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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1996
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report date:
1996

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
3-hydroxy-3'-nitro-2-naphthanilide
EC Number:
205-209-2
EC Name:
3-hydroxy-3'-nitro-2-naphthanilide
Cas Number:
135-65-9
Molecular formula:
C17H12N2O4
IUPAC Name:
3-hydroxy-N-(3-nitrophenyl)-2-naphthamide
Test material form:
solid
Details on test material:
purity: 99.7%
Product number HKAG 155

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Source: waste water treatment plant of Frankfurt am Main
pretreatment: washed, centrifuged and resuspended
aerated before use

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h
Post exposure observation period:
no

Test conditions

Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50 for the toxicity of the substance against bacteria was determined to be greater than 1000 mg/L
Executive summary:

The substance was tested for toxicity to bacteria according to OECD guideline 209. Not activated slude was used from the communale waste water treatment plant of Frankfurt am Main. A limit test with a concentration of 1000 mg/L was performed in duplicate. No effect was determined