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EC number: 418-570-8 | CAS number: 25383-07-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- June 23, 1998 - June 24, 1998
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
Test material (Fosfomycin PEA salt): 100 ml of an aqueous Fosfomycin PEA salt stock solution at 500 mg/l were prepared and transferred to the suitable 500 ml beaker and kept gently stirred unter the start of the contact time. The concentration of use was 100 mg/l.
Reference substance (3,5-dichlorophenol): A stock solution at 500 mg/l was prepared. 0.05 g of standard were dissolved in 1 ml 1N NaOH, diluted to about 3 ml with bidistilled water. Excess NaOH was neutralized with 1N H2SO4 to the point of incipient precipitation. Thereafter the mixture was made up to 0.1 litres.
- Controls: Yes. - Test organisms (species):
- other: Activated sludge from the RBM laboratory water treatment plant.
- Details on inoculum:
- - Preparation of inoculum for exposure: 2 L of wet sludge were suspended in water in order to obtain an activated sludge with a liquor/suspended solids level of 4 g/l was calculated. 2 L of wet sludge were suspended in water in order to obtain an activated sludge with a liquor/suspended solids level of 4 g/l. To that mixture 50 ml/l of synthetic sewage feed were added.
- Initial biomass concentration: 4 g/l (dwt) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- After 3 hours, sample's oxygen measurement at 15 minute intervals.
- Test temperature:
- 20 ºC
- pH:
- 6.82-7.00
- Nominal and measured concentrations:
- Nominal concentrations: 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Beakers
- Material, size, headspace, fill volume: Glass, 500 ml
- Aeration: Air-flow using compressed air.
- No. of organisms per vessel: 4 g/l of activated sludge
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: See the table below.
Synthetic sewage feed (1 litre of water):
16 g peptone
11 g meat extract
3 g urea
0.7 g NaCl
0.4 g CaCl2·2H2O
0.2 g MgSO4·7H2O
2.8 g K2HPO4
- Intervals of water quality measurement: Starting pH of each sample.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Oxygen consumption over a period of 10 minutes
TEST CONCENTRATIONS
- Test concentrations: Nominal concentrations: 100 mg/l - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol (1, 3.2, 10, 32 and 50 mg/l)
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Relevant effect levels: EC50 was 10.8 mg/L (95% CL: 10.7-10.9) - Reported statistics and error estimates:
- The EC50 was calculated by the Probit method modified by the Flemish Institue for Technological Research (VITO).
- Validity criteria fulfilled:
- yes
- Remarks:
- Variation between the respiration rates of the 2 control samples < 15%. Inhibitory effect of 3,5-dichlorophenol was within the range of the EC50 value of 5 to 30 mg/l
- Conclusions:
- EC50 value (based on respiration inhibition) on activated sludge at 3 hours exposure period was > 100 mg/l.
- Executive summary:
The assessment of the effect of the test article Fosfomycin PEA salt on microorganisms by measuring the respiration rate followed the OECD Guideline 209. 4 g/l of activated sludge from RMB laboratory sewage plant were exposed for 3 hours of aeration at 20 ºC to test material at the nominal concentration of 100 mg/l. The reference substance, 3,5 -dichlorophenol was tested at 1, 3.2, 10, 32 and 50 mg/l and two control tests were carried out.
At 100 mg/l the test article showed 18.9 % inhibition on the respiration rate. The EC50 can be defined as >100 mg/l. The EC50 of the reference compound under the same conditions was 10.8 mg/l, which is in the accepted guideline range (5 -30 mg/l).
Reference
Table 2. Summary of results.
Beaker |
Chemical |
Concentration |
Oxygen consumption (mgO2/L min) |
Inhibition (%) |
Starting pH |
7 |
Fosfomycin PEA salt |
100 |
0.45 |
18.9 |
6.82 |
1 |
Control |
- |
0.58 |
- |
7.0 |
8 |
Control |
- |
0.53 |
- |
7.0 |
2 |
3,5-dichlorophenol |
50 |
0.03 |
95 |
6.96 |
3 |
3,5-dichlorophenol |
32 |
0.10 |
82 |
6.96 |
4 |
3,5-dichlorophenol |
10 |
0.44 |
20.7 |
6.98 |
5 |
3,5-dichlorophenol |
3.2 |
0.45 |
18.9 |
6.97 |
6 |
3,5-dichlorophenol |
1.0 |
0.46 |
17.1 |
6.99 |
Description of key information
Key study: Test method according to OECD Guideline 209. GLP study.
The EC50 value (based on respiration inhibition) on activated sludge at 3 hours exposure period was > 100 mg/l.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
Key study: Test method according to OECD Guideline 209. GLP study. The EC50 value (based on respiration inhibition) on activated sludge at 3 hours exposure period was > 100 mg/l.
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