Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Justification for type of information:

Data is from GSBL

Qualifier:

according to guideline

Guideline:

other: refer below principle

Principles of method if other than guideline:

Short term toxicity was studied in Pseudomonas fluorescens by BringmannKühnTest.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Pseudomonas fluorescens

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

24 h

Reference substance (positive control):

not specified

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

5 000 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: Mortality

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

5 000 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: swimming behavior

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

5 000 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

other: Cell proliferation inhibition

Validity criteria fulfilled:

not specified

Conclusions:

In toxicity to micro organism the effective concentration i.e EC0 value for test substance Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate (2706-28-7) was found to be 5000 mg/l on Pseudomonas fluorescens by the method BringmannKühnTest for 24 hrs.

Executive summary:

In Toxicity to microorganism study conducted according to Bringmann Kühn Test method on test species Pseudomonas fluorescens for 24 hrs.

During experiment the effective concentration i.e EC0 was found to be 5000 mg/l on the basis of mortality,swimming behavior and Cell proliferation inhibition effects.

Thus based on above mentioned effective concentration the test substance Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate (2706-28-7) consider to be non toxic to micro organisms.

Description of key information

In toxicity to micro organism the effective concentration i.e EC0 value for test substance  Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzenesulphonate (2706-28-7) was found to be 5000 mg/l on Pseudomonas fluorescens by the method BringmannKühnTest for 24 hrs.

Key value for chemical safety assessment

EC50 for microorganisms:

5 000 mg/L

Additional information

Toxicity to micro organism was assessed for the target compound Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzene sulphonate (2706-28-7) and its related read- across. The summary of the results are presented below:

In Toxicity to microorganism study for test substance Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzene sulphonate conducted according to Bringmann Kühn Test method on test species Pseudomonas fluorescens for 24 hrs.

During experiment the effective concentration i.e EC0 was found to be 5000 mg/l on the basis of mortality, swimming behaviour and Cell proliferation inhibition effects.

Whereas experimental study (Chemophere. Vol. 28, No. 12, pp. 2203.2236, 1994) for read across substance 2,2'-(1,2-Ethenediyl)bis(5-aminobenzenesulfonic acid) disodium salt (Cas no. 7336-20-1)indicate that the effect concentrations i.e EC0 of test substance for bacteria (unadapted biological waste water treatment plant) was found to be 1000 mg/l for 24 hrs.

And other experimental study report from HPVIS database for same read across substance i.e 2,2'-(1,2-Ethenediyl)bis(5-aminobenzenesulfonic acid) disodium salt (Cas no. 7336-20-1) indicate that the EC0 was found to be >300 mg/l and EC50 is >100 mg/l for 3 hrs.on Bacteria (activated sludge) and on the basis of growth inhibition effect. For 24 hrs on Pseudomonas fluorescens species effect concentration (EC0)was resulted as 1000 mg/l and Photobacterium phosphoreum species for 45 min study the EC50 was found to be >500 mg/l.

Based on above available studies it is concluded that the substance Disodium 2-amino-5-[(4-sulphonatophenyl)azo]benzene sulphonate (2706-28-7)was consider to be non toxic to micro organisms.