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EC number: 252-354-2 | CAS number: 35086-59-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 January 1999 - 18 June 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted in GLP compliance and according to an OECD guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3,5-Diacetoxyacetophenon
- IUPAC Name:
- 3,5-Diacetoxyacetophenon
- Test material form:
- other: solid
- Details on test material:
- - Physical state: Brownish solid
- Analytical purity: Not indicated by sponsor; treated as 100% pure
- Lot/batch No.: 286
- Expiration date of the lot/batch: 16 December 1999
- Storage condition of test material: At room temperature in the dark
- Other:
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- During the final test samples were taken from 10, 32 and 100 mg/1 for analysis. Also samples
were taken from the controls.
Sampling: Frequency: at t= 0 h from freshly prepared solutions and at t= 24 h
from the freshly prepared solutions and the 24h-old solutions.
Volume: 10 ml from the approximate centre of the lest vessel.
Storage: Not applicable, samples were analysed on the day of sampling.
Additionally, reserve samples of 10 ml were taken from all concentrations. These samples were
stored in a deep-freeze for possible analysis until delivery of the final report with a maximum of
three months. Specification of the samples analysed and the method of analysis are described
in the appended Analytical Report.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Basedon the results of the range-finding test, a range of
nominal concentrations from 10 to 100 mg/1 increasing by
a factor of 1 .8.
Preparation during the static range-finding test started with a stock solution at 100 mg/l.
Treatment with ultrasonic waves was applied to dissolve the test substance in the test medium.
Thereafter, the stock solution was cooled to approximately 20 °C. The lower test concentraticns
were prepared by subsequent dilutions of this stock solution in test medium. Test solutions for
the semi-static final study were prepared similarly. However, test solutions were renewed after
24 hours of exposure. The final test solutions were all clear and colourless.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain: (Crustacea, Cladocera) (Straus, 1820)
- Age at study initiation (mean and range, SD): < 24 hours
- Method of breeding:
Start of each batch: With new-born animals. i.e. less than 3 days old, by
placing about 250 of them into 10 litres of medium in an all-glass culture vessel.
Maximum age of the cultures: 4 weeks
Renewal of the cultures: After 7 days of cultivation half of the medium twice a week.
Temperature of medium: 18-22°C, constant within ± 1°C
Feeding: Daily, a suspension of fresh water algae.
Medium: M7, as prescribed by Dr. Elendt-Schneider (Eiendt, B.P.,
1990: Selenium deficiency in Crustacea. An
ultrastructural approach to antennal darnage in
Daphnia magna Straus. Protoplasma 154, 25-33).
- Feeding during test: no
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Test temperature:
- 20.0 to 20.3°C
- pH:
- 7.4 - 8.0
- Dissolved oxygen:
- 7.5 - 9.0
- Nominal and measured concentrations:
- norminal: 10, 18, 32, 56 and 100 mg/l
measured 5.7; 11.7, 22.4, 41.8 and 76.6 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml, all-glass
- fill volume: 80 ml
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): after 24 h
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO, prepared in milli-RO water
OTHER TEST CONDITIONS
- Photoperiod: 16 hours photoperiod daily - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 14 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Results with reference substance (positive control):
- The actual responses in this reference test with K2Cr207 are within the ranges of the expected
responses at the different concentrations. Hence, the sensitivity of this batch of D. magna was
in agreement with the historical data collected at NOTOX.
The 24h-EC50 was > 1.8 mg/l.
The 48h-EC50 was 0.95 mg/l with 95% fiducial limits of 0.78- 1.3 mg/l.
Any other information on results incl. tables
ACCEPTABILITY OF THE TEST
1. ln the controls, no daphnia became immobilised or trapped at the surface of the water.
2. The analytical program showed that the actual test concentrations could not be maintained at 80% of the initial concentration, despite renewal of the test solutions after 24 hours of exposure. Hence, determination of the EC50-values was based on the average exposure concentrations, i.e. the mean of the concentrations measured at the start and after 24 hours of exposure.
3. Further, all test conditions remained within the ranges prescribed by the protocol.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 values were based on the average exposure concentrations expressed as mg/l.
Under the conditions of the present study, the EC50 values for mobility of Daphnia magna
exposed to 3,5-DIACETOXYACETOPHENON were as follows:
The 24h-EC50 was 89 mg/1 with a 95% confidence interval between 73 and 130 mg/1.
The 48h-EC50 was 14 mg/1 with a 95% confidence interval between 11 and 18 mg/1.
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