Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
Reaction mass of sodium amino-bis{[4-(ethenylsubstituted)phenyl]diazenyl}-hydroxynaphthalenesulfonate and polysodium amino-{[4-(ethenylsubstituted)phenyl]diazenyl}-{[4-(ethenylsubstituted)-2-sulfonatophenyl]diazenyl}-hydroxynaphthalenesulfonate and polysodium amino-bis{[4-(ethenylsubstituted)-2-sulfonatophenyl]diazenyl}-hydroxynaphthalenesulfonate
EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Guidelines followed
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
None - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Activated sludge from a biologic sewage treatment plant was used. The chosen plant treats mostly domestic sewage.
The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf.
Date of collection: 24. Jul. 2014, batch no: 20140724.
The sludge was filtrated through a clean cotton cloth, then washed with tap water twice, with test medium once and re-suspended in test medium. It was then aerated until use in the test. Determination of dry matter gave 4080 mg suspended solids/litre. - Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- ca. 103.8 mg/L
- Based on:
- other: Test item
- Initial conc.:
- ca. 40.8 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- The following instruments and devices were used in the performance of the study:
- Data logger for temperature, ebro
- Analytical scales Mettler Toledo XS DU 205
- Precision scales Mettler Toledo XS 6001S
- Adjustable pipettes with one-way tips Rainin®;
- Carbon analyser TOC multi N/C 2100S, Analytik Jena
- Glass measuring flasks
- pH-meter 3310 wtw
- Heating chamber Heraeus
- Orbital shaker GFL 3017
- Ultrasonic bath Sonorex RK 510H Bandelin
- Fridge - Reference substance:
- aniline
- Preliminary study:
- None
- Test performance:
- None
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 51
- Sampling time:
- 14 d
- Remarks on result:
- other: The total biodegradation in the toxicity control after 14 days was 51 % (criterion: > 35 %).
- Details on results:
- None
- Results with reference substance:
- None
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not readily biodegradable
- Conclusions:
- The following data were determined for the test item FAT 40865/A TE:
10-day-window: not detected
Degradation at the end of 10-day-window: not detected
Degradation at the end of the test: 0 %
Therefore, the test item FAT 40865/A TE is not readily biodegradable following OECD test guideline No. 301A/EU method C.4-A.
The test item can be stated as “not toxic to the inoculum at a concentration of 103.6 mg/L.” - Executive summary:
The test item FAT 40865/A was investigated for its ready biodegradability over 28 days following DOC die-away test according to OECD Test Guideline for the Testing of Chemicals No. 301A and EU Method No. C.4-A.
Activated sludge was used as inoculum at a concentration of 25 mg dry matter/L.
Aniline was used as positive control and at the end of 4 days, 98 % degradation was recorded.
In the toxicity control, the biodegradation amounted to 51 % within 14 days. Therefore, according to the test guideline, the test item can be stated as “not toxic to the inoculum at a concentration of 103.6 mg/L.”
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Guideline:
- OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.9 (Biodegradation: Zahn-Wellens Test)
- Deviations:
- yes
- Remarks:
- The composition of the medium is taken from OECD Guideline. As in EU-Method C.9 a different composition is given, this is stated as a deviation from the EU Method.
- Principles of method if other than guideline:
- None
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
None - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Activated sludge from a biologic sewage treatment plant was used. The chosen plant is treating mostly household sewage. The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf.
Date of collection: 07. Aug. 2014
The sludge was washed with tap water twice, then filtrated through a cloth, resuspended in test medium and then aerated.
The dry matter was determined as 4100 mg suspended solids/L. - Duration of test (contact time):
- ca. 28 d
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- None
- Reference substance:
- aniline
- Preliminary study:
- None
- Test performance:
- None
- Parameter:
- % degradation (DOC removal)
- Value:
- 8
- Sampling time:
- 28 d
- Details on results:
- None
- Results with reference substance:
- None
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- The test item can be considered as “not inherently biodegradable“.
The degree of biodegradation reached 8 % after 28 days. - Executive summary:
The Inherent biodegradability of FAT 40865/A was determined in a 28 day Zahn-Wellens test following OECD Test Guideline for the Testing of Chemicals No. 302B and EU Method No. C.9 at a nominal concentration of 200 mg organic carbon/L in test medium Aniline was used as positive control, and at the end of 5 days, 99 % degradation was recorded. Activated sludge from a domestic sewage treatment plant was used as inoculum (concentration 601 mg dry matter/L). To measure the degradation of test item and positive control, the content of dissolved organic carbon (DOC) in the test vessels was measured seven times during the test. All validity criteria were met. The per cent biodegradation of the test item was calculated based on dissolved organic carbon (DOC).
