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EC number: 406-200-8 | CAS number: 117827-06-2 COUPLER II; UC-136
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 1994, december 13th to 1995, january 11th
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study performed according to the Test Guidelines described in the EEC Directive 84/449/EEC.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3',5'-dichloro-4'-ethyl-2'-hydroxypalmitanilide
- EC Number:
- 406-200-8
- EC Name:
- 3',5'-dichloro-4'-ethyl-2'-hydroxypalmitanilide
- Cas Number:
- 117827-06-2
- Molecular formula:
- C24H39Cl2NO2
- IUPAC Name:
- N-(3,5-dichloro-4-ethyl-2-hydroxyphenyl)hexadecanamide
- Details on test material:
- A bulk sample (stated weight 300 g) of the test material was
supplied by the Study Sponsor, arriving at Pharmaco LSR 26 July
1994. The test material was cream in colour and granular in form
and was identified as M-3221, batch number 944002. The sample was
stored at ambient temperature and protected from light until use
in testing.
The identity, strength and purity of the test material received
and its stability under the storage conditions were the
responsibility of the Sponsor.
Nominal exposure concentrations quoted in this report refer to the
test material as received; no allowance has been made for a purity
of less than 100%.
Constituent 1
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Axenic, uni-algal slope cultures of Selenastrum
capricornutum, CCAP number 278/4 were obtained from the
Culture Collection of Algae and Protozoa (CCAP), Freshwater
Biological Association, Ferry House, Ambleside, Cumbria,
2 November 1994. These slopes were stored in an
illuminated incubator nominally at 5°C until required.
Initially, liquid cultures for the test inoculum were
established by inoculation of OECD mineral salts medium
(50 ml; Appendix 1) with cells aseptically removed from
slope cultures. Subsequently, subcultures were prepared
from these liquid cultures by the aseptic transfer of
aliquots into fresh OECD medium, which was then incubated
for several days, until the required cell density had been
achieved.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- Ammonium chloride (NH4Cl) 15.0 mg/l
Magnesium chloride (MgCl2.6H20) 12.0 mg/l
Calcium chloride (CaCl2.2H20) 18.0 mg/l
Magnesium sulphate (MgSO4.7H20) 15.0 mg/l
Potassium dihydrogen orthophosphate (KH2PO4) 1.6 mg/l
Ferric chloride (FeCl3.6H20) 0.08 mg/l
EDTA disodium salt (Na2EDTA.2H20) 0.1 mg/l
Boric acid (H3B03) 0.185 mg/l
Manganous chloride (MnCl2.4H20) 0.415 mg/l
Zinc chloride (ZnCl2) 0.003 mg/l
Cobaltous chloride (C0Cl2.6H20) 0.0015 mg/l
Cupric chloride (CuCl2.2H20) 0.00001 mg/l
Sodium molybdate (Na2MoO4.2H20) 0.007 mg/l
Sodium hydrogen carbonate (NaHCO3) 50.0 mg/l - Test temperature:
- The temperature of the incubator ranged from 21.2 to 24.8°C during
the test.
The temperatures of the contents of control and test flasks at the
start of the test ranged between 21.3 and 21.8°C; after 72 hours,
temperatures ranged from 22.4 to 22.8°C. - pH:
- The pH of the contents of the control and test flasks measured at
the start of the test ranged from 7.4 to 7.6 respectively; after
72 hours pH ranged between 7.3 and 7.8. - Nominal and measured concentrations:
- nominal concentration : 0.1, 0.2, 0.5, 1, 2, 5 and 10 mg/l
measured concentrations : 0 and 5,68 mg/l - Details on test conditions:
- On the day of preparation, the test medium was clear and
colourless with particulate material visible on its surface and on
the bases of the vessels. - Reference substance (positive control):
- yes
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 5.68 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 5.68 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Details on results:
- Cell counts in control and test cultures in the rangefinding test
indicated that the 72-hour EC5O of M-3221 to Selenastrum capricornutum
was greater than 100 mg/l (nominal value).
The results of chemical analysis indicated that M-3221 was poorly
soluble in the mineral salts medium; analysis of samples of filtered
media was not attempted because a method for extracting M-3221 from
water at low levels (< 1 mg/l) could not be identified. Based on
visual observations, the limit of aqueous solubility was less than
1 mg/l. Analysis of unfiltered samples of test media indicated that at
1 and 10 mg/l the levels achieved were adequately maintained; measured
concentrations at the other levels were variable.
4.1 Chemical analysis
The results indicate that the exposure concentration, although
lower than intended (52% of its nominal value), was adequately
maintained during the test; the overall mean measured level of
M-3221 was 5.68 mg/l.
4.2 Algal growth
Exposure of Selenast rum capricornutum to M-3221 at a mean measured
concentration of 5.68 mg/l did not result in a significant
reduction (p >0.05) in either the average specific growth rates or
biomass of test cultures compared to solvent control cultures.
Thus, the no-observed-effect concentration (NOEC) for growth rate
and biomass was not identified but must be >5.68 mg/l and the
median effect concentrations (ErC5O and EbC5O) could not be
calculated.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- valid study
- Conclusions:
- Cell counts in control and test cultures in the rangefinding test
indicated that the 72-hour EC5O of M-3221 to Selenastrum capricornutum
was greater than 100 mg/l (nominal value).
The results of chemical analysis indicated that M-3221 was poorly
soluble in the mineral salts medium; analysis of samples of filtered
media was not attempted because a method for extracting M-3221 from
water at low levels (< 1 mg/i) could not be identified. Based on
visual observations, the limit of aqueous solubility was less than
1 mg/i. Analysis of unfiltered samples of test media indicated that at
1 and 10 mg/i the levels achieved were adequately maintained; measured
concentrations at the other levels were variable.
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