Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Repeated dose toxicity: other routes

Currently viewing:

Administrative data

Endpoint:
short-term repeated dose toxicity: other route
Type of information:
experimental study
Adequacy of study:
disregarded due to major methodological deficiencies
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Unsuitable test system

Data source

Reference
Reference Type:
publication
Title:
Long-Term Treatment with 2,5-Hexanedione Has No Effect on the Specific Activity of Some Brain and Liver Glycolytic Enzymes of Adult Rats
Author:
M.E. Pereira, A.M. Bordignon, C. Bürger, C.I. Huang, J.B.T. Rocha
Year:
1991
Bibliographic source:
Brazilian J. Med Biol. Res. 1991, 24, 735-740

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
not specified
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Hexane-2,5-dione
EC Number:
203-738-3
EC Name:
Hexane-2,5-dione
Cas Number:
110-13-4
Molecular formula:
C6H10O2
IUPAC Name:
hexane-2,5-dione
Test material form:
not specified
Details on test material:
no data

Test animals

Species:
rat
Strain:
Wistar
Sex:
female
Details on test animals or test system and environmental conditions:
female Wistar rats, 40-45 days old, 150-170 g bw, 12 h light/12 h dark cycle

Administration / exposure

Route of administration:
subcutaneous
Vehicle:
other: 100 mM NaCl, 10 mM K-Phosphate buffer, pH = 7
Details on exposure:
200 mg/ kg bw 2,5- Hexanedione

Analytical verification of doses or concentrations:
not specified
Duration of treatment / exposure:
40 days
Frequency of treatment:
daily
Doses / concentrations
Remarks:
Doses / Concentrations:
200 mg/ kg bw
No. of animals per sex per dose:
one dose group
Control animals:
yes
Details on study design:
40 days treatment, examination of brain and liver after treatment.
Enzymes: Lactic dehydrogenase, Enolase, Malate dehydrogenase, Liver glycogene level

Examinations

Observations and examinations performed and frequency:
Body weight, food and water intake were measured every 6 days.
Sacrifice and pathology:
Decapitation, removal and analysis of brain and liver.
Statistics:
no data

Results and discussion

Results of examinations

Clinical signs:
no effects observed
Mortality:
no mortality observed
Body weight and weight changes:
effects observed, treatment-related
Description (incidence and severity):
reduced body weight gain
Food consumption and compound intake (if feeding study):
no effects observed
Water consumption and compound intake (if drinking water study):
effects observed, treatment-related
Description (incidence and severity):
increase

Effect levels

Dose descriptor:
other: enzyme activity
Effect level:
ca. 200 mg/kg bw/day
Based on:
test mat.
Sex:
female

Target system / organ toxicity

Critical effects observed:
not specified

Applicant's summary and conclusion

Conclusions:
Reduced weight gain, increased water intake, no changes in enzyme activity in brain and liver.