The following data could be determined for the test item FAT 40865/A TE:
Degradation at the end of the test: 8%
Lag-phase: Day 0 - 16
Degradation phase: Not determinable
Therefore, FAT 40865/A TE is stated as not inherently biodegradable.
Referenceopen allclose all
Findings and results
DOC-Values
DOC Values in mg/L before addition of the test medium to an end volume of 250 mL
Day |
Procedure Control 1 |
Procedure Control 2 |
Positive Control 1 |
Positive Control 2 |
Test Item |
||||
Replicate 1 |
Replicate 2 |
Abiotic Control |
Adsorption Control |
Toxicity Control |
|||||
0 |
0.79 |
1.16 |
67.48 |
77.03 |
37.1 |
40.1 |
47.66 |
48.51 |
103.12 |
Volume in mL |
100 |
100 |
119 |
119 |
125 |
125 |
128 |
128 |
145 |
theoretical value |
0.32 |
0.46 |
32.25 |
36.81 |
18.62 |
20.12 |
24.39 |
24.83 |
59.78 |
measured value |
0.00 |
0.00 |
40.05 |
40.05 |
40.76 |
40.76 |
40.77 |
40.85 |
80.58 |
DOC Values in the test in mg/L
Day |
Procedure Control 1 |
Procedure Control 2 |
PositiveControl 1 |
PositiveControl 2 |
Test Item |
|||||||
Replicate 1 |
Replicate 2 |
Abiotic Control |
Adsorption Control |
Toxicity Control |
||||||||
0.125 |
1.86 |
1.45 |
42.07 |
42.68 |
22.47 |
24.10 |
26.93 |
27.72 |
59.52 |
|||
4 |
0.90 |
0.73 |
1.35 |
1.62 |
24.67 |
24.13 |
30.19 |
29.79 |
24.17 |
|||
11 |
1.27 |
1.11 |
1.20 |
1.70 |
24.15 |
24.54 |
29.57 |
30.75 |
29.16 |
|||
18 |
0.22 |
0.11 |
1.55 |
0.67 |
25.95 |
27.93 |
29.81 |
28.65 |
28.97 |
|||
28 |
1.66 |
1.22 |
2.56 |
1.24 |
27.22 |
30.05 |
32.74 |
34.80 |
31.39 |
|||
The calculated values for the percentage degradation are presented in the following table (Degradation values in %):
Day |
Positive Control 1 |
Positive Control 2 |
Test item |
||||
Replicate 1 |
Replicate 2 |
Abiotic Control |
Adsorption Control |
Toxicity Control |
|||
4 |
98.7 |
98.0 |
-14.6 |
-3.9 |
-12.1 |
-11.2 |
59.6 |
11 |
100.0 |
98.8 |
-10.3 |
-4.0 |
-9.8 |
-13.4 |
51.7 |
18 |
96.6 |
98.8 |
-23.9 |
-23.7 |
-10.7 |
-9.3 |
50.2 |
28 |
97.2 |
100.5 |
-23.9 |
-27.5 |
-21.6 |
-28.0 |
48.2 |
The measured DOC concentrations at the end of the test in the test replicates and in the abiotic control were slightly higher than at the beginning. This might be caused by dissolution of undissolved test item during the test. Therefore, negative degradation values were calculated.
Validity
- The difference within replicates at the end of the test was 3.6 % (criterion: <= 20%).
- The positive control reached the pass level of 98 % on day 4 (criterion: > 70% within 14 days).
- The total biodegradation in the toxicity control after 14 days was 51 % (criterion: > 35 %).
All the criteria were met to validate this study according to test guideline.
DOC Values
The measured/recalculated DOC-values in mg/L at the beginning (before addition of the inoculum):
Day |
Control |
Positive |
Test Item |
Abiotic Control |
Toxicity Control |
|||
1 |
2 |
1 |
2 |
1 |
2 |
|||
Measured Value |
1.21 |
0.87 |
286.35 |
283.51 |
226.18 |
222.65 |
221.61 |
503.54 |
Calculated Start Conc. |
0.81 |
0.58 |
190.77 |
188.88 |
150.79 |
148.43 |
147.79 |
335.81 |
Theoretical Start Conc. |
0.00 |
0.00 |
200.00 |
200.00 |
200.27 |
200.27 |
200.13 |
399.73 |
Calculated start concentrations are based on DOC measurement of the test solutions with a volume of 1 L (before adding inoculum and filling up to the final volume of 1500 mL). Theoretical start concentrations are based on the organic carbon content of test item resp. positive control, based on TOC measurement of the respective stock solutions.
