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EC number: 812-957-9 | CAS number: 333350-60-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-methoxy-N-methyl-3-nitrobenzamide
- EC Number:
- 812-957-9
- Cas Number:
- 333350-60-0
- Molecular formula:
- C9H10N2O4
- IUPAC Name:
- 4-methoxy-N-methyl-3-nitrobenzamide
- Test material form:
- solid: particulate/powder
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Determination of the test item
The limit concentration and the control were analytically verified via HPLC-DAD in fresh media at the start of the exposure (0 hours) and in old media at the end of the exposure (48 hours). The method was validated prior to this study according to SANCO 3029/99 rev.4 (2000).
Sampling for the analytical
At the start of the exposure (0 hours), samples of the fresh media were taken after preparation of the limit concentration and analyzed. At the end of the exposure (48 hours), samples of the old media were taken from additional replicates, which were prepared with test media, but without daphnids. These additional replicates were incubated under test conditions until sampling.
Criteria for the analytical monitoring (target)
Recoveries of the test item should be within ± 20% of the nominal or initially measured concentration.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Limit concentration
A limit concentration of 100 mg/L of the test item was tested. The limit concentration is based on the results of a non-GLP preliminary range finding test.
Preparation of the test concentration
The limit concentration (100 mg/L test item were weighed out) was prepared with dilution water one day prior to the start of the exposure (at -25 hours). The limit concentration was treated with ultrasound for 1 hour at 40 °C and stirred thereafter with a magnetic stirrer at approximately 1100 rpm for 24 hours at room temperature.
Control
Dilution water without test item incubated under the same conditions as the test group
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Test system
Daphnia magna STRAUS (Clone 5)
Reason for the selectionof the test system
Daphnia magna is the preferred species in accordance with the
test guideline and is bred at the test facility.
Origin
Institut für Wasser-, Boden- und Lufthygiene (WaBoLu),
14195 Berlin, Germany
Breeder
Noack Laboratorien GmbH,
Käthe-Paulus-Str. 1, 31157 Sarstedt, Germany
Culture
In glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20 2 °C, in an incubator, 16 hours illumination; light intensity of max. 1500 lx
Culture medium
Elendt M4, according to OECD 202, Annex 3 (2004), modified to a total hardness of 160 to 180 mg CaCO3/L, is used.
Culture feeding
The culture daphnids were fed at least 5 times per week ad libitum with a mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata and Desmodesmus subspicatus, with an algae cell density of > 106 cells/mL. The algae were cultured at the test facility.
Origin of the food algae
Sammlung von Algenkulturen (SAG),
Pflanzenphysiologisches Institut der Universität Göttingen, Nikolausberger Weg 18, 37073 Göttingen, Germany
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- see any other information on material and methods incl. tables
- Test temperature:
- see any other information on material and methods incl. tables
- pH:
- see any other information on material and methods incl. tables
- Dissolved oxygen:
- see any other information on material and methods incl. tables
- Conductivity:
- see any other information on material and methods incl. tables
- Details on test conditions:
- Test method
The study was performed with a static test design.
Test duration
48 hours
Test vessels
Glass beakers (4 (ID) x 7 (H) cm), 50 mL capacity, loosely covered with watch glasses
Test volume
20 mL in each test replicate (see ‘Application’)
Dilution water
Same as culture medium
Number of daphnids and replicates
20 daphnids, divided into 4 replicates, each with 5 daphnids, were used for each loading level and the control.
Age of the daphnids
Less than 24 hours old daphnids from a healthy stock were used for at the start of the exposure the study. Juvenile daphnids were removed from the culture vessels at the latest 24 hours before the start of the exposure and discarded. The juveniles born within the following period of max. 24 hours preceding the exposure were used for the test. No first brood progeny was used for the test.
Acclimatization
Acclimatization of the daphnids was not necessary, because the dilution water was equivalent to the culture medium.
Application
20 g test solution per replicate was weighed out into each test vessel. This corresponds to 20 mL per test vessel. The daphnids were inserted with a small amount of dilution water by a pipette.
Test temperature (target)
18 - 22 °C, constant within ± 1 °C
Illumination (target)
Diffuse light, light intensity of max. 1500 lx
Photoperiod (target)
16/8 hours light/dark cycle
Feeding
The daphnids were not fed during the study.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- other: EC10/50/100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- other: EC10/50/100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- The percentage of immobility for the reference item potassium dichromate (SIGMA-ALDRICH, batch number MKBV0900V, purity 99.0%, CAS RN 7778-50-9) was determined after 24 hours from 2017 03-09 to 2017-03-10. For results of the most recent of the monthly performed reference tests, see Table 8.
