Silver cyanide

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP compliant, guideline study, available as an unpublished report.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2014

Report date:

2014

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Silver cyanide

EC Number:

208-048-6

EC Name:

Silver cyanide

Cas Number:

506-64-9

Molecular formula:

CAgN

IUPAC Name:

silver(1+) cyanide

Test material form:

not specified

Details on test material:

- Name of test material: silver cyanide
- Substance type: not reported
- Physical state: not reported
- Expiration date of the lot/batch: not supplied
- Stability under test conditions: not reported
- Storage condition of test material: Room temperature in the dark.

Test animals / tissue source

Species:

rabbit

Strain:

New Zealand White

Details on test animals or tissues and environmental conditions:

TEST ANIMALS
- Source: Harlan Laboratories UK Ltd., Leicestershire, UK
- Age at study initiation: 12- 14 weeks old
- Weight at study initiation: 2.54 kg
- Housing: in a suspended cage
- Diet: 2930C Tekland Global Rabbit Diet (Harlan Laboratories UK Ltd., Oxon, UK) ad libitum
- Water: drinking water avaialble ad libitum
- Acclimation period: at least 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 17 to 23
- Humidity (%): 30 to 70
- Air changes (per hr): at least 15 air changes per hour
- Photoperiod: 16 h darkness : 8 h light

Test system

Vehicle:

unchanged (no vehicle)

Controls:

yes, concurrent no treatment

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied: 100 mg

Duration of treatment / exposure:

The test item was placed into the conjunctival sac of the right eye, formed by gently pulling the lower lid away from the eyeball. The upper and lower eyelids were held together for about one second immediately after treatment, to prevent loss of the test item, and then released.

Observation period (in vivo):

Assessment of ocular damage or irritation was made approximately 1 and 2 hours after treatment.

Number of animals or in vitro replicates:

The test item was applied to the right eye of one rabbit.

Details on study design:

REMOVAL OF TEST SUBSTANCE
- Washing: none

SCORING SYSTEM: Assessment of ocular damage/irritation was made according to the numerical evaluation (Draize 1977).

TOOL USED TO ASSESS SCORE: Standard ophthalmoscope.

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

overall irritation score

Basis:

animal #1

Time point:

other: 1 hour

Score:

21

Max. score:

110

Results 2

Irritation parameter:

overall irritation score

Basis:

animal #1

Time point:

other: 2 hours

Score:

41

Max. score:

110

Results 3

Irritation parameter:

cornea opacity score

Basis:

animal #1

Time point:

other: 1 hour

Score:

0

Max. score:

80

Results 4

Irritation parameter:

cornea opacity score

Basis:

animal #1

Time point:

other: 2 hours

Score:

20

Max. score:

80

Results 5

Irritation parameter:

iris score

Basis:

animal #1

Time point:

other: 1 hour

Score:

5

Max. score:

10

Results 6

Irritation parameter:

iris score

Basis:

animal #1

Time point:

other: 2 hours

Score:

5

Max. score:

10

Results 7

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

other: 1 hour

Score:

16

Max. score:

20

Results 8

Irritation parameter:

conjunctivae score

Basis:

animal #1

Time point:

other: 2 hours

Score:

16

Max. score:

20

Irritant / corrosive response data:

Iridial inflammation and severe conjunctival irritation were noted 1 hour after treatment. Diffuse corneal opacity, iridial inflammation, severe conjunctival irritation were noted 2 hours after treatment.

Other effects:

Blood stained discharge was noted and the animal appeared lethargic 2 hours after treatment.

Applicant's summary and conclusion

Interpretation of results:

highly irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Silver cyanide was considered to be at least a severe irritant to the rabbit eye and would be classified in category 1 (serious eye damage/irreversible effects on the eye) according to GHS and EU CLP 1272/2008.

Executive summary:

The study was performed to assess the irritancy potential of silver cyanide to the eye of the New Zealand White rabbit. The test was conducted according to OECD 405 and EC B.5 guidelines. A single application of silver cyanide to the non-irrigated eye of one rabbit produced diffuse corneal opacity, iridial inflammation, severe conjunctival irritation and blood stained discharge. The animal was also lethargic two hours after dosing. The test item, silver cyanide, was considered to be at least severely irritant to the rabbit eye (based on one rabbit only) and, therefore, would be classified in category 1 (serious eye damage/irreversible effects on the eye) according to GHS and EU CLP 1272/2008.