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EC number: 221-816-5 | CAS number: 3244-88-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from authoritative databases
Data source
Referenceopen allclose all
- Reference Type:
- other: Authoritative database
- Title:
- Hazardous Substances Data Bank (HSDB) for Methanesulfonic acid
- Author:
- U.S National Library of Medicine
- Year:
- 2 010
- Bibliographic source:
- Hazardous Substances Data Bank (HSDB) for Methanesulfonic acid, U.S National Library of Medicine, 2010
- Reference Type:
- secondary source
- Title:
- High Production Volume Information System (HPVIS) for Methanesulfonic acid (75-75-2)
- Author:
- USEPA
- Year:
- 2 009
- Bibliographic source:
- High Production Volume Information System (HPVIS) for Methanesulfonic acid (75-75-2); Available from November 20, 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Buehler test
- Principles of method if other than guideline:
- To assess the skin sensitization potential of Methanesulfonic acid on guinea pig skin according to Buehler method
- GLP compliance:
- no
- Type of study:
- Buehler test
- Justification for non-LLNA method:
- not specified
Test material
- Reference substance name:
- Methanesulphonic acid
- EC Number:
- 200-898-6
- EC Name:
- Methanesulphonic acid
- Cas Number:
- 75-75-2
- Molecular formula:
- CH4O3S
- IUPAC Name:
- methanesulfonic acid
- Test material form:
- liquid
- Details on test material:
- Name of the test chemical: Methanesulfonic acid
Molecular Formula:CH4O3S
Molecular Weight: 96.1056 g/mol
InChI: 1S/CH4O3S/c1-5(2,3)4/h1H3,(H,2,3,4)
Substance Type: Organic
Physical State:Liquid
Constituent 1
- Specific details on test material used for the study:
- Name of the test chemical: Methanesulfonic acid
Molecular Formula:CH4O3S
Molecular Weight: 96.1056 g/mol
InChI: 1S/CH4O3S/c1-5(2,3)4/h1H3,(H,2,3,4)
Substance Type: Organic
Physical State:Liquid
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- Hartley
- Sex:
- male/female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Harlan Spragu e Dawley , Indiana
- Age at study initiation: No Data available
- Weight at study initiation:
males: 429.6 ± 18.8g
females: 400.5 ± 27.6g
Study design: in vivo (non-LLNA)
Induction
- Route:
- epicutaneous, open
- Vehicle:
- water
- Concentration / amount:
- Concentration :50%
Amount : 0.3 ml - Day(s)/duration:
- 6 hours
- Adequacy of induction:
- not specified
Challenge
- No.:
- #1
- Route:
- epicutaneous, open
- Vehicle:
- water
- Concentration / amount:
- 25%
- Day(s)/duration:
- 24 hours
- Adequacy of challenge:
- not specified
- No. of animals per dose:
- 20
- Details on study design:
- RANGE FINDING TESTS: Yes
A preliminary study was conducted in order to determine the concentrations to be tested in the main study. The irritation potential of methane sulfonic acid 70% at levels of
undiluted, 50%, 25%, 10%, 5%, 2.5%, 1% and 0.5% was evaluated in two groups of four animals each. Four levels of test material were evaluated per animals. Dilutions were obtained with distilled water (w/v). 0.3 ml of different solutions was applied into a 25 mm Hill Top Chamber, which were placed on animals clipped back for 6 hours. The day after, animals were depilat ed and two hours later, examined for irritation, according the following scale. Another score was performed 40h after exposure.
Grading scheme for Pilot Study
-Grading system:
0: no reaction
±: slight, patch y erythema
1: slight but confluent or m oderate
patchy erythema
2: moderate erythema
3: severe erythema wi th or without oedema
MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures: 3
- Exposure period: 6 hours
- Test groups: 5 males + 5 females
- Control group: 5 males + 5 females
- Site: left shoulder
- Frequency of applications: Thrice in 14 days
- Duration: 14 days
- Concentrations: 50%-0.3ml
B. CHALLENGE EXPOSURE
- No. of exposures: 1
- Day(s) of challenge: 1 (on day 28)
- Exposure period: 24 hrs
- Test groups: 5 males + 5 females
- Control group: 5 males + 5 females
- Site: right flank
- Concentrations: 25%-0.3ml
- Evaluation (hr after challenge):24hrs - Challenge controls:
- vehicle control used
- Positive control substance(s):
- not specified
Results and discussion
- Positive control results:
- no data available
In vivo (non-LLNA)
Results
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 25%
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Clinical observations:
- no data available
- Remarks on result:
- no indication of skin sensitisation
Any other information on results incl. tables
RESULTS OF PILOT STUDY:
Undiluted methane sulfonic acid 7 0% produced grades of 2, 1 and ±, with oedema, blanching and scabbing on two sites. 50%, 25%, 10%, 5%, 2.5% and 1% produced grades of ±, while 0.5% produced grades of ± and 0. Consequently, the 50% concentration was chosen for use at induction for the test group, since it caused no greater than mild to moderate primary irritation. Moreover, the 25% concentration was selected for challenge, because it caus ed no more than slight irritation.
RESULTS OF TEST
-Sensitization reaction: None of the test animals responded with a skin grade that would have been suggestive of sensitization
Applicant's summary and conclusion
- Interpretation of results:
- other: Not sensitizing
- Conclusions:
- 20 guinea-pigs were induced by 50% of methane sulfonic acid 70% solution (i.e. 35% of methane sulfonic acid) and challenged by 25% of methane sulfonic acid 70% solution (i.e. 17.5% of methane sulfonic acid). None of the test animals responded with a skin grade that would have been suggestive of sensitization.
Hence, methane sulphonic acid can be considered to be not sensitizing to the skin of Hartley guinea pigs. - Executive summary:
The dermal sensitization potential of Methanesulfonic acid was assessed in guinea pigs according to Buehler method.
The irritation potential of methane sulphonic acid 70% at levels of undiluted, 50%, 25%, 10%, 5%, 2.5%, 1% and 0.5% was evaluated in two groups of four animals each prior to the sensitization study.The 50% concentration from the preliminary study was chosen for use at induction for the test group, since it caused no greater than mild to moderate primary irritation. Moreover, the 25% concentration was selected for challenge, because it caused no more than slight irritation.
20 Hartley guinea pigs (10/sex) were induced by 50% of methane sulphonic acid. On day 1, dermal application of 0.3 ml of test substance (treated group) or with the vehicle (control group) was performed on the left shoulder. On day 7, the same region received another topical application. On day 14, this same site was treated by a last topical application. All these applications lasted approximately 6 hours. On day 28, all the animals were challenged with 0.3 ml of the test substance at the concentration of 25% in their right flank. The challenge exposure lasted for 24 hours. Water served as vehicle control and 5guinea pigs/sex were used in the control group. The dermal reactions were observed and scored 24 hours after the challenge exposure.
20 guinea-pigs were induced by 50% of methane sulphonic acid 70% solution/ (i.e. 35% of methane sulphonic acid) and challenged by 25% of methane sulphonic acid 70% solution (i.e. 17.5% of methane sulphonic acid). None of the test animals responded with a skin grade that would have been suggestive of sensitization.
Hence, methane sulphonic acid can be considered to be not sensitizing to the skin of Hartley guinea pigs.
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