EC50-Value (with 95% confidence limits) of the Reference Item Potassium dichromate based on nominal concentrations mg/L, (0 - 24 hours)
Current Study Valid Range
EC50 2.05 mg/L
0.6 - 2.4 mg/L, acc. to AQS P 9/2 (02/2000); clone 5
0.6 - 2.1 mg/L, acc. to OECD 202 (2004); clone A
95% confidence limits 2.00 - 3.71 mg/L - Reported statistics and error estimates:
- Methods of evaluation
The effects of the limit concentration were empirically derived from the observed immobilization rates.
Since the measured test item concentrations were within ± 20% of the nominal concentration, all effect concentrations (EC10 / 50 / 100) given were based on the nominal concentration of the test item 4-Methoxy-N-methyl-3-nitrobenzamide.
EC-values and statistical analyses for reference item
An EC50-value was calculated for the reference item by sigmoidal dose-response regression. The respective 95% confidence limits were calculated from the standard error and the t-distribution. All calculations were carried out from the best-fit values with the software GraphPad Prism5.
Software
All data were computer-processed and rounded for presentation. Consequently, minor variations may occur from the original figures if manual calculations based on the original figures are made subsequently. Calculations were made using the following software:
- GraphPad Prism5, GRAPHPAD SOFTWARE, INC.
- Excel, MICROSOFT CORPORATION
Any other information on results incl. tables
Immobilization Rates [%]after 24 and 48 hours ofExposure in the Definitive Test
(n = 20, divided into 4 replicates with 5 daphnids each)
Nominal test item concentration [mg/L] |
IMMOBILIZATION [%] |
|||||||||
24 hours |
48 hours |
|||||||||
Replicates |
Replicates |
|||||||||
1 |
2 |
3 |
4 |
MV |
1 |
2 |
3 |
4 |
MV |
|
100 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Absolute Numbers of immobileDaphnids after 24 and 48 hours ofExposure in the Definitive Test
(n = 20, divided into 4 replicates with 5 daphnids each)
Nominal test item concentration [mg/L] |
Number of immobile Daphnids / Total number of Daphnids |
|||||||||
24 hours |
48 hours |
|||||||||
Replicates |
Replicates |
|||||||||
1 |
2 |
3 |
4 |
MV |
1 |
2 |
3 |
4 |
MV |
|
100 |
0 / 5 |
0 / 5 |
0 / 5 |
0 / 5 |
0 / 20 |
0 / 5 |
0 / 5 |
0 / 5 |
0 / 5 |
0 / 20 |
Control |
0 / 5 |
0 / 5 |
0 / 5 |
0 / 5 |
0 / 20 |
0 / 5 |
0 / 5 |
0 / 5 |
0 / 5 |
0 / 20 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the limit concentration 100 mg/L of the test item 4-Methoxy-N-methyl-3-nitrobenzamide, no effects on Daphnia magna were observed.
- Executive summary:
In the acute immobilization test with Daphnia magna (STRAUS), the effects of the limit concentration of 100 mg/L of the test item 4-Methoxy-N-methyl-3-nitrobenzamide were determined at the test facility according to OECD 202 (2004) .
The limit test was conducted under static conditions over a period of 48 hours.The limit concentration was visually clear throughout the exposure period. Twenty daphnids, divided into 4 replicates with 5 daphnids each, were exposed to the limit concentration and the control.
The concentration of the test item 4-Methoxy-N-methyl-3-nitrobenzamide was analytically verified via HPLC-DAD in fresh media at the start of the exposure (0 hours) and in old media at the end of the exposure (48 hours) in the limit concentration and in the control.
The measured concentration in the limit concentration was 100% of the nominal concentration in fresh medium at the start of the exposure (0 hours) and 99% of the nominal concentration in old medium at the end of the exposure (48 hours).
The measured test item concentrations were within ± 20% of the nominal concentration. This indicates that the test item concentration was successfully maintained for the duration of the test. Therefore, the effect concentrations given are based on the nominal concentration of the test item4-Methoxy-N-methyl-3-nitrobenzamide.
The validity criteria of the test guideline were fulfilled.
EC10 / 50 / 100-Values (based on the nominal concentration of the test item)
Effect
Concentrations
Test
duration
[h]
4-Methoxy-N-methyl-3-nitrobenzamide
Nominal test item concentration
[mg/L]
EC10 / 50 / 100
24
> 100
48
> 100
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