Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 257-399-1 | CAS number: 51765-51-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- data is from peer reviewed journals
Data source
Reference
- Reference Type:
- publication
- Title:
- Investigations into the Immunotoxicity and Allergic Potential Induced by Topical Application of N-Butylbenzenesulfonamide (NBBS) in a Murine Model
- Author:
- Antonella Marrocco, B. Jean Meade, Carrie M. Long, Ewa Lukomska, Nikki B. Marshall, and Stacey E. Anderson
- Year:
- 2 015
- Bibliographic source:
- J Toxicol Environ Health A; 2015; 78(17): 1122–1132
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: method described in the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) Peer Review Panel report .
- Principles of method if other than guideline:
- N-butylbenzenesulphonamide [NBBS] was tested in a combined irritancy/local lymph node assay (LLNA) on Female BALB/c mice.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- N-butylbenzenesulphonamide
- EC Number:
- 222-823-6
- EC Name:
- N-butylbenzenesulphonamide
- Cas Number:
- 3622-84-2
- Molecular formula:
- C10H15NO2S
- IUPAC Name:
- N-butylbenzenesulfonamide
- Test material form:
- liquid: viscous
- Details on test material:
- Name of the test chemical:N-butylbenzenesulphonamide [NBSS]
Molecular Formula:C10H15NO2S
Molecular Weigth:213.299 g/mol
InChI: 1S/C10H15NO2S/c1-2-3-9-11-14(12,13)10-7-5-4-6-8-10/h4-8,11H,2-3,9H2,1H3
Substance Type: Organic
Physical State: Liquid
Constituent 1
- Specific details on test material used for the study:
- Name of the test chemical: N-butylbenzenesulphonamide [NBSS]
Molecular Formula:C10H15NO2S
Molecular Weigth:213.299 g/mol
InChI: 1S/C10H15NO2S/c1-2-3-9-11-14(12,13)10-7-5-4-6-8-10/h4-8,11H,2-3,9H2,1H3
Substance Type: Organic
Physical State: Liquid
Test animals
- Species:
- mouse
- Strain:
- Balb/c
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Sex: Female
- Source: Taconic Farms (Germantown, NY)
- Age:5–9 weeks
- Weight at study initiation: 18-20g
- Fasting period before study: none
- Housing: housed 5 per cage in ventilated plastic shoebox cages with hardwood chip bedding
- Diet (ad libitum): NIH-31 modified 6% irradiated rodent diet (Harlan Teklad), ad libitum
- Water: tap water, ad libitum
- Acclimation period: allowed to acclimate for a minimum of 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): maintained between 18 and 73°C
- Humidity (%): relative humidity between 30 and 70%.
- Air changes (per hr): not mentioned
- Photoperiod (hrs dark / hrs light): The light/dark cycle was maintained on 12-h intervals
All animal experiments were performed in the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC)-accredited National Institute for Occupational Safety and Health animal facility in accordance with an animal protocol approved by the Institutional Animal Care and Use Committee
Test system
- Type of coverage:
- open
- Preparation of test site:
- not specified
- Vehicle:
- other: acetone
- Controls:
- yes
- Amount / concentration applied:
- 25, 50 and 100% (w/v) solution of NBSS - test group
Acetone (25 microliters) - vehicle control
dinitrofluorobenzene (DNFB) - 0.3% - positive control - Duration of treatment / exposure:
- 3 days
- Observation period:
- 3 days
- Number of animals:
- 3/group
- Details on study design:
- TEST SITE
- Area of exposure: ears
SCORING SYSTEM: Mice were observed daily for signs of distress or extreme irritation, for any signs of chemical-induced toxicity (ruffled fur, abnormal posture, isolation in the cage, abnormal exudates from the eyes, nose, or anus), and for local signs of irritation, including ear erythema and swelling.
Results and discussion
In vitro
- Other effects / acceptance of results:
- no data available
In vivo
Results
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- 72 h
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Irritant / corrosive response data:
- Dermal exposure of female BALB/c mice to the high concentration of NBBS (100%) produced no signs of overt toxicity or visual signs of inflammation at the exposure sites. A numerical increase in ear swelling was observed following exposure to NBBS (8.4, 7.2, and 5.6% for 25, 50, and 100% respectively). DNFB (0.3%), used as a positive control for irritancy studies, showed a mean significant rise of 241% ear swelling after exposure
Applicant's summary and conclusion
- Interpretation of results:
- other: not irritating
- Conclusions:
- N-butylbenzenesulphonamide [NBBS] was tested in a combined irritancy/local lymph node assay (LLNA) on Female BALB/c mice.
Dermal exposure of female BALB/c mice to the high concentration of NBBS (100%) produced no signs of overt toxicity or visual signs of inflammation at the exposure sites.
Hence, N-butylbenzenesulphonamide [NBSS] can be considered not irritating to skin of BALB/c mice. - Executive summary:
N-butylbenzenesulphonamide[NBBS] was tested in a combined irritancy/local lymph node assay (LLNA) on Female BALB/c mice,according to the method described in the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) Peer Review Panel report. Mice (3 per group) were exposed topically on each ear to 25 μl vehicle (acetone) or 25,50 or 100% NBSS. Dinitrofluorobenzene [DNFB] (0.3%) was used as a positive control. Animals were allowed to rest for 2 d following the last exposure and then euthanized via CO2 inhalation. Mice were observed daily for signs of distress or extreme irritation, for any signs of chemical-induced toxicity (ruffled fur, abnormal posture, isolation in the cage, abnormal exudates from the eyes, nose, or anus), and for local signs of irritation, including ear erythema and swelling. Animals were weighed the day prior to the first exposure and on d 4 prior to euthanasia.
Dermal exposure of female BALB/c mice to the high concentration of NBBS (100%) produced no signs of overt toxicity or visual signs of inflammation at the exposure sites. A numerical increase in ear swelling was observed following exposure to NBBS (8.4, 7.2, and 5.6% for 25, 50, and 100% respectively). DNFB (0.3%), used as a positive control showed a mean significant rise of 241% ear swelling after exposure.
Hence,N-butylbenzenesulphonamide[NBSS] can be considered not irritating to skin of BALB/c mice.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.