The measured DOC values (mg/L) in the course of the test are presented in the following table:
Day |
Control |
Positive control |
Test Item |
Abiotic |
Toxicity |
|||
1 |
2 |
1 |
2 |
1 |
2 |
|||
0.125 |
2.04 |
1.10 |
194.19 |
196.69 |
171.45 |
166.95 |
167.21 |
360.27 |
5 |
4.76 |
4.74 |
6.88 |
6.31 |
172.70 |
185.98 |
182.90 |
170.60 |
12 |
4.46 |
4.87 |
4.55 |
4.03 |
183.34 |
172.94 |
188.27 |
187.27 |
19 |
6.24 |
5.93 |
7.00 |
5.84 |
137.14 |
140.80 |
187.39 |
180.26 |
27 |
5.84 |
3.59 |
4.25 |
5.91 |
147.93 |
132.69 |
192.38 |
118.70 |
28 |
4.32 |
3.66 |
5.75 |
3.72 |
161.67 |
155.20 |
186.63 |
152.32 |
29* |
5.19 |
4.14 |
5.84 |
6.74 |
121.05 |
130.07 |
148.89 |
108.25 |
*repeated measurement on day 29
Calculation of biodegradation was based only on the content of dissolved organic carbon. Because of degradation in conjunction with precipitation and dissolution of test item during the running study, scattering DOC values and scattering degradation values were likely to occur.
These effects are confirmed by the measured DOC concentrations in the test replicates and the toxicity control on day 28. The DOC concentrations were much higher than on day 27. For verification, an additional measurement on day 29 was performed. The measured values on Day 29 were much lower than the previously measured values. The measured DOC values in the abiotic control were also significantly lower on day 29 compared to day 28.
pH Values
pH was measured at each sampling time or once per week and, if necessary, adjusted to pH 7.0 ± 0.5. The values are presented in the following table:
Day |
Control |
Positive Control |
Test Item |
Abiotic Control |
Toxicity Control |
|||
1 |
2 |
1 |
2 |
1 |
2 |
|||
0 |
7.6 to 7.3 |
7.6 to 7.4 |
7.7 to 7.3 |
7.7 to 7.2 |
7.6 to 7.4 |
7.6 to 7.3 |
7.1 |
7.6 to 7.2 |
5 |
6.9 |
6.9 |
7.8 to 7.0 |
7.8 to 7.1 |
7.2 |
7.2 |
7.2 |
7.8 to 7.1 |
12 |
6.7 |
6.8 |
7.1 |
7.1 |
7.2 |
7.2 |
7.1 |
7.3 |
19 |
6.6 |
6.7 |
5.1 to7.2 |
5.2 to 6.9 |
7.2 |
7.2 |
7.1 |
6.9 |
27 |
6.4 to 7.3 |
6.6 |
6.3 to 6.6 |
6.0 to 6.8 |
7.1 |
7.1 |
7.2 |
6.0 to 6.7 |
Description of key information
FAT 40865/A TE is stated as not inherently biodegradable.
Key value for chemical safety assessment
Additional information
The test item FAT 40865/A was investigated for its ready biodegradability over 28 days following DOC die-away test according to OECD Test Guideline for the Testing of Chemicals No. 301A and EU Method No. C.4-A. Activated sludge was used as inoculum at a concentration of 25 mg dry matter/L. Aniline was used as positive control and at the end of 4 days, 98 % degradation was recorded. In the toxicity control, the biodegradation amounted to 51 % within 14 days. Therefore, according to the test guideline, the test item can be stated as “not toxic to the inoculum at a concentration of 103.6 mg/L.
In another study, the Inherent biodegradability of FAT 40865/A was determined in a 28 day Zahn-Wellens test following OECD Test Guideline for the Testing of Chemicals No. 302B and EU Method No. C.9 at a nominal concentration of 200 mg organic carbon/L in test medium Aniline was used as positive control, and at the end of 5 days, 99 % degradation was recorded. Activated sludge from a domestic sewage treatment plant was used as inoculum (concentration 601 mg dry matter/L). To measure the degradation of test item and positive control, the content of dissolved organic carbon (DOC) in the test vessels was measured seven times during the test. All validity criteria were met. The percent biodegradation of the test item was calculated based on dissolved organic carbon (DOC).
The following data could be determined for the test item FAT 40865/A TE:
Degradation at the end of the test: 8%
Lag-phase: Day 0 - 16
Degradation phase: Not determinable
Therefore, FAT 40865/A TE is stated as not inherently biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.