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EC number: 204-541-5 | CAS number: 122-40-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
The mutagenicity effect of α-Amylcinnamaldehyde was studied by performing experiment in Salmonella typhimurium. Salmonella typhimurium stains TA1535, TA100, TA1537, TA1538, TA98 were used in study. Mutagenic assay was performed by plate incorporation method with and without metabolic activation i.e. S9 mix prepared from liver fraction of Aroclor pretreated rats (Aroclor 1254). DMSO used as solvent for test substance. Five doses of each substance were tested (up to 3.6 mg/plate) in all five tester strains Vogel Bonnet medium was used throughout; plates were incubated for 48 hr. Positive controls were run in each experiment with the reference mutagens sodium azide and benzo[a]pyrene. Test substance was tested at least twice.Test substance did not induce mutation in the tester strains, therefore α-Amylcinnamaldehyde was estimated to be not mutagenic in Salmonella typhimurium strains.
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- No data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Peer reviewed journal research article
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Deviations:
- not specified
- Principles of method if other than guideline:
- The mutagenicity potential of α-Amylcinnamaldehyde by conducting experiment in Salmonella typhimurium TA1535, TA100, TA1537, TA1538 and TA 98.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): α-Amylcinnamaldehyde
- Molecular formula (if other than submission substance): C14H18O
- Molecular weight (if other than submission substance): 202.2952 g/mol
- Substance type: Organic
- Physical state: No data
- Purity: No data
- Impurities (identity and concentrations): No data - Target gene:
- Histidine
- Species / strain / cell type:
- other: Species: Salmonella typhimurium Strain: TA1535, TA100, TA1537, TA1538, TA98
- Details on mammalian cell type (if applicable):
- Not applicable.
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mixture from liver fraction of rat pretreated with Aroclor 1254
- Test concentrations with justification for top dose:
- Up to 3.6 mg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: Solubility of test substance in DMSO solvent. - Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- benzo(a)pyrene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: Plate incorporation
DURATION
- Preincubation period: No data
- Exposure duration: 48 hrs
- Expression time (cells in growth medium): No data
- Selection time (if incubation with a selection agent): No data
- Fixation time (start of exposure up to fixation or harvest of cells): No data
SELECTION AGENT (mutation assays): No data
SPINDLE INHIBITOR (cytogenetic assays): No data
STAIN (for cytogenetic assays): No data
NUMBER OF REPLICATIONS: Two
NUMBER OF CELLS EVALUATED: No data
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: No data
OTHER EXAMINATIONS:
- Determination of polyploidy: No data
- Determination of endoreplication: No data - Rationale for test conditions:
- No data
- Evaluation criteria:
- Dose dependent increase in number of revertants.
- Statistics:
- Kastenbaum and Bowman Method
- Species / strain:
- other: Species: Salmonella typhimurium Strain: TA1535, TA100, TA1537, TA1538, TA98
- Metabolic activation:
- with and without
- Genotoxicity:
- not specified
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not valid
- Positive controls validity:
- valid
- Additional information on results:
- No data
- Remarks on result:
- other: No mutagenic effect were observed
- Conclusions:
- The mutagenicity potential of α-Amylcinnamaldehyde was evaluated by performing mutagenicity assay in Salmonella typhimurium strains TA1535, TA100, TA1537, TA1538, TA98, test substance did not induce mutation in all bacterial tester strains. Therefore α-Amylcinnamaldehyde was considered to be not mutagenic in Salmonella typhimurium strains.
- Executive summary:
The mutagenicity effect of α-Amylcinnamaldehyde was studied by performing experiment in Salmonella typhimurium. Salmonella typhimurium stains TA1535, TA100, TA1537, TA1538, TA98 were used in study. Mutagenic assay was performed by plate incorporation method with and without metabolic activation i.e. S9 mix prepared from liver fraction of Aroclor pretreated rats (Aroclor 1254). DMSO used as solvent for test substance. Five doses of each substance were tested (up to 3.6 mg/plate) in all five tester strains Vogel Bonnet medium was used throughout; plates were incubated for 48 hr. Positive controls were run in each experiment with the reference mutagens sodium azide and benzo[a]pyrene. Test substance was tested at least twice.
Test substance did not induce mutation in the tester strains, therefore α-Amylcinnamaldehyde was considered to be not mutagenic in Salmonella typhimurium strains.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Based on the various experimental data for the target chemical have been reviewed to determine the mutagenic nature of alpha-Amyl cinnamaldehyde. The studies are as mentioned below:
Based on the experimental key study conducted by Wild, et.al. (Food and chemical toxicology, 1983) .The mutagenicity effect of α-Amylcinnamaldehyde was studied by performing experiment in Salmonella typhimurium stains TA1535, TA100, TA1537, TA1538, TA98. Mutagenic assay was performed by plate incorporation method with and without metabolic activation i.e. S9 mix prepared from liver fraction of Aroclor pretreated rats (Aroclor 1254). DMSO used as solvent for test substance. Five doses of each substance were tested (up to 3.6 mg/plate) in all five tester strains Vogel Bonnet medium was used throughout; plates were incubated for 48 hr. Positive controls were run in each experiment with the reference mutagens sodium azide and benzo[a]pyrene. Test substance was tested at least twice. Test substance did not induce mutation in the tester strains, therefore α-Amylcinnamaldehyde was considered to be not mutagenic in Salmonella typhimurium strains.
Similarly in the another study, where data is from authoritative database HSDB (2017) were observed . In vitro genetic toxicity study to Salmonella typhimurium species and strainsTA 1535, TA 100, TA 1537, TA 1538, TA 98 by using alpha-Amyl cinnamaldehyde in the presence of exogenous metabolic activation was prepared from Aroclor-pretreated rats (Aroclor 1254, 500 mg/kg).Toxicity checked at the maximum concentration. The test substance was exposed at the concentration of 3600 ug/plate .alpha-Amyl cinnamaldehyde did not induce a toxicity, dose-related increase in his+revertants over the corresponding solvent in theS. typhimurium tester strains TA 1535, TA 100, TA 1537, TA 1538, TA 98 in the presence of S9 metabolic activation system and hence is negative for mutation in vitro. Hence the test substance can not be classified as gene mutant in vitro.
Similarly another experimental study was performed were data is from handbook and collection databases (HSDB, 2016 and HPVIS, 2017). In vitro genetic toxicity study toSalmonella typhimurium species and strainsTA1535, TA100, TA1537, TA98 by using alpha-Amyl cinnamaldehyde in the absence and presence of exogenous metabolic activation was prepared from Aroclor-pretreated rats (Aroclor 1254, 500 mg/kg).Toxicity checked at the maximum concentration. Solvent control, DMSO was also run simultaneously.Based on the dose concentration 2.5 to 750 µg/plate without activation and 250 µg/plate with activation, alpha-Amyl cinnamaldehydedid not induce a toxicity, dose-related increase in his+revertants over the corresponding solventin theS. typhimurium tester strains TA1535, TA100, TA1537, TA98 in the presence of S9 metabolic activation system and in the absence .Hence is negative for mutation in vitro.
Similarly another studies was performed were data is from handbook and collection data (HSDB, 2016 and HPVIS, 2017). In vitro genetic toxicity study on Salmonella typhimurium species and strainsTA97 and TA102 by using alpha-Amyl cinnamaldehyde in the absence and presence of exogenous metabolic activation was prepared from Aroclor-pretreated rats (Aroclor 1254, 500 mg/kg).Toxicity checked at the maximum concentration. 9-aminoacridine was taken as a positive control for TA97 (with and without) and TA102 without activation, positive control for TA102 without S9 was mitomycin C.Based on the above concentration 1-1000 µg/plate alpha-Amyl cinnamaldehyde did not induce a toxicity, dose-related increase in his+revertants over the corresponding solvent in the S. typhimurium tester strains TA97 and TA102 in the presence of S9 metabolic activation system and absence hence is negative for mutation in vitro.
Similarly study was conducted by Hiroshi Fujita and Mieko Sasaki(Annual Report of Tokyo Metropolitan Research Laboratory of Public Health, 1987). The Mutagenic potential of α-Amyl Cinnamaldehyde was evaluated by conducting experiment in Salmonella typhimurium StrainsTA97, TA102 with and without metabolic activation i.e. S9 mix. Mutagenic assay was performed by Preincubation method describe by Ames.DMSO was used as vehicle for test substance. Test chemical in doses0, 0.001, 0.005, 0.01, 0.5, 1 mg/plate were studied in mutagenic assay.Test chemical was preincubated with S9 mix for 20 min. Positive control and vehicle control also run parallel to test chemical. The number of revertants in test chemical treated plates were less than solvent control as dose dependent increase in number of revertants not reported hence test chemical did not induce mutation. Therefore alpha -Amyl Cinnamaldehyde was not mutagenic in Salmonella typhimurium.
Based on the data available for the target chemical α-Amyl Cinnamaldehyde does not exhibit gene mutation in vitro. Hence the test chemical is not likely to classify as a gene mutant in vitro as per the criteria mentioned in CLP regulation.
Justification for classification or non-classification
Based on the data available for the target chemical α-Amyl Cinnamaldehyde does not exhibit gene mutation in vitro. Hence the test chemical is not likely to classify as a gene mutant in vitro as per the criteria mentioned in CLP regulation.
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