Ammonium 4-acetoacetylamino-5-methoxy-2-methylbenzenesulphonate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well performed OECD study with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Ammonium 4-acetoacetylamino-5-methoxy-2-methylbenzenesulphonate

EC Number:

276-769-3

EC Name:

Ammonium 4-acetoacetylamino-5-methoxy-2-methylbenzenesulphonate

Cas Number:

72705-22-7

Molecular formula:

C12H15NO6S.H3N

IUPAC Name:

ammonium 4-acetoacetylamino-5-methoxy-2-methylbenzenesulphonate

Details on test material:

purity 94.8 % (contains residual water)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

At 0, 48 and 96 hours samples of approximately 100 ml were taken out of the midth of the bassin and analyzed by HPLC (UV-detection)

Test solutions

Vehicle:

no

Details on test solutions:

Substance and dilution water were homogenized with ultrasonic and Ultra-Turrax. The substance was completely dissolved.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrabärbling
- Strain: Hamilton - Buchanan
- Source: West Aquarium, 3422 Bad Lauterberg
- Age at study initiation: approximately 3 - 4 months
- Length at study initiation: 2.6 - 3.3 cm
- Feeding during test: no

ACCLIMATION
- Acclimation period: about 14 days
- Type and amount of food: Tetra Min, Tetra Werke, Melle
- Feeding frequency: twice a day, ad libitum

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

no

Test conditions

Test temperature:

21.0 - 23.0 °C

pH:

7.4 - 8.4

Dissolved oxygen:

7.4 - 8.4 mg/L

Nominal and measured concentrations:

nominal: 500 mg/L
measured: 470.3 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: open glass-vessel (30 cm x 22 cm x 24 cm), calibrated to 10 liters
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Preparation of dilution water: according to ISO/DIS 7346/1

OTHER TEST CONDITIONS
- Photoperiod: 12 hours dark / 12 hours light
- Light intensity: 700 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Test concentrations: 0 mg/L and 500 mg/L (nominal)

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Control of the fish after 2 -4, 24, 48, 72, 96 hours
- Behavioural abnormalities: change in swimming and behaviour , irregular aspirate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The substance has not to be classified for acute toxicity to fish, since LC50 is greater than 500 mg/L (nominal).

Executive summary:

The substance was tested for acute toxicity to fish according to OECD guideline 203. One concentration with nominal 500 mg/L (measured 470.3 mg/L) was used and a control, each with 10 fish per test. After 96 hours none of the Zebrabärbling out of the test group or control group was dead.

The LC is therefore greater than 500 mg/L