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EC number: 231-995-1 | CAS number: 7783-40-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 31 January 2017- 3 February 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- resp. EU C.2
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Materion / lot 662599-1
- Expiration date of the lot/batch: 31-Jul 2017
- Purity test date: 18- Jan-2016
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: RT, away from moisture
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: low solubility
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not expected - Analytical monitoring:
- yes
- Details on sampling:
- Samples were WAFs. This was done by weighing the nominal load 100 mg/L (real load 100.1 mg/L), adding tap water and stirring for 24 hours. The resulting solution was filtrated through 0.45 µm Nylon filters. Sampling intervals were 0, 24h and 48h.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
20 hours before the start of the test, the adult animals were separated from the young. 0.5 hours before test start, the adults were caught with the help of a glass tube, and the new-born Daphnia, aged between 0 and 19 hours, were sieved from the medium and immedi-ately placed into a 250 mL-beaker containing dilution water. After a settling-in period of 30 minutes, animals which showed no apparent damage were used for the test.
Switching from M4-medium (husbandry) to Dilution water (test) has been shown not to cause any detrimental effects for test Daphnia. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea, Daphnia magna
- Strain/clone: Berlin
- Source: Umweltbundesamt Berlin
- Age of parental stock: between 0 and 24 hours
- Feeding during test: No feeding during the tests.
- Food type: unicellular green algae (Desmodesmus subspicatus)
ACCLIMATION
- Acclimation period: Daphnia magna is bred in the LAUS GmbH throughout the year. The animals are kept for the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identi-cal. The husbandry is performed similar to the method described in the OECD guideline, following SOP 115 002 01 („Zucht und Hälterung von Daphnia magna STRAUS“)
- Medium: M4-Medium (recipe of ELENDT)
- Type and amount of food: unicellular green algae (Desmodesmus subspicatus)
- Vessels: preserving glasses, nominal volume 2 L
- Health during acclimation: mortality was not observed
- Test type:
- static
- Water media type:
- freshwater
- Remarks:
- Deionised water with an enrichment of certain minerals (dilution water as demanded in the guidelines)
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 19.0 – 20.1 °C
- pH:
- 7.7-7-8
- Dissolved oxygen:
- 8.8-9.5
- Nominal and measured concentrations:
- blank control and nominal concenration of 100 mg/L (real load 100.1 mg/L)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Beakers
- Type: Closed
- Material, size, headspace, fill volume: glass, nominal volume 50 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 vessels, each containing 20 mL test solution and 5 Daphnia
- No. of vessels per control (replicates): 4 vessels, each containing 20 mL test solution and 5 Daphnia
TEST MEDIUM / WATER PARAMETERS
-CaCl2*2H2O: 293.80 mg/L
-MgSO4*7H2O: 123.30 mg/L
-NaHCO3:64.80 mg/L
-KCl: 5.80 mg/L
-Resulting hardness in mmol/L:2.502 mg/L
-Resulting hardness in mg CaCO3/L: 250 mg/L
- Dissolved oxygen and pH: Dissolved oxygen and pH were measured in the batch solutions prepared for each control and treatment group at the beginning of the test and at the 24-hour, and in each test chamber at the end of each 24-hour period. Dissolved oxygen was measured using a Thermo Model 340i wtw dissolved oxygen meter, and measurements of pH were made using a pH meter 340i wtw.
OTHER TEST CONDITIONS
- Photoperiod: 16 hours of light and 8 hours of darkness, using neon tubes
RANGE-FINDING STUDY
- Test concentrations: Blank control and 100 mg/l (Limit test)
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mortality
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- mortality
- Details on results:
- - Mortality of control: none of the Daphnia were immobilised
- Other adverse effects control: Switching from M4-medium (husbandry) to Dilution water (test) has been shown not to cause any detrimental effects for test Daphnia
See section "Any other information on results incl. tables" for details on results (table 4, table 5, and table 6) - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9)
- EC50: 1.7 mg/L (range of 0.6 – 2.1 mg/L) - Validity criteria fulfilled:
- yes
- Remarks:
- -Immobilisation in the blank controls was 0 %. -The lowest concentration of dissolved oxygen at the end of the test was 8.8 mg/L.- There was no variation of pH during the test.
- Conclusions:
- The 48-hour toxicity of water flea (Daphnia magna) to Magnesium Fluoride was determined according to OECD 202 guideline in compliance with GLP. The study was conducted as a limit test and it was based on nominal concentration of 100 mg/l. As a result, EC 50 was determined.
- Executive summary:
The 48-h acute toxicity to water flea (Daphnia magna) was determined by using a static test design. The test followed EU-Method C.2. resp. OECD Guideline 202 and it was perfomed as a limit test using the nominal concentration 100 mg/L.None of the animals were immobilised in the treatment and in the blank control.
At the start and at the end of the test, the content of Magnesium in the test solutions was determined using ICP-OES. Only in the test solution at the end of the test a very low concentration of Magnesium was detectable. All other measured values are below the LOD. Therefore, the determination of the biological results was based on the nominal concentration. Potassium dichromate K2Cr2O7(CAS No. 7778-50-9) was used as positive control to assure that the test conditions are reliable.
No observations were made which might cause doubts concerning the validity of the study outcome. All validity criteria were met.
Reference
Table 4. Immobility | ||||||||||
Nominal Concentration in mg/L | Immobility 24 hours | Immobility 48 hours | ||||||||
absolute | in % | absolute | in % | |||||||
Blank control | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Table 5. pH and O2-values | ||||
Nominal Concentration in mg/L | pH | O2-Concentration in mg/L | ||
0 h | 48 h | 0 h | 48 h | |
Blank control | 7.8 | 7.7 | 9.5 | 8.9 |
100 | 7.8 | 7.8 | 9.5 | 8.8 |
Table 6. Biological Results Test Item | |
Parameter | Value |
24h EC50 | > 100 mg/L |
48h EC50 | > 100 mg/L |
48h NOEC | ≥ 100 mg/L |
48h LOEC | > 100 mg/L |
Description of key information
The acute test (OECD 202) of water flea (Daphnia magna) to Magnesium fluoride was determined using static test system and it was performed as a limit test using a nominal concentration of 100 mg/l. . The study was conducted according to OECD 202 and followed OECD Principles of Good Laboratory Practice (GLP). No unusual observations or deviations from standard test protocol were noted.
As a result, 48-h EC50 was calculated as >100 mg/L.
Based on the available data, the 48-h EC50-value was selected as a key value.
The data obtained from testing on freshwater species has been used as basis for assessment of effects in marine environment as well as for extrapolation of the measured effects to other compartments within the aquatic ecosystem (e.g. sediment) and soil.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Marine water invertebrates
Marine water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
The short-term toxicity to aquatic invertebrates results obtained from the supporting read-across substance (sodium fluoride) has been provided.
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