Propanoic acid, 2-(4-chloro-2-methylphenoxy)-, 2-ethylhexyl ester, (2R)-

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: OECD 202, Part II

Deviations:

no

GLP compliance:

not specified

Specific details on test material used for the study:

MCPP-DMA
Purity: 91.6 %

Analytical monitoring:

not specified

Vehicle:

no

Details on test solutions:

The test solution was prepared by dissolving MCPP-P acid in a dimethylamine (DMA) solution. On the day of the experiment, the MCPP-P DMA salt was diluted into the test medium. MCPP-P DMA was used in the nominal concentrations [calculated as amount of MCPP-P acid]: 2.5, 7.4, 22.2, 66.7 and 200 mg/L.

Test organisms (species):

Daphnia magna

Details on test organisms:

The daphnids were fed with the algae Scenedesmus sp.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Hardness:

no data

Test temperature:

20 °C

pH:

8.0

Dissolved oxygen:

no data

Salinity:

NA

Conductivity:

no data

Nominal and measured concentrations:

Nominal concentrations: 2.5, 7.4, 22.2, 66.7 and 200 mg/L

Details on test conditions:

The study was performed in glass beakers each containing 200 mL reconstituted water. Ten daphnia, less than 24 hours old were exposed for 4 days in duplicate. The daphnids were then separated each in 80 mL beakers and exposed for 21 days at 20 °C and 16 hours light/day (500 -2000 Lux). The test medium was renewed 3 times per week.

Reference substance (positive control):

no

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

22.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

A dose-related increase in mortality of adult daphnids was observed for test concentrations 66.7 and 200 mg/L. After 48 hours, no mortality was observed at any concentration. After 96 hours 25 % were dead at 66.7 mg/L and 25 % at 200 mg/L using 2x10 daphnids. The separation in 10 single survivors into brooding beakers (10x1), revealed 20 % mortality at 66.7 mg/L and 100 % at 200 mg/L after 16 days. The reproduction started after 12 days of exposure.

The study was performed with MCPP-P DMA but the result was caculated as amount of MCPP-P acid.

Validity criteria fulfilled:

yes

Conclusions:

For MCPP-P acid, the hydrolysis product of MCPP-P 2-EHE, a 21d-NOEC of 22.2 mg/L was determined for toxicity to reproduction on Daphnia magna.

Executive summary:

In a semi-static reproduction toxicity test, according to OECD Guideline 202 Part II, for MCPP-P acid a 21d-NOEC of 22.2 mg/L was obseved.

Reference 2

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to section 13 for "Read Across justification".

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

22.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Description of key information

For MCPP-P acid, the hydrolysis product of MCPP-P 2-EHE, a 21d-NOEC of 22.2 mg/L was determined for toxicity to reproduction on Daphnia magna.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

22.2 mg/L

Additional information

The long-term toxicity to aquatic invertebrates was investigated with MCPP-P IOE due to structural similarities (read-across).

In a semi-static reproduction toxicity test, according to OECD Guideline 202 Part II, for MCPP-P acid a 21d-NOEC of 22.2 mg/L was obseved.