Registration Dossier
Registration Dossier
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EC number: 812-739-3 | CAS number: 157357-30-7
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
EU Method C.1, OECD Guideline 203, GLP, key study, validity 2:
96h-LC50 = 13 mg/L (95% CI: 7.5-23 mg/L)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 13 mg/L
Additional information
One key study is available to assess the 96h acute toxicity of the registered substance to zebra fish (Brachydanio rerio, now Danio rerio) under semi-static conditions with a daily test medium renewal according to the EU Method C.1 and the OECD Guideline 203, with GLP statement. The recorded effects were mortality and visible abnormalities of the test fish.
The nominal test substance concentrations tested were 4.6, 10, 22, 46 and 100 mg/L. Additionally, a control group was tested in parallel. Since the test substance was determined to be volatile, the test was performed in a closed system to avoid losses of the volatile substance.
In the analyzed test medium samples the measured test substance concentrations ranged between 64 and 122% of the nominal values. The mean measured test substance concentrations (calculated as the average overall measurements per test concentration) varied in the range of 75 to 119% of the nominal values. Therefore, the reported biological results are related to the mean measured test substance concentrations, corresponding to 5.0, 7.5, 23, 55 and 110 mg/L.
All validity criteria were fulfilled but the measured no effect and 100% effect concentrations were adjacent with a difference of more than a factor of three. The 96h-LC50 value was determined as a geometric mean value of the two consecutive test concentrations with 0 and 100% mortality, at 13 mg/L (95% confidence interval: 7.5 -23 mg/L).
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