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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
July 07, 1993
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
- Deion. water instead of dest. water for the stocksolution; - Preparation of the inoculum : higher sludge weight after the washing procedure (Sludge concentrations in the test according to the guidelines); - 4 instead of 5 test concentrations
Principles of method if other than guideline:
Internal method: SAV: 05 000 02
GLP compliance:
yes
Analytical monitoring:
not specified
Details on sampling:
- Concentrations: 320, 100, 32 and 10 mg/l of the test substance
Vehicle:
not specified
Test organisms (species):
activated sludge
Details on inoculum:
Test system / Test organism
- Organism: Activated sludge of a communal sewage treatment plant (ARA-Birsig Therwil)
- Sludge pretreatment : 3 times washing with tap water and addition of synthetic sewage (a day before the main test)
Test type:
not specified
Water media type:
not specified
Limit test:
no
Total exposure duration:
3 h
Test temperature:
20.5 °C
pH:
7.2 before the test beginning
Details on test conditions:
Experimental conditions
- Sludge dry weight: 1.56 g/l
- Test volume: 100 ml
- Estimation technique: O2 - consumption, measured with an oxygen sensitive electrode system: OXI DIGI 530 (SAV : 00 109)
- Aeration: 0.1 - 0.2 l/min by pasteur-pipette
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 320 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
The IC50 (3 h) of the reference substance 3,5-Dichlorophenol was 8.8 mg/l.
Validity criteria fulfilled:
not specified
Conclusions:
IC50 > 320 mg/l
Executive summary:

Method:


The toxicity to microorganisms of the test substance was evaluated according to the OECD guideline 209. The test substance concentrations tested were 320, 100, 32 and 10 mg/l. The reference substance, 3,5-Dichlorophenol was tested in concentrations of 32, 10 and 3.2 mg/l.


 


Conclusion:


IC50 (3 h) of the test substance > 320.0 mg/l


IC50 (3 h) of the reference substance 3,5-Dichlorophenol = 8.8 mg/l

Description of key information

IC50 > 320 mg/l

Key value for chemical safety assessment

EC50 for microorganisms:
320 mg/L

Additional information

The toxicity to microorganisms of the test substance was evaluated according to the OECD guideline 209. The test substance concentrations tested were 320, 100, 32 and 10 mg/l. The reference substance, 3,5-Dichlorophenol was tested in concentrations of 32, 10 and 3.2 mg/l.


 


This result is in line with the one obtained with other tests listed in the table attached below.