4-methoxyphenylacetone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 July - 6 September 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study (OECD 202) and in compliance with GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

TH 1165 I

IUPAC Name:

TH 1165 I

Details on test material:

- Name of test material (as cited in study report): TH 1165 I; (4-Methoxyphenyl)-aceton
- Physical state: yellow liquid
- Analytical purity: 99.01 %
- Purity test date: 6. March 2000
- Lot/batch No.: 14
- Expiration date of the lot/batch: can be requested from the sponsor
- Stability under test conditions: can be requested from the sponsor
- Storage condition of test material: in original container, at room temperature, in the dark

Test solutions

Vehicle:

no

Details on test solutions:

The test medium with the test item concentration of nominal 100 mg/L was prepared by dissolving 50 mg test item homogeneously in 500 ml test water by intense stirring for 15 minutes and short ultrasonic treatment for 2 minutes. The test medium was freshly prepared just before the start of the test (= introduction of Daphnia).

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna (Straus)
- Source: supplied 1997 by the Umweltbundesamt, Institut für Wasser-, Boden- und Lufthygiene, Berlin, Germany
- Age at study initiation (mean and range, SD): 7.5 - 23.5 hours
- Sex: female
- Method of breeding: The Daphnia were bred in the laboratories of IBACON under similar temperature and light conditions as in the test, and in reconstituted water of a similar quality regarding pH, components of the main ions and total hardness as the test water used in the test. The test organisms were not first brood progeny.

ACCLIMATION
- Acclimation period: for 7.5 hours under test conditions
- Acclimation conditions (same as test or not): yes, similar
- Type and amount of food: none

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

Water hardness was reported as 250 mg/L CaCO3

Test temperature:

21°C

pH:

8.0

Dissolved oxygen:

The dissolved oxygen concentration at the end of the test was >60% of the air saturation value at the temperature used.

Salinity:

Not applicalbe

Nominal and measured concentrations:

nominal concentration: 100 mg/l

Details on test conditions:

TEST SYSTEM
The Test vessels were glass beakers of 50 ml test medium. Each concentration had 2 replicate vessels and each vessel contain 10 test organisms. The age of the test organisms were < 24 hours and they were not fed during the test. Vessels were kept at 21.0°C .

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

A 48 hour EC50 value higher than 100 mg test item/L was determined in a reliable study conducted according to an appropriate OECD test guideline.