2-amino-4,6-dinitrophenol

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

publication

Title:

Tetratox: Tetrahymena pyriformis population growth impairment endpoint-a surrogate for fish lethality.

Author:

Schultz, T.W.

Year:

1997

Bibliographic source:

Toxicology methods, 7, 289-309, 1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other:

Principles of method if other than guideline:

A short-term, static protocol using the common freshwater ciliate Tetrahymena pyriformis (strain GL-C) is reported. The 50% impairment growth concentration (IGC 50) is the endpoint of choice. Cultures are reared in 50 mL of a semi defined medium in 250-mL Erlenmeyer flasks. Definitive test replicates consist of a minimum of 5 different concentrations of each test material. Duplicate flasks are inoculated to an initial density of 2500 cells/ mL with log-growth phase ciliates. Following 40 h of incubation at 27°C, population density is measured spectrophotometrically and 50% effect levels are determined

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-amino-4-nitrophenol

EC Number:

202-767-9

EC Name:

2-amino-4-nitrophenol

Cas Number:

99-57-0

IUPAC Name:

2-amino-4-nitrophenol

Details on test material:

- Name of test material: 2-amino-4-nitrophenol
- Substance type:organic
- Physical state:solid

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Tetrahymena pyriformis

Details on inoculum:

Tetrahymena pyriformis (strain GL-C) is used.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Test conditions

Test temperature:

27°C

Details on test conditions:

Cultures are reared in 50 mL of a semi defined medium in 250-mL Erlenmeyer flasks. Definitive test replicates consist of a minimum of 5 different concentrations of each test material. Duplicate flasks are inoculated to an initial density of 2500 cells/ mL with log-growth phase ciliates. Following 40 h of incubation at 27°C, population density is measured spectrophotometrically and 50% effect levels are determined

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

IC50

Effect conc.:

52.2 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Applicant's summary and conclusion

Conclusions:

The inhibitory concentration (IC50) of 2-amino-4-nitrophenol in microorganism (Tetrahymena pyriformis) in a 48hr study on the basis of growth inhibition effect was found to be 52.2 mg/L.

Executive summary:

The inhibitory concentration (IC50) of 2-amino-4-nitrophenol in microorganism (Tetrahymena pyriformis) in a 48hr study on the basis of growth inhibition effect was found to be 52.2 mg/L.