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EC number: 435-580-8 | CAS number: 56553-60-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004-07-14 - 2004-11-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline study according GLP
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hessisches Ministerium für Umwelt, ländliche Raum und Umweltschutz, 2002
- Type of assay:
- bacterial reverse mutation assay
- Target gene:
- Salmonella typhiumurium: his
E. coli: trp - Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix prepared form Phenobarbital/beta-Naphtoflavone induced rat liver
- Test concentrations with justification for top dose:
- Experiment I: 3, 10, 33, 100, 333, 1000, 2500 and 5000 µg/plate
Experiment II: 33, 100, 333, 1000, 2500 and 5000 µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: Based on its solubility properies and relative non-toxicity to bacteria. - Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- methylmethanesulfonate
- other: 4-nitro-o-phenylene-diamine; 2-aminoanthracene
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation); preincubation
DURATION
- Preincubation period: 60 min, 37°C
- Exposure duration: at least 48 h, 37°C
SELECTION AGENT (mutation assays): Ampicillin, 25 µg/mL
NUMBER OF REPLICATIONS: 3/concentration
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth - Evaluation criteria:
- A test item was considered as a mutagen if a biological relevant increase in the number of revertants exceeding the threshold of twice (TA 98, TA100, WPA2) uvrA) or three times (TA 1535 and TA1537) the colony number of the solvent control as observed.
A dose dependent increase was considered biological relevant if the threshold exceeded at more than one concentration.
An increase exceeding the threshold at only one concentration is judged as biological relevant if reproduced in an independent second experiment.
A dose dependent increase in the number of revertant colonies below the threshold was regarded as an indication of a mutagenic potential if reproduced in an independent second experiment. However, whenever the colony number remained within historical range of negative and solvent controls such an increase was not considered biologically relevant. - Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
- Conclusions:
- Interpretation of results (migrated information):
negative
Sodium triacetoxyborohydride did not induce an increase in the number of revertanst in Salmonella typhimurium and Escherichia coli upt o concentration of 5000 µg/plate. Thus it was found to be non mutagenic under conditions of this test. - Executive summary:
Genetic toxicity of Sodium triacetoxyborohydride was tested in a GLP study performed according OECD 471, where Salmonella typhimurium strains TA1535, TA1537, TA98 and TA98 and Escherichia coli WP2 uvrA were treated with substance concentration of 3, 10, 33, 100, 333, 1000, 2500 and 5000 µg/plate in experiment I and concentrations of 33, 100, 333, 1000, 2500 and 5000 µg/plate in experiment II with and without metabolic activation, respectively. As no increase in number of revertants was noted in all strains and at all concentrations, Sodium triacetoxyborohydride was found to be non mutagenic under conditions of this test.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
Genetic toxicity of Sodium triacetoxyborohydride in bacteria was tested in a GLP study performed according OECD 471, where Salmonella typhimurium strains TA1535, TA1537, TA98 and TA98 and Escherichia coli WP2 uvrA were treated with substance concentration of 3, 10, 33, 100, 333, 1000, 2500 and 5000 µg/plate in experiment I and concentrations of 33, 100, 333, 1000, 2500 and 5000 µg/plate in experiment II with and without metabolic activation, respectively. As no increase in number of revertants was noted in all strains and at all concentrations, Sodium triacetoxyborohydride was found to be non mutagenic under conditions of this test.
Sodium triacetoxyborohydride was tested in a GLP study performed according to OECD 473, where V79 cells were treated with concentrations up to 2150 µg/mL dissolved in DMSO for 4, 18 and 28 h without metabolic activation and for 4 h with metabolic activation (S9 mix), respectively. Since no structural chromosome aberrations were induced in V79 cells when treated up to 2150 µg/mL with and without metabolic activation, while the positive controls EMS and CPA did, Sodium triacetoxyborohydride was found to be non-clastonic under conditions of this test.
Boric acid was tested for inducing gene mutation in mammalian cells in a GLP study performed according to NTP protocol which is comparable to OECD guideline. Well maintained mouse lymphoma L5178Y cells were treated with boric acid up concentration of 5000 µg/mL with and without metabolic activation by a S9 mix for 4 hours. Since no toxicity and no increase in frequency was observed, boric acid was regarded to be non-mutagenic in mammalian cells.
Justification for selection of genetic toxicity endpoint
Reliable OECD guideline study according GLP
Justification for classification or non-classification
The available data on genetic toxicity of the test substance do not meet the criteria for classification according to Regulation (EC) 1272/2008, and are therefore conclusive but not sufficient for classification.
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