1,4-dichloro-2-(1,1,2,3,3,3-hexafluoropropoxy)-5-nitrobenzene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1,4-dichloro-2-(1,1,2,3,3,3-hexafluoropropoxy)-5-nitrobenzene

EC Number:

620-459-6

Cas Number:

130841-23-5

Molecular formula:

C9H3Cl2F6NO3

IUPAC Name:

1,4-dichloro-2-(1,1,2,3,3,3-hexafluoropropoxy)-5-nitrobenzene

Details on test material:

- Name of test material: CA 946 A
- Batch No.: P.906005
- Purity: 96,9%
- Received: 1993-12-15
- Storage: room temperatur
- Stability: 07/96

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples (about 150 ml in duplicate) of each test concentration were drawn from the approximate center of the test vessels. They were takeno immediately before the exposure and after 96 hours exposure and kept at -18 to -22 °C until analysis (see Attachment Table 4)

Test solutions

Vehicle:

yes

Details on test solutions:

- Vehicle: 70,0 mg dimethylformamide (DFM) and 2,9 mg polyoxy-ethylene-sorbitan-monooleate (TWEEN80)/1
- Stock solution: 54,3 mg test substance were added to a mixture of 3,2 g of dimethylformamide (DFM) and 0,1 mg polyoxy-ethylene-sorbitan-monooleate (TWEEN80)/1

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow trout
- Source: P. Hohler / CH-4314 Zeiningen
- Length at study initiation (mean): 51 mm (45-57 mm) based on 7 fishes
- Weight at study initiation (mean): 1,07 g (0,61-1,42 g) based on 7 fishes
- Loading: 0,50 g/l
- Feeding: none
- Adaption: 24 hours / no food 24 hours prior to exposure
- Acclimatization: 26 days
- Treatment: None

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

190 mg CaCo3/1

Test temperature:

14 +/- 1 °C

Nominal and measured concentrations:

Nominal: 0,052 / 0,114 / 0,25 / 0,55 and 1,2 mg test substance/l
Actual end: < 0,004 / < 0,016 / < 0,019 / 0,027 and 0,58 mg test substance/l

Details on test conditions:

TEST SYSTEM
- Vessels: Glass aquaria of 20 liters filled with 15 liters (36/22/25 cm)
- Aeration: Gentle aeration during exposure
- Water: Dechlorinated tap water (carbon filter)
- Lightning: Fluorescent light, 16 hours daily

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.13 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL