECD guideline 201 by ABITEC in Prague, Crez Republic. The standardized protocol used to obtain the herein described results was by the testing laboratory referred to as “CSN EN ISO 8692”. The 72 hours EC50 was by the testing laboratory defined as the concentration estimated to inhibit 50 per cent of the algae cell growth compared to unexposed cells after 27 hours of exposure. The nominal concentrations used for the exposure assessment were as follows:0, 20, 30, 45, 67 and 100 mg/L. The positive control/reference substance used in the tested showed an expected result and gave a EC50 that corresponded to previous exposures with this chemical in Desmodesmus subspicatus.In conclusion, the EC50 for CAS 3734-67-6 was 285.8 mg/L (95% C.I.: 173.5-470.8 mg/L) after 72 hours of exposure.It can be concluded fom the value that the disodium 5-acetamido-4-hydroxy-3-(phenyldiazenyl)naphthalene-2,7-disulfonate is not toxic to aquatic algae and can be considered as "not classified" as per the classification criteria for aquatic environment.

Another exeprimental study of structurally similar read across 1,2-dihydropyridazine-3,6-dione, monopotassium salt (CAS: 28382 -15 -2) from U.S. Environmental Protection Agency 1992, also suggests that the Toxicity to aquatic algae and cyanobacteria test as carried out for 1,2-dihydropyridazine-3,6-dione, monopotassium salt for 5 days to study the effects on aquatic algae. Population (Abundance) was measured during the test. The study was carried out with 309 Skeletonema costatum

(Diatom) in saltwater under static condition. The Effective concentration EC50 to 50% of Skeletonema costatum for 1,2-dihydropyridazine-3,6-dione, monopotassium salt in 5 days is 102 mg/L. It can be concluded from the value that the 1,2-dihydropyridazine-3,6-dione, monopotassium salt is not toxic to the aquatic algae and can be considered as “not classified” as per the classification criteria for aquatic environment.

Thus based on the effect concentrations which is in range of 102 mg/l to 300.026 mg/L gives the conclusion that test substance 7-acetamido-4-hydroxy-3-[(4-{[2 (sulfooxy)ethyl]sulfonyl}phenyl)diazenyl]naphthalene-2-sulfonic acid (CAS: 68189-39-9) was likely to be not toxic to algae at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be“not classified”as per the CLP classification criteria for aquatic